Enzymes for energy. Structural computational analysis, substrate association and product dissociation from the thermophilic esterase of Alicyclobacillus acidocaldarius: Implications in biodiesel production

2013 ◽  
Vol 97 ◽  
pp. 156-168 ◽  
Author(s):  
Chris G. Whiteley ◽  
Duu-Jong Lee
Keyword(s):  
Computational Analysis ◽  
Biodiesel Production ◽  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius

scholarly journals Alicyclobacillus acidocaldarius Thermophilic Esterase EST2's Activity in Milk and Cheese Models

2006 ◽  
Vol 72 (5) ◽  
pp. 3191-3197 ◽  
Author(s):  
Luigi Mandrich ◽  
Giuseppe Manco ◽  
Mos� Rossi ◽  
Esther Floris ◽  
Tanja Jansen-van den Bosch ◽  
...  
Keyword(s):  
Short Chain Fatty Acid ◽  
Hydrolytic Activity ◽  
Spectrophotometric Assay ◽  
Fatty Acid Esters ◽  
Optimal Conditions ◽  
Ester Synthesis ◽  
Thermophilic Enzyme ◽  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius ◽  
Acid Esters

ABSTRACT The aim of this work was to investigate the behavior of thermophilic esterase EST2 from Alicyclobacillus acidocaldarius in milk and cheese models. The pure enzyme was used to compare the EST2 hydrolytic activity to the activity of endogenous esterase EstA from Lactococcus lactis. The results indicate that EST2 exhibits 30-fold-higher esterase activity than EstA. As EstA has thioesterase activity, EST2 was assayed for this activity under the optimal conditions determined for EstA (namely, 30�C and pH 7.5). Although it is a thermophilic enzyme, EST2 exhibited eightfold-higher thioesterase activity than EstA with S-methyl thiobutanoate. The abilities of EST2 and EstA to synthesize short-chain fatty acid esters were compared. Two methods were developed to do this. In the first method a spectrophotometric assay was used to monitor the synthesis of esters by the pure enzymes using p-nitrophenol as the alcohol substrate. The synthetic activities were also evaluated under conditions that mimicked those present in milk and/or cheese. The second method involved evaluation of the synthetic abilities of the enzymes when they were directly added to a model cheese matrix. Substantial ester synthesis by EST2 was observed under both conditions. Finally, esterase and thioesterase activities were evaluated in milk using the purified EST2 enzyme and in the model cheese matrix using a strain of L. lactis NZ9000 harboring the EST2 gene and thus overproducing EST2. Both the esterase and thioesterase activities measured in milk and in the cheese matrix were much greater than the activities of the controls.

Enlarging the substrate portfolio of the thermophilic esterase EST2 from Alicyclobacillus acidocaldarius

Extremophiles ◽  
2015 ◽  
Vol 19 (5) ◽  
pp. 1001-1011 ◽  
Author(s):  
Angela Pennacchio ◽  
Luigi Mandrich ◽  
Giuseppe Manco ◽  
Antonio Trincone
Keyword(s):  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius

Irreversible inhibition of the thermophilic esterase EST2 from Alicyclobacillus acidocaldarius

Extremophiles ◽  
2008 ◽  
Vol 12 (5) ◽  
pp. 719-728 ◽  
Author(s):  
Ferdinando Febbraio ◽  
Sandro Esposito D’Andrea ◽  
Luigi Mandrich ◽  
Luigia Merone ◽  
Mosè Rossi ◽  
...  
Keyword(s):  
Irreversible Inhibition ◽  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius

Functional and structural features of the oxyanion hole in a thermophilic esterase from Alicyclobacillus acidocaldarius

2007 ◽  
Vol 71 (4) ◽  
pp. 1721-1731 ◽  
Author(s):  
Luigi Mandrich ◽  
Valeria Menchise ◽  
Vincenzo Alterio ◽  
Giuseppina De Simone ◽  
Carlo Pedone ◽  
...  
Keyword(s):  
Structural Features ◽  
Oxyanion Hole ◽  
Thermophilic Esterase ◽  
Alicyclobacillus Acidocaldarius

scholarly journals An Integrated Structural and Computational Study of the Thermostability of Two Thioredoxin Mutants from Alicyclobacillus acidocaldarius

2003 ◽  
Vol 185 (14) ◽  
pp. 4285-4289 ◽  
Author(s):  
Simonetta Bartolucci ◽  
Giuseppina De Simone ◽  
Stefania Galdiero ◽  
Roberto Improta ◽  
Valeria Menchise ◽  
...  
Keyword(s):  
Amino Acids ◽  
Computational Analysis ◽  
Computational Study ◽  
Electrostatic Energy ◽  
Alicyclobacillus Acidocaldarius ◽  
Mutant Forms

ABSTRACT We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.

Biodiesel production from non-edible oils: A review

2020 ◽  
pp. 149-159
Author(s):  
Jatinder Kataria ◽  
Saroj Kumar Mohapatra ◽  
Amit Pal
Keyword(s):  
Environmental Impact ◽  
Energy Demand ◽  
Fossil Fuels ◽  
World Wide ◽  
Edible Oils ◽  
Control Measure ◽  
Biodiesel Production ◽  
Pollution Level ◽  
Animal Fats ◽  
The World

The limited fossil reserves, spiraling price and environmental impact due to usage of fossil fuels leads the world wide researchers’ interest in using alternative renewable and environment safe fuels that can meet the energy demand. Biodiesel is an emerging renewable alternative fuel to conventional diesel which can be produced from both edible and non-edible oils, animal fats, algae etc. The society is in dire need of using renewable fuels as an immediate control measure to mitigate the pollution level. In this work an attempt is made to review the requisite and access the capability of the biodiesel in improving the environmental degradation.

PENGARUH PELARUT KLOROFORM DALAM PEMURNIAN GLISEROL DENGAN PROSES ASIDIFIKASI ASAM KLORIDA

2016 ◽  
Vol 5 (3) ◽  
pp. 38-43
Author(s):  
Windi Monica Surbakti ◽  
Gerson Rico M.H ◽  
Mersi Suriani Sinaga
Keyword(s):  
Fatty Acid ◽  
Volume Ratio ◽  
Biodiesel Production ◽  
Extraction Time ◽  
Glycerol Concentration ◽  
Purification Process ◽  
Soap Content ◽  
Test Volume ◽  
Base Content ◽  
Water Salt

Glycerol as a byproduct of biodiesel production was approximately formed 10% of the biodiesel weight. Impurities which contained in the glycerol such as catalyst, soap, methanol, water, salt, and matter organic non glycerol (MONG) have a significant effect on the glycerol concentration. So, it is necessary to treat the impurities. The purpose of this study is to know the effect of chloroform to glycerol purification process with acidification method using hydrochloric acid as pretreatment process. This research was begun with acid addition to the glycerol to neutralize the base content and to split the soap content into free fatty acid and salt, that are more easily separated from glycerol. Then the process was continued with extraction by the solvent chloroform using the variable of test volume ratio (v/v) (1:1, 1:1.5, 1:2)  and the extraction time (20, 40, and 60 minutes). The results showed that the more volume of solvent used, gave less extraction time to produce high purity of glycerol. The highest purity produced in this study amounted to 90,9082% is obtained at the ratio of the volume solvent (v/v) 1:1 with extraction time 60 minutes.

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