Molecular recognition of synthesized halogenated chalcone by calf thymus DNA through multispectroscopic studies and analysis the anti-cancer, anti-bacterial activity of the compounds

A new tin(IV) corrole, 5,10,15-tris(4-methoxycarbonylphenyl) corrole tin(IV) (1-Sn) was synthesized and characterized. The DNA binding, photocleavage and anti-cancer activity were studied and compared with its free-base. The interaction of 1-Sn and its free-base 1 with calf thymus DNA had been investigated by spectroscopic methods, viscosity measurements and molecular docking analysis. The results revealed that 1-Sn and 1 could interact with calf thymus DNA via an outside groove binding mode. Furthermore, although 1 displayed no photonuclease activity, 1-Sn exhibited good photonuclease activity as indicated by agarose gel electrophoresis, and superoxide anion might be the active intermediate for the DNA scission. Finally, 1 was nontoxic but 1-Sn displayed cytotoxicity towards A549 tumor cell lines.

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Effects of light-activated diazido-Pt IV complexes on cancer cells in vitro

Philosophical Transactions of The Royal Society A Mathematical Physical and Engineering Sciences ◽

10.1098/rsta.2012.0118 ◽

2013 ◽

Vol 371 (1995) ◽

pp. 20120118 ◽

Cited By ~ 26

Author(s):

Patrick J. Bednarski ◽

Katharina Korpis ◽

Aron F. Westendorf ◽

Steffi Perfahl ◽

Renate Grünert

Keyword(s):

Cell Death ◽

Cancer Cells ◽

Calf Thymus Dna ◽

Cross Resistance ◽

Cancer Drug ◽

Cancer Cell Growth ◽

Calf Thymus ◽

Anti Cancer ◽

Cellular Dna

Various Pt IV diazides have been investigated over the years as light-activatable prodrugs that interfere with cell proliferation, accumulate in cancer cells and cause cell death. The potencies of the complexes vary depending on the substituted amines (pyridine=piperidine>ammine) as well as the coordination geometry ( trans diazide> cis ). Light-activated Pt IV diazides tend to be less specific than cisplatin at inhibiting cancer cell growth, but cells resistant to cisplatin show little cross-resistance to Pt IV diazides. Platinum is accumulated in the cancer cells to a similar level as cisplatin, but only when activated by light, indicating that reactive Pt species form photolytically. Studies show that Pt also becomes attached to cellular DNA upon the light activation of various Pt IV diazides. Structures of some of the photolysis products were elucidated by LC–MS/MS; monoaqua- and diaqua-Pt II complexes form that are reactive towards biomolecules such as calf thymus DNA. Platination of calf thymus DNA can be blocked by the addition of nucleophiles such as glutathione and chloride, further evidence that aqua-Pt II species form upon irradiation. Evidence is presented that reactive oxygen species may be generated in the first hours following photoactivation. Cell death does not take the usual apoptotic pathways seen with cisplatin, but appears to involve autophagy. Thus, photoactivated diazido-Pt IV complexes represent an interesting class of potential anti-cancer agents that can be selectively activated by light and kill cells by a mechanism different to the anti-cancer drug cisplatin.

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Interaction Studies of a Novel, Water-Soluble and Anti-Cancer Palladim(II) Complex with Calf Thymus DNA

Bulletin of the Korean Chemical Society ◽

10.5012/bkcs.2010.31.02.435 ◽

2010 ◽

Vol 31 (2) ◽

pp. 435-441 ◽

Cited By ~ 16

Author(s):

H. Mansouri-Torshizi ◽

M. Saeidifar ◽

A. Divsalar ◽

A.A. Saboury ◽

S. Shahraki

Keyword(s):

Water Soluble ◽

Calf Thymus Dna ◽

Interaction Studies ◽

Calf Thymus ◽

Anti Cancer

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Molecular Transformations in DNA under the Influence of UV-radiation

Current Applied Polymer Science ◽

10.2174/2452271604666200814123505 ◽

2020 ◽

Vol 04 ◽

Author(s):

Vigen G. Barkhudaryan ◽

Gayane V. Ananyan ◽

Nelli H. Karapetyan

Keyword(s):

Uv Radiation ◽

Uv Irradiation ◽

Absorption Spectroscopy ◽

Calf Thymus Dna ◽

Dilute Solutions ◽

Concentrated Solutions ◽

Buffer Solution ◽

Low Concentration ◽

Calf Thymus ◽

Dna Solutions

Background: The processes of destruction and crosslinking of macromolecules occur simultaneously under the influence of ultraviolet (UV) radiation in synthetic polymers, dry DNA and their concentrated solutions. Objective: The effect of UV radiation on calf thymus DNA in dilute solutions subjected to UV- irradiation was studied in this work. Method: The calf thymus DNA was studied in dilute solutions using viscometry, absorption spectroscopy and electrophoresis. Results: It was shown, that at a low concentration of DNA in the buffer solution ([DNA] = 85 μg / ml) under the influence of UV radiation, the processes of destruction of macromolecules and an increase in their flexibility predominate, which is accompanied by a gradual decrease in the viscosity of their solution. In addition, due to the low concentration of the solution, intramolecular crosslinking of macromolecules predominates, which also reduces their size and, consequently, the viscosity of the solution. Conclusion: It was concluded, that in dilute DNA solutions, due to the predominance of the processes of intramolecular crosslinking of macromolecules over intermolecular, only constant processes of decreasing the sizes of DNA macromolecules occur. As a result, its solubility remains virtually unchanged during UV irradiation. The described comments are also excellently confirmed by the results of absorption spectroscopy and electrophoresis

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Ce(IV)-mediated formation of benzenediazonium ion from a non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) and its binding to DNA

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19892021 ◽

1989 ◽

Vol 54 (7) ◽

pp. 2021-2026

Author(s):

Marie Stiborová ◽

Befekadu Asfaw ◽

Pavel Anzenbacher

Keyword(s):

Azo Dyes ◽

Acidic Medium ◽

Model System ◽

Calf Thymus Dna ◽

Suitable Model ◽

Principal Product ◽

Calf Thymus ◽

Sudan I ◽

A Minor

Ce(IV) ions in acidic medium convert a carcinogenic non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) into an ultimate carcinogen, which binds to calf thymus DNA. The principal product of Sudan I oxidation by the Ce(IV) system is the benzenediazonium ion. A minor product is the dihydroxyderivative of Sudan I, 1-(4-hydroxyphenylazo)-2,6-dihydroxynaphthalene. Other minor coloured products (yellow and brown) were not identified. The principal product (the benzenediazonium ion) is responsible for the carcinogenicity of Sudan I, as it covalently binds to DNA. Ce(IV) ions in acidic medium represent a suitable model system, which imitates the activation route of carcinogenic azo dyes.

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