Probing the surface ultrastructure of Brevibacillus laterosporus using atomic force microscopy

Micron ◽  
2020 ◽  
Vol 131 ◽  
pp. 102827 ◽  
Author(s):  
Khalid Alzahrani ◽  
Arun Kumar Shukla ◽  
Javed Alam ◽  
Abdurahman A. Niazy ◽  
Abdullah M. Alsouwaileh ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Ultrastructure ◽  
Force Microscopy ◽  
Brevibacillus Laterosporus ◽  
Atomic Force

scholarly journals Atomic force microscopy of a ctpA mutant in Rhizobium leguminosarum reveals surface defects linking CtpA function to biofilm formation

Microbiology ◽  
2011 ◽  
Vol 157 (11) ◽  
pp. 3049-3058 ◽  
Author(s):  
Jun Dong ◽  
Karla S. L. Signo ◽  
Elizabeth M. Vanderlinde ◽  
Christopher K. Yost ◽  
Tanya E. S. Dahms
Keyword(s):  
Atomic Force Microscopy ◽  
Mutant Strain ◽  
Biofilm Formation ◽  
Rhizobium Leguminosarum ◽  
Surface Defects ◽  
Ion Concentration ◽  
Adhesive Properties ◽  
Surface Ultrastructure ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy was used to investigate the surface ultrastructure, adhesive properties and biofilm formation of Rhizobium leguminosarum and a ctpA mutant strain. The surface ultrastructure of wild-type R. leguminosarum consists of tightly packed surface subunits, whereas the ctpA mutant has much larger subunits with loose lateral packing. The ctpA mutant strain is not capable of developing fully mature biofilms, consistent with its altered surface ultrastructure, greater roughness and stronger adhesion to hydrophilic surfaces. For both strains, surface roughness and adhesive forces increased as a function of calcium ion concentration, and for each, biofilms were thicker at higher calcium concentrations.

Cell wall architecture of Physcomitrella patens is revealed by atomic force microscopy

Botany ◽  
2008 ◽  
Vol 86 (4) ◽  
pp. 385-397 ◽  
Author(s):  
Haley D.M. Wyatt ◽  
Neil W. Ashton ◽  
Tanya E.S. Dahms
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Physcomitrella Patens ◽  
Surface Ultrastructure ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cell Wall Ultrastructure ◽  
Detailed Surface ◽  
Wall Ultrastructure ◽  
Cell Wall Development

The moss Physcomitrella patens (Hedw.) Bruch & Schimp. in B.S.G. serves as a nonvascular plant model system suitable for studying many plant developmental phenomena. The tip-growing filamentous protonemal stage of its life cycle exhibits polarized growth and various tropic responses. Conventional staining and light microscopy (LM) were used to provide the first direct evidence that protonemal cells of P. patens lack a cuticle. Atomic force microscopy (ATM) images reveal detailed surface structures identified by scanning electron microscopy (SEM). The cell wall ultrastructure is characterized by rounded protrusions that are uniformly distributed along each caulonemal filament, and longer fibrillar structures, which are disorganized at the apex, but become oriented in longitudinal arrays parallel to the growth axis in more proximal regions of caulonemal apical cells. The subapical cells are characterized by a polylamellated texture. There was no difference in gross surface ultrastructure between light-grown and dark-grown filaments, but the dimensions of the rounded protrusions at the apices of caulonemata cultured in the light and in darkness were significantly different. The convex and concave cell wall surfaces of a curved, gravitropically responding dark-grown caulonema appear structurally different. This investigation is the first to use AFM to probe the cell wall ultrastructure of a bryophyte. The data further elaborate a simple model of cell wall development in the caulonemata of P. patens that was proposed for other tip-growing filamentous plants.

scholarly journals Surface ultrastructure and mechanical property of human chondrocyte revealed by atomic force microscopy

2008 ◽  
Vol 16 (4) ◽  
pp. 480-488 ◽  
Author(s):  
C.-H. Hsieh ◽  
Y.-H. Lin ◽  
S. Lin ◽  
J.-J. Tsai-Wu ◽  
C.H. Herbert Wu ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Atomic Force Microscopy ◽  
Surface Ultrastructure ◽  
Force Microscopy ◽  
Human Chondrocyte ◽  
Atomic Force

scholarly journals Surface ultrastructure of SARS coronavirus revealed by atomic force microscopy

2005 ◽  
Vol 7 (12) ◽  
pp. 1763-1770 ◽  
Author(s):  
Shiming Lin ◽  
Chih-Kung Lee ◽  
Shih-Yuan Lee ◽  
Chuan-Liang Kao ◽  
Chii-Wann Lin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Sars Coronavirus ◽  
Surface Ultrastructure ◽  
Force Microscopy ◽  
Atomic Force

Atomic Force Microscopy and Scanning Electron Microscopy Reveal Genome–dependent Ultrastructure of Seed Surface

2001 ◽  
Vol 7 (6) ◽  
pp. 526-529
Author(s):  
T. Guha ◽  
R. Bhar ◽  
V. Ganesan ◽  
A. Sen ◽  
R.L. Brahmachary
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Contact Mode ◽  
Surface Ultrastructure ◽  
Force Microscopy ◽  
Seed Surface ◽  
Atomic Force ◽  
Different Strains ◽  
Scanning Electron

AbstractBoth scanning electron microscopy (SEM) and contact mode imaging via atomic force microscopy (AFM) have been utilized to elucidate the ultrastructure of mung bean seed surfaces. The results indicate: 1) that AFM is useful in the examination of seed surface ultrastructure ex-vaccuo without the need for additional complex preparative procedures; and 2) that both the cotyledon and seed coat of different strains of mung beans bear specific ultrastructural details unique to each strain. To our knowledge, these are the first AFM images of seed surfaces.

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