Protein Encapsulation
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2021 ◽  
Vol 448 ◽  
pp. 214188
Author(s):  
Ana V. Almeida ◽  
Ana J. Carvalho ◽  
Alice S. Pereira

Langmuir ◽  
2021 ◽  
Author(s):  
Paola Astolfi ◽  
Elisabetta Giorgini ◽  
Diego Romano Perinelli ◽  
Francesco Vita ◽  
Fabrizio Corrado Adamo ◽  
...  

2021 ◽  
Vol 22 (13) ◽  
pp. 7134
Author(s):  
Michael Mertz ◽  
Kathrin Castiglione

Hollow vesicles made from a single or double layer of block-copolymer molecules, called polymersomes, represent an important technological platform for new developments in nano-medicine and nano-biotechnology. A central aspect in creating functional polymersomes is their combination with proteins, especially through encapsulation in the inner cavity of the vesicles. When producing polymersomes by techniques such as film rehydration, significant proportions of the proteins used are trapped in the vesicle lumen, resulting in high encapsulation efficiencies. However, because of the difficulty of scaling up, such methods are limited to laboratory experiments and are not suitable for industrial scale production. Recently, we developed a scalable polymersome production process in stirred-tank reactors, but the statistical encapsulation of proteins resulted in fairly low encapsulation efficiencies of around 0.5%. To increase encapsulation in this process, proteins were genetically fused with hydrophobic membrane anchoring peptides. This resulted in encapsulation efficiencies of up to 25.68%. Since proteins are deposited on the outside and inside of the polymer membrane in this process, two methods for the targeted removal of protein domains by proteolysis with tobacco etch virus protease and intein splicing were evaluated. This study demonstrates the proof-of-principle for production of protein-functionalized polymersomes in a scalable process.


Author(s):  
Rémi G. Tilkin ◽  
Julien G. Mahy ◽  
Nicolas Régibeau ◽  
Romain Vandeberg ◽  
Ana P. F. Monteiro ◽  
...  

2021 ◽  
Author(s):  
Yue Zhang ◽  
Daowen Zhang ◽  
Jin-Tao Wang ◽  
Xiaojie Zhang ◽  
Yongfang Yang

Stimuli-responsive nanogels were fabricated by reaction of proteins and polymers without using small-organic-molecules. Once the nanogels dissociated, the proteins were released with functional groups, secondary structures, and activities maintained.


2021 ◽  
Author(s):  
◽  
José Alvarez-Barreto

Dialdehyde starches (DAS) have been used as biomaterials due to their biocompatibility and biodegradability; nonetheless, sweet potato (Ipomea batatas L.) starch has not been researched. Films based on sweet potato DAS, mixed with native starch (NS), poly-vinyl alcohol (PVA) and glycerin have been developed with protein encapsulation, using central composite design (CCD) and response surface methodology (RSM). Input variables were oxidation degree, NS concentration and polymeric mixture volume, while output variables were film's thickness, equilibrium swelling and BSA (Bovine serum albumin) release. DAS was obtained through hydrogen peroxide (H2O2) oxidation, and the oxidation degree is referred to as H2O2 concentration. Films presented rough surfaces, and formulations containing 10% H2O2 DAS presented micropores. Water uptake was greater with higher DAS content. Film thickness depended on the volume of the polymeric suspension and influenced swelling capacity. According to RSM, the optimal formulation was DAS with 5% H2O2 and 35% NS. These results demonstrate that oxidized sweet potato starch has potential for protein encapsulation and delivery.


Soft Matter ◽  
2021 ◽  
Author(s):  
Nicholas A. Zervoudis ◽  
Allie C. Obermeyer

Charge patterned polypeptides modulate the complex coacervation of globular proteins with polymers. These protein coacervates have applications in protein encapsulation and delivery and in determining the function of biomolecular condensates.


2020 ◽  
Vol 308 ◽  
pp. 110502
Author(s):  
Rémi G. Tilkin ◽  
Xavier Colle ◽  
Anthony Argento Finol ◽  
Nicolas Régibeau ◽  
Julien G. Mahy ◽  
...  

2020 ◽  
Vol 3 (11) ◽  
pp. 2070025
Author(s):  
Changjun Yu ◽  
Faqing Huang ◽  
Warren A. Chow ◽  
Galen Cook‐Wiens ◽  
Xiaojiang Cui

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