Novel matrix metalloproteinase from the cyst nematodes Heterodera glycines and Globodera rostochiensis

2004 ◽  
Vol 136 (1) ◽  
pp. 109-112 ◽  
Author(s):  
Elena S Kovaleva ◽  
Edward P Masler ◽  
Andrea M Skantar ◽  
David J Chitwood
Keyword(s):  
Matrix Metalloproteinase ◽  
Heterodera Glycines ◽  
Globodera Rostochiensis ◽  
Cyst Nematodes

scholarly journals Study of European and Czech populations of potato cyst nematodes (Globodera rostochiensis and G. pallida) by RAPD method

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 70-74 ◽  
Author(s):  
P. Sedlák ◽  
M. Melounová ◽  
S. Skupinová ◽  
P. Vejl ◽  
J. Domkářová
Keyword(s):  
Animal Species ◽  
Molecular Genetic ◽  
Globodera Rostochiensis ◽  
Globodera Pallida ◽  
Genetic Identification ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Genetic Dissimilarity ◽  
Solanaceous Plants ◽  
Molecular Genetic Identification

Potato cyst nematodes (PCN) are the big problem in worldwide planting of potatoes and another Solanaceous plants. Identification of individual pathotypes according to international scheme is very demanding but a very important part of the phytosanitary process to control these pests. Molecular genetic identification of different plant and animal species or individuals is a very interesting way at the present time and let’s hope that it will be important in future. This report presents results of the RAPD study of nine different real PCN populations. There were five Globodera rostochiensis populations and four G. pallida populations. Pathotypes Ro2, Ro2/3, Ro4, Ro5, Pa2 and Pa3 were from European populations; population Ro1 and X were of Czech provenance. Genetics variable of these populations was described by a set of six decameric primers (OPA 07, OPG 03, OPG 05, OPG 08, OPG 10 and OPG 13). Genetic dissimilarity was by Gel Manager for Windows evaluated. Detectable differences behind all populations were found and the dendrogram was compiled. The unknown population X was sorted into group of Globodera pallida species subgroup of Pa2 consequently.

scholarly journals Variability of D2/D3 segment sequences of several populations and pathotypes of potato cyst nematodes (Globodera rostochiensis, Globodera pallida)

2010 ◽  
Vol 46 (No. 4) ◽  
pp. 171-180 ◽  
Author(s):  
O. Douda ◽  
M. Zouhar ◽  
E. Nováková ◽  
J. Mazáková ◽  
P. Ryšánek
Keyword(s):  
Dna Analysis ◽  
Pcr Amplification ◽  
Globodera Rostochiensis ◽  
Globodera Pallida ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Routine Diagnostics ◽  
Pair Comparisons ◽  
Single Fragment ◽  
Direct Management

Potato cyst nematodes (Globodera rostochiensis, Globodera pallida) remain a key pest in the main potato growing regions of the Czech Republic. Due to difficult direct management and presence of diverse pathotypes attacking different potato cultivars the rapid and reliable diagnostics is of crucial importance. Currently, efforts are aimed at a description of different pathotypes based on DNA analysis. The main objective of this study was to evaluate the homogeneity of sequences of D2/D3 segments of the 28S rDNA gene obtained from 3 populations of G. rostochiensis and 5 populations of G. pallida and estimate their value for diagnostic purposes. PCR amplification yielded a single fragment of the length of 700 bp approximately in all populations. The alignment score of the vast majority of all pair comparisons of G. rostochiensis and G. pallida populations varied from 98 to 99. In total 14 point deletions and 3 substitutions were observed. The variability of D2/D3 segments of potato cyst nematodes is rather low and this DNA region can be used for diagnostics on a species level because more differences were found after comparing with G. tabacum and G. millefolii sequences obtained from Gene Bank; however the applicability of D2/D3 sequences to routine diagnostics of potato cyst nematodes could be complicated by its similarity to corresponding sequences of the nematode G. artemisiae.

scholarly journals Morphological characteristics and distribution of Globodera species in Slovenia

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 354-357
Author(s):  
G. Urek ◽  
S. Širca ◽  
V. Meglič
Keyword(s):  
Hot Spot ◽  
Morphological Characteristics ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Globodera Pallida ◽  
Cyst Nematodes ◽  
First Time ◽  
Austrian Border ◽  
Central Slovenia

Surveillance on cyst nematodes in Slovenia started already in 1963. We have found Globodera rostochiensis for the first time in 1971 and for the second time in 1975 (two cysts). In 1999 cysts of yellow potato cyst nematode were found again. A small hot spot was detected along the Slovene Austrian border. Cysts of Globodera rostochiensis were extracted from samples taken in 1.6 hectare field. In 2001 we surveyed 194 fields with the surface of 468.29 ha. We discovered another hot spot of Globodera rostochiensis in the Central Slovenia. Cysts of Globodera achilleae are seldom discovered in Slovenia. During the period between 1980 and 2000 we extracted altogether 206 cysts. In 2001 we found three fields where we extracted at first seven cysts. With more detailed survey we extracted another 30 cysts. In a few soil samples taken from the imported potatoes from Italy we have intercepted cysts of Globodera pallida. For that reason shipments were returned. In a similar way in 2002 shipments from Croatia were returned because of G. rostochiensis presence. Specimens of Globodera rostochiensis and G. achilleae species found in Slovenia were morphometricaly handeled.

Repetitive DNA and hybridization patterns demonstrate extensive variability between the sibling species Globodera rostochiensis and G. pallida

Parasitology ◽  
1989 ◽  
Vol 99 (1) ◽  
pp. 133-138 ◽  
Author(s):  
A. J. De Jong ◽  
J. Bakker ◽  
M. Roos ◽  
F. J. Gommers
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Repetitive Dna ◽  
Sibling Species ◽  
Restriction Enzyme ◽  
Globodera Rostochiensis ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
High Molecular Weight Dna ◽  
Species Hybridization

SUMMARYA method is described for the isolation of high molecular weight DNA from females of potato cyst nematodes. Restriction enzyme analyses of repetitive DNA revealed 30 bands specific for Globodera rostochiensis and 8 bands specific for G. pallida. None of the repetitive DNA bands resolved was common to both species. Hybridization patterns, obtained with a DrosophilaβJ-tubulin probe, revealed 4 common bands, 3 bands specific for G. rostochiensis and 12 bands specific for G. pallida.

scholarly journals Morphological and molecular identification of the potato cyst nematodes Globodera rostochiensis and G. pallida in Portuguese potato fields

Nematology ◽  
2017 ◽  
Vol 19 (8) ◽  
pp. 883-889 ◽  
Author(s):  
Maria J. Camacho ◽  
Filomena Nóbrega ◽  
Arlindo Lima ◽  
Manuel Mota ◽  
Maria L. Inácio
Keyword(s):  
Multiplex Pcr ◽  
Molecular Identification ◽  
Diagnostic Technique ◽  
Nematode Species ◽  
Globodera Rostochiensis ◽  
Control Measures ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Widespread Species ◽  
Species Specific

The potato cyst nematodes (PCN) Globodera rostochiensis and G. pallida pose one of the greatest threats to potato crops worldwide and are subject to strict quarantine regulations in many countries. The identification of these Globodera species based on morphology may be ambiguous due to the variability of the main morphological features and the overlapping of the standard parameters in these two species; thus, confirmation via molecular methods is recommended. Multiplex PCR with species-specific primers (ITS5/PITSp4 + PITSr3) allows both species to be distinguished. However, despite the development of molecular identification methods, the morphological approach remains useful as a complementary diagnostic technique. In this work, we report results of morphological and molecular analyses that were carried out in two Globodera species from Portuguese potato fields. The average morphometric values of 40 cysts and 40 second-stage juveniles were generally within the expected ranges for G. pallida and G. rostochiensis with some variations noted. Molecular analysis with multiplex PCR confirmed the morphometric identification. The present results confirmed the occurrence of two potato cyst nematode species, G. rostochiensis and G. pallida. Surprisingly, the analysis of soils from Portuguese potato fields detected a greater number of samples infested with G. pallida, which is contrary to expectation as G. rostochiensis has been considered the most widespread species in Portugal. The distinction between the two species is therefore essential in order to detect their presence in the country with a view to re-evaluating the control measures implemented so far and adopting more effective practices.

The effects of α-solanine and zinc sulphate on the behaviour of potato cyst nematodes Globodera rostochiensis and G. pallida

Nematology ◽  
2015 ◽  
Vol 17 (9) ◽  
pp. 1105-1111 ◽  
Author(s):  
Vincas Būda ◽  
Rasa Čepulytė-Rakauskienė
Keyword(s):  
Host Plants ◽  
Zinc Sulphate ◽  
Globodera Rostochiensis ◽  
Globodera Pallida ◽  
The Other ◽  
Potato Cyst Nematodes ◽  
Water Control ◽  
Cyst Nematodes ◽  
Second Stage ◽  
Testing Period

Two behavioural assays were carried out: one on the attraction of potato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, to solanaceae-specific secondary metabolite α-solanine, the other on the effect of ZnSO4 (a compound known to suppress chemoreceptors). The first assay demonstrated that α-solanine was attractive to second-stage juveniles (J2) of PCN; G. pallida was attracted to concentrations of 10−4 M and 10−5 M, whereas G. rostochiensis was attracted to 10−5 M. Globodera pallida reacted faster than G. rostochiensis to the same concentration of α-solanine. As α-solanine is produced by host plants of PCN, this compound is attributed to kairomones. The response to α-solanine of nematodes pre-exposed to a 3 mM ZnSO4 solution was significantly suppressed compared to that of water control. The effect was observed throughout the whole testing period (30 min). This is the first evidence that both α-solanine and ZnSO4 can affect the behaviour of hatched J2 of PCN.

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