Linear poly-thymine probe-based coupling of autocatalytic target recycling with nonlinear DNA assembly for label-free detection of microRNAs

2022 ◽  
pp. 107162
Author(s):  
Feng Sun ◽  
Jing Zhang ◽  
Lei Ge ◽  
Sihan Liu ◽  
Ting Zhu ◽  
...  
Keyword(s):  
Dna Assembly ◽  
Label Free ◽  
Target Recycling ◽  
Label Free Detection ◽  
Linear Poly

Coupling strand extension/excision amplification with target recycling enables highly sensitive and aptamer-based label-free sensing of ATP in human serum

The Analyst ◽  
2020 ◽  
Vol 145 (2) ◽  
pp. 434-439 ◽  
Author(s):  
Lin Xu ◽  
Bingying Jiang ◽  
Wenjiao Zhou ◽  
Ruo Yuan ◽  
Yun Xiang
Keyword(s):  
Human Serum ◽  
Signal Enhancement ◽  
Label Free ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Recycling Amplification

The integration of strand extension and excision recycling amplification leads to substantial signal enhancement for highly sensitive and label-free detection of ATP.

scholarly journals Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4390 ◽  
Author(s):  
Moustafa T. Gabr ◽  
F. Christopher Pigge
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Low Cost ◽  
Dnase I ◽  
Dna Assembly ◽  
Label Free ◽  
Serum Samples ◽  
Near Ir ◽  
Dinuclear Platinum ◽  
Label Free Detection

Switchable luminescent bioprobes whose emission can be turned on as a function of specific enzymatic activity are emerging as important tools in chemical biology. We report a promising platform for the development of label-free and continuous enzymatic assays in high-throughput mode based on the reversible solvent-induced self-assembly of a neutral dinuclear Pt(II) complex. To demonstrate the utility of this strategy, the switchable luminescence of a dinuclear Pt(II) complex was utilized in developing an experimentally simple, fast (10 min), low cost, and label-free turn-on luminescence assay for the endonuclease enzyme DNAse I. The complex displays a near-IR (NIR) aggregation-induced emission at 785 nm in aqueous solution that is completely quenched upon binding to G-quadruplex DNA from the human c-myc oncogene. Luminescence is restored upon DNA degradation elicited by exposure to DNAse I. Correlation between near-IR luminescence intensity and DNAse I concentration in human serum samples allows for fast and label-free detection of DNAse I down to 0.002 U/mL. The Pt(II) complex/DNA assembly is also effective for identification of DNAse I inhibitors, and assays can be performed in multiwell plates compatible with high-throughput screening. The combination of sensitivity, speed, convenience, and cost render this method superior to all other reported luminescence-based DNAse I assays. The versatile response of the Pt(II) complex to DNA structures promises broad potential applications in developing real-time and label-free assays for other nucleases as well as enzymes that regulate DNA topology.

scholarly journals DNA nanotechnology assisted nanopore-based analysis

2020 ◽  
Vol 48 (6) ◽  
pp. 2791-2806 ◽  
Author(s):  
Taoli Ding ◽  
Jing Yang ◽  
Victor Pan ◽  
Nan Zhao ◽  
Zuhong Lu ◽  
...  
Keyword(s):  
Single Molecule ◽  
Dna Nanotechnology ◽  
Clinical Diagnostics ◽  
Dna Assembly ◽  
Label Free ◽  
One Pot ◽  
Dna Strand Displacement ◽  
Label Free Detection ◽  
Target Molecules ◽  
Dna Strand

Abstract Nanopore technology is a promising label-free detection method. However, challenges exist for its further application in sequencing, clinical diagnostics and ultra-sensitive single molecule detection. The development of DNA nanotechnology nonetheless provides possible solutions to current obstacles hindering nanopore sensing technologies. In this review, we summarize recent relevant research contributing to efforts for developing nanopore methods associated with DNA nanotechnology. For example, DNA carriers can capture specific targets at pre-designed sites and escort them from nanopores at suitable speeds, thereby greatly enhancing capability and resolution for the detection of specific target molecules. In addition, DNA origami structures can be constructed to fulfill various design specifications and one-pot assembly reactions, thus serving as functional nanopores. Moreover, based on DNA strand displacement, nanopores can also be utilized to characterize the outputs of DNA computing and to develop programmable smart diagnostic nanodevices. In summary, DNA assembly-based nanopore research can pave the way for the realization of impactful biological detection and diagnostic platforms via single-biomolecule analysis.

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

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