Protein encapsulation in functionalized sol-gel silica: Effect of the encapsulation method on the release kinetics and the activity

We report the preparation of mesoporous silica nanoparticles covered by layer by layer (LbL) oppositely charged weak polyelectrolytes, comprising poly(allylamine hydrochloride) (PAH) and a sodium alginate, highly grafted by N-isopropylacrylamide/N-tert-butylacrylamide random copolymers, NaALG-g-P(NIPAM90-co-NtBAM10) (NaALG-g). Thanks to the pH dependence of the degree of ionization of the polyelectrolytes and the LCST-type thermosensitivity of the grafting chains of the NaALG-g, the as-prepared hybrid nanoparticles (hNP) exhibit pH/thermo-responsive drug delivery capabilities. The release kinetics of rhodamine B (RB, model drug) can be controlled by the number of PAH/NaALG-g bilayers and more importantly by the environmental conditions, namely, pH and temperature. As observed, the increase of pH and/or temperature accelerates the RB release under sink conditions. The same NaALG-g was used as gelator to fabricate a hNP@NaALG-g hydrogel composite. This formulation forms a viscous solution at room temperature, and it is transformed to a self-assembling hydrogel (sol-gel transition) upon heating at physiological temperature provided that its Tgel was regulated at 30.7 °C, by the NtBAM hydrophobic monomer incorporation in the side chains. It exhibits excellent injectability thanks to its combined thermo- and shear-responsiveness. The hNP@NaALG-g hydrogel composite, encapsulating hNP covered with one bilayer, exhibited pH-responsive sustainable drug delivery. The presented highly tunable drug delivery system (DDS) (hNP and/or composite hydrogel) might be useful for biomedical potential applications.

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Release kinetics of anti-inflammatory drug, and characterization and bioactivity of SiO2+PCL hybrid material synthesized by  sol-gel processing

Journal of Applied Biomaterials & Functional Materials ◽

10.5301/jabfm.5000160 ◽

2014 ◽

Vol 12 (3) ◽

pp. 218-227 ◽

Cited By ~ 3

Author(s):

Michelina Catauro ◽

Flavia Bollino

Keyword(s):

Hybrid Material ◽

Release Kinetics ◽

Sol Gel ◽

Inflammatory Drug ◽

Anti Inflammatory ◽

Gel Processing ◽

Kinetics Of

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Sol-gel processing of drug delivery materials and release kinetics

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-005-6688-x ◽

2005 ◽

Vol 16 (3) ◽

pp. 261-265 ◽

Cited By ~ 36

Author(s):

F. De Gaetano ◽

L. Ambrosio ◽

M. G. Raucci ◽

A. Marotta ◽

M. Catauro

Keyword(s):

Drug Delivery ◽

Release Kinetics ◽

Sol Gel ◽

Gel Processing

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Effect of Thermal Treatment of Sol-Gel Titania Reservoirs on Release Kinetics of Phenytoin

Journal of the American Ceramic Society ◽

10.1111/j.1551-2916.2012.05190.x ◽

2012 ◽

Vol 95 (6) ◽

pp. 2022-2027

Author(s):

Anna Kozina ◽

Karla A. Espinoza ◽

Emma Ortiz-Islas ◽

Ignacio A. Rivero

Keyword(s):

Thermal Treatment ◽

Release Kinetics ◽

Sol Gel ◽

Kinetics Of

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Antibacterial Activity and Iron Release of Organic-Inorganic Hybrid Biomaterials Synthesized via the Sol-Gel Route

Applied Sciences ◽

10.3390/app11199311 ◽

2021 ◽

Vol 11 (19) ◽

pp. 9311

Author(s):

Michelina Catauro ◽

Ylenia D’Errico ◽

Antonio D’Angelo ◽

Ronald J. Clarke ◽

Ignazio Blanco

Keyword(s):

Hybrid Materials ◽

Release Kinetics ◽

Sol Gel ◽

Antibacterial Properties ◽

Iron Release ◽

Biological Characterization ◽

Visible Spectroscopy ◽

E Coli ◽

Hybrid Biomaterials ◽

Kinetics Of

The aim of this work was the synthesis of hybrid materials of iron (II)-based therapeutic systems via the sol-gel method. Increasing amounts of polyethylene glycol (PEG 6, 12, 24, 50 wt%) were added to SiO2/Fe20 wt% to modulate the release kinetics of the drug from the systems. Fourier-transform infrared (FTIR) spectroscopy was used to study the interactions between different components in the hybrid materials. The release kinetics in a simulated body fluid (SBF) were investigated, and the amount of Fe2+ released was detected via ultraviolet-visible spectroscopy (UV-Vis) after reaction with ortho-phenanthroline. Furthermore, biological characterization was carried out. The bioactivity of the synthesized hybrid materials was evaluated via the formation of a layer of hydroxyapatite on the surface of samples soaked in SBF using spectroscopy. Finally, the potential antibacterial properties of seven different materials against two different bacteria—E. coli and S. aureus—were investigated.

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Kinetika Release Mikrokapsul Oleoresin Jahe Merah

REAKTOR ◽

10.14710/reaktor.16.3.128--140 ◽

2017 ◽

Vol 16 (3) ◽

pp. 128

Author(s):

Jayanudin Jayanudin ◽

R Rochmadi ◽

Meri Yulvianti ◽

Ahmad Imanudin ◽

Tri Rina Sari

Keyword(s):

Release Kinetics ◽

Crosslinking Agent ◽

Spray Dryer ◽

Model Order ◽

Negative Effect ◽

Pharmaceutical Industries ◽

Encapsulation Method ◽

Kinetic Release ◽

Red Ginger ◽

Kinetics Of

Abstract RELEASE KINETICS OF RED GINGER OLEORESIN MICROCAPSULES. Red ginger oleoresin microcapsule is used to protect the active component of medicine against the negative effect of the environment, thus the microcapsule can be applied in the pharmaceutical industries. Kinetic release is used to determine the rate of red ginger oleoresin microcapsule release in human body system. The models used for this purpose are zero order, first order, Higuchi, and Korsmeyer-Peppas. These models were completed by using graphical method to get the determination coefficient (R2). The aims of this research are to determine the effect of Tripolyphosphate (TPP) concentration towards microcapsule release, to determine the red ginger oleoresin microcapsule kinetic release, and to characterize the morphology and particle sizes of red ginger oleoresin microcapsule using the Scanning Electron Microscopy (SEM). The encapsulation method was started by emulsifying process of chitosan-alginate, with various ratios of Chitosan-Alginate such as 1:2, 1:1, 2:1, and 20 gram red ginger oleoresin, and also the addition of TPP, with various concentrations for as much as 3%, 4 %, and 5%, as the crosslinking agent. The emulsion was forwarded to the spray dryer machine to produce the red ginger oleoresin microcapsule. Then, the microcapsule was treated with the mixture of KH2PO4 and NaOH (pH 6.8) for 1 hour, and sample was taken for every 10 minutes for the dissolution test. The result showed that the increase of TPP concentration will reduce the release velocity of the active compound. Kinetic model with the highest value of R2 was the Korsmeyer-Peppas model. Keywords: alginate; encapsulation; release kinetics; chitosan red ginger oleoresin; Abstrak Mikrokapsul digunakan untuk melindungi oleoresin jahe merah yang sensitif terhadap pengaruh lingkungan, sehingga dapat diaplikasikan dalam bidang farmasi. Kinetika release digunakan untuk menentukan kecepatan release mikrokapsul oleoresin jahe merah, model yang digunakan adalah model order nol, order satu, model Higuchi, dan Model Korsmeyer-Peppas. Penyelesaian model-model ini menggunakan metode grafis dengan menentukan koefisien determinasi (R2). Tujuan penelitian ini adalah menentukan pengaruh konsentrasi sodium tripolifosfat (TPP) dan rasio berat antara kitosan dan alginate sebagai penyalut terhadap kinetika release dan karakterisasi morfologi mikrokapsul oleoresin jahe merah. Metode enkapsulasi dimulai dengan pembentukan emulsi dari penyalut kitosan-alginat dengan rasio 1:2; 1:1; 2:1 dan 20 gram oleoresin jahe merah, penambahan TPP 3%, 4% dan 5% untuk cross-link. Emulsi dialirkan ke spray dryer untuk menghasilkan mikrokapsul oleoresin jahe merah. Uji disolusi menggunakan medium basa dengan mencampurkan KH2PO4 dan NaOH (pH 6,8) sampel diambil tiap 10 menit selama 1 jam. Hasil penelitian yang telah dilakukan didapat peningkatan konsentrasi TPP akan menurunkan kecepatan releasenya, model model kinetika dengan nilai R2 terbesar adalah model Korsmeyer-Peppas. Kata kunci: alginat; enkapsulasi; kinetika release; kitosan; oleoresin jahe merah

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