scholarly journals Correlates of hematuria on glomerular histology and electron microscopy in IgA nephropathy

2016 ◽  
Vol 72 (2) ◽  
pp. 120-124
Author(s):  
Rohit Tewari ◽  
Ritambhra Nada ◽  
Maninder Kaur ◽  
Puja Dudeja ◽  
Charan Singh Rayat ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Iga Nephropathy

Immunoglobulin A-dominant post-infectious glomerulonephritis

2018 ◽  
Author(s):  
Bernardo Rodriguez-Iturbe ◽  
Mark Haas
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Nephrotic Syndrome ◽  
Risk Factor ◽  
Iga Nephropathy ◽  
Immunoglobulin A ◽  
Renal Lesion ◽  
Crescent Formation ◽  
Histological Features ◽  
Post Infectious

Immunoglobulin A (IgA)-dominant post-infectious glomerulonephritis is usually associated with infections with Staphylococcus aureus. It is most commonly seen in patients over 60, and particularly in men. The renal lesion is acute and severe, and commonly includes crescent formation, although the described histological features vary widely. IgA is the dominant immunoglobulin and in later phases when capillary deposits are resolving it may be impossible to distinguish the condition from IgA nephropathy without the use of electron microscopy. Diabetes appears to be a risk factor. Complement levels are frequently low but may be normal. Clinically there is often severe nephrotic syndrome and hypertension may be less prominent.

The Value of Electron Microscopy in the Diagnosis of IgA Nephropathy

2002 ◽  
Vol 26 (4) ◽  
pp. 203-210 ◽  
Author(s):  
Xin Gu ◽  
Guillermo A. Herrera
Keyword(s):  
Electron Microscopy ◽  
Iga Nephropathy

scholarly journals Temporal and Spatial Expression of Podocyte-Associated Molecules Are Accompanied by Proteinuria in IgA Nephropathy Rat Model

2013 ◽  
pp. 35-45 ◽  
Author(s):  
H.-Y. LU ◽  
L.-Z. CHEN ◽  
X.-Y. JIANG ◽  
Y. MO ◽  
Y.-H. LING ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Iga Nephropathy ◽  
Rat Model ◽  
Light And Electron Microscopy ◽  
Urinary Protein ◽  
Real Time Quantitative Pcr ◽  
Renal Morphology ◽  
Healthy Control ◽  
Foot Process

We used a rat model to assess the role of nephrin, podocin, and desmin in the pathogenesis of IgA nephropathy (IgAN). A rat IgAN model was established by administration of BSA, CCl4, and lipopolysaccharide (LPS) and compared with healthy control rats. Urinary protein, urine red blood cells, and biochemical parameters were measured for 12 weeks. Renal morphology and ultrastructure were examined by light and electron microscopy. Immunofluorescence was used to assess IgA deposition in the glomeruli and to measure expression of nephrin, podocin, and desmin. Real-time quantitative PCR was used to measure expression of nephrin, podocin, and desmin mRNAs. IgAN rats developed proteinuria at week-6 and this worsened over time. Pathological changes were evident under light microscopy at week-8 and under electron microscopy at week-4. Immunofluorescence analysis showed deposition of IgA in the kidneys of IgAN rats, but not control rats. IgAN rats had increased expression of glomerular podocin, nephrin, and desmin mRNAs and proteins at week-4. The expression of nephrin, podocin and desmin proteins and the expression of podocin and desmin mRNAs preceded the increase in urinary protein. Taken together, our study of a rat model of IgAN indicates that changes in the expression and distribution of nephrin, podocin, and desmin precede and may cause foot process fusion and proteinuria.

scholarly journals Iga nephropathy

2004 ◽  
Vol 132 (1-2) ◽  
pp. 41-43
Author(s):  
Gordana Basta-Jovanovic
Keyword(s):  
Electron Microscopy ◽  
Nephrotic Syndrome ◽  
Light Microscopy ◽  
Iga Nephropathy ◽  
Immune Complex ◽  
Clinical Manifestations ◽  
Glomerular Disease ◽  
Glomerular Mesangium ◽  
Immune Deposits

IgA nephropathy is glomerular disease first described in 1968 by Berger, named after him Morbus Berger. The disease is characterized by the presence of IgA dominant or codominant imunoglobuline deposits in glomerular mesangium which can be demostrated by immunofluorescence. Clinical manifestations of IgA nephropathy in the majority of cases is hematuria which can be macro or microskopic, isolated or combined with proteinuria which can be of nephrotic range. In some cases nephrotic syndrome can be the first clinical presentatio. In 10% renal insuficiency can be present at the onset of the disease. By light microscopy IgA can manifest any of the histologie phenotypes of immune complex mediated prolifferative glomerulonephritis. According to light microscopy findings a classification systeme have been used to categorize the histologie patterns of IgA nephropathy. Glomerular changes in IgA nephropathy are prolifferative and can be focal or diffuse accompanied by crescentic formation in many cases. Immune deposits seen by electron microscopy appear as electron dense deposites most numerous in mesangium.

scholarly journals Clinicopathological and prognostic study of IgA-dominant postinfectious glomerulonephritis

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Ziyuan Huang ◽  
Bo Chen ◽  
Ying Zhou ◽  
Yan Liang ◽  
Wenxian Qiu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Iga Nephropathy ◽  
Deposition Rate ◽  
Selection Criteria ◽  
Proliferative Glomerulonephritis ◽  
Complement C3 ◽  
Mesangial Area ◽  
Postinfectious Glomerulonephritis ◽  
Endocapillary Proliferative Glomerulonephritis ◽  
Area Deposition

Abstract Background The clinicopathological and prognostic features of IgA-dominant postinfectious glomerulonephritis and its difference from the primary IgA nephropathy remains to be investigated. Methods The clinical and pathological data of 6542 patients who underwent renal biopsy from 2009 to 2020 in our hospital were reviewed and 50 patients who met the selection criteria of IgA-dominant postinfectious glomerulonephritis were enrolled to conduct a retrospective and observational single-center study. The selection criteria were: meet the characteristics of IgA dominance or codominance in immunofluorescence, and conform to 3 of the following 5 criteria: 1.Clinical or laboratory evidence show that there is infection before or at the onset of glomerulonephritis; 2.The level of serum complement decreased; 3.Renal pathology is consistent with endocapillary proliferative glomerulonephritis; 4. Glomerular immunofluorescence staining showed complement C3 dominance or codominance; 5. Hump-like subepithelial immune complex deposition was observed under electron microscopy. According to age, sex, renal function (estimated glomerular filtration rate, eGFR) and follow-up time, the control group was constructed with 1:3 matched cases of primary IgA nephropathy. The clinicopathological and prognostic differences between the two groups were analyzed. Results The most common histological pattern of IgA-dominant postinfectious glomerulonephritis was acute endocapillary proliferative glomerulonephritis and exudative glomerulonephritis. Immunofluorescence showed mainly IgA deposition or IgA deposition only, mainly deposited in the mesangial area (deposition rate 100 %), with typical C3 high-intensity staining (intensity++~+++), mainly deposited in the mesangial area (deposition rate 92.0 %). The fluorescence intensity of kappa is usually not weaker than lambda. The probability of the appearance of typical hump-like electron deposition under electron microscopy is low. Compared to primary IgA nephropathy, patients with IgA-dominant postinfectious glomerulonephritis had higher proportion of crescents (p = 0. 005) and endocapillary hypercellularity (p < 0.001) in pathological manifestations. Using serum creatinine level doubled of the baseline or reached end-stage renal disease as the endpoint, the prognosis of IgA-dominant postinfectious glomerulonephritis patients was worse than that of primary IgA nephropathy patients (p = 0.013). Conclusions The clinicopathological features of patients with IgA-dominant postinfectious glomerulonephritis was different from that of primary IgA nephropathy, and the prognosis was worse.

Platinum Compounds as Stains for Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 230-231
Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg
Keyword(s):  
Electron Microscopy ◽  
Hela Cells ◽  
Uranyl Acetate ◽  
Sarcoma 180 ◽  
Platinum Compounds ◽  
Kb Cells ◽  
En Bloc ◽  
Human Lymphoma ◽  
Ultrathin Sections ◽  
Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

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