Ce(IV)-mediated formation of benzenediazonium ion from a non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) and its binding to DNA

Ce(IV) ions in acidic medium convert a carcinogenic non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) into an ultimate carcinogen, which binds to calf thymus DNA. The principal product of Sudan I oxidation by the Ce(IV) system is the benzenediazonium ion. A minor product is the dihydroxyderivative of Sudan I, 1-(4-hydroxyphenylazo)-2,6-dihydroxynaphthalene. Other minor coloured products (yellow and brown) were not identified. The principal product (the benzenediazonium ion) is responsible for the carcinogenicity of Sudan I, as it covalently binds to DNA. Ce(IV) ions in acidic medium represent a suitable model system, which imitates the activation route of carcinogenic azo dyes.

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Fluorescence and Molecular Simulation Studies on the Interaction between Imidazolium-Based Ionic Liquids and Calf Thymus DNA

Processes ◽

10.3390/pr8010013 ◽

2019 ◽

Vol 8 (1) ◽

pp. 13

Author(s):

Khairulazhar Jumbri ◽

Mohd Azlan Kassim ◽

Normawati M. Yunus ◽

Mohd Basyaruddin Abdul Rahman ◽

Haslina Ahmad ◽

...

Keyword(s):

Ionic Liquids ◽

Double Helix ◽

Structural Features ◽

Docking Studies ◽

Calf Thymus Dna ◽

Valuable Insight ◽

Screening Model ◽

Calf Thymus ◽

A Minor ◽

Insight Into

This work presents a molecular level investigation on the nature and mode of binding between imidazolium-based ionic liquids (ILs) ([Cnbim]Br where n = 2, 4, 6) with calf thymus DNA. This investigation offers valuable insight into the mechanisms of interactions that can affect the structural features of DNA and possibly cause the alteration or inhibition of DNA function. To expedite analysis, the study resorted to using molecular docking and COnductor like Screening MOdel for Real Solvents (COSMO-RS) in conjunction with fluorescence spectroscopic data for confirmation and validation of computational results. Both the fluorescence and docking studies consistently revealed a weak interaction between the two molecules, which corresponded to the binding energy of a stable docking conformation in the range of −5.19 to −7.75 kcal mol−1. As predicted, the rod-like structure of imidazolium-based ILs prefers to bind to the double-helix DNA through a minor groove. Interestingly, the occurrence of T-shape π-π stacking was observed between the amine group in adenine that faces the aromatic ring of imidazole. In addition, data of COSMO-RS for the interaction of individual nucleic acid bases to imidazolium-based ILs affirmed that ILs showed a propensity to bind to different bases, the highest being guanine followed by cytosine, thymine, uracil, and adenine.

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Comparative Analysis of [Au(en)2]3+ and [Pt(en)2]2+ non Covalent Binding to Calf Thymus DNA

Metal-Based Drugs ◽

10.1155/mbd.2000.253 ◽

2000 ◽

Vol 7 (5) ◽

pp. 253-256 ◽

Cited By ~ 3

Author(s):

Giordana Marcon ◽

Tim O'Connell ◽

Pierluigi Orioli ◽

Luigi Messori

Keyword(s):

Comparative Analysis ◽

Electrostatic Interactions ◽

Covalent Binding ◽

Minor Groove ◽

Sodium Cyanide ◽

Calf Thymus Dna ◽

Minor Groove Binder ◽

Calf Thymus ◽

Dna Conformations ◽

A Minor

Reactions of the complexes bisethylendiammine gold(III) and bisethylendiammine platinum(II) with calfthymus DNA were comparatively analysed. Both complexes bind DNA non-covalently most probably on the basis of electrostatic interactions. Binding of either complex at low ratios results into modest modifications of B-type DNA conformations, as detected by CD. Far larger CD alterations are observed at high ratios. The gold(III) chromophore is scarcely perturbed by DNA addition Binding of [Au(en)2]Cl3 to calf thymus DNA is reversed by sodium cyanide. By analogy with the case of [Pt(en)2]Cl2 it is suggested that Auen acts as a minor groove binder.

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Drosophila melanogaster – a suitable model system to study epithelial immune responses in-vivo

Pneumologie ◽

10.1055/s-0035-1548648 ◽

2015 ◽

Vol 69 (04) ◽

Author(s):

C Wagner ◽

H Fehrenbach

Keyword(s):

Drosophila Melanogaster ◽

Immune Responses ◽

Model System ◽

Suitable Model

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Molecular Transformations in DNA under the Influence of UV-radiation

Current Applied Polymer Science ◽

10.2174/2452271604666200814123505 ◽

2020 ◽

Vol 04 ◽

Author(s):

Vigen G. Barkhudaryan ◽

Gayane V. Ananyan ◽

Nelli H. Karapetyan

Keyword(s):

Uv Radiation ◽

Uv Irradiation ◽

Absorption Spectroscopy ◽

Calf Thymus Dna ◽

Dilute Solutions ◽

Concentrated Solutions ◽

Buffer Solution ◽

Low Concentration ◽

Calf Thymus ◽

Dna Solutions

Background: The processes of destruction and crosslinking of macromolecules occur simultaneously under the influence of ultraviolet (UV) radiation in synthetic polymers, dry DNA and their concentrated solutions. Objective: The effect of UV radiation on calf thymus DNA in dilute solutions subjected to UV- irradiation was studied in this work. Method: The calf thymus DNA was studied in dilute solutions using viscometry, absorption spectroscopy and electrophoresis. Results: It was shown, that at a low concentration of DNA in the buffer solution ([DNA] = 85 μg / ml) under the influence of UV radiation, the processes of destruction of macromolecules and an increase in their flexibility predominate, which is accompanied by a gradual decrease in the viscosity of their solution. In addition, due to the low concentration of the solution, intramolecular crosslinking of macromolecules predominates, which also reduces their size and, consequently, the viscosity of the solution. Conclusion: It was concluded, that in dilute DNA solutions, due to the predominance of the processes of intramolecular crosslinking of macromolecules over intermolecular, only constant processes of decreasing the sizes of DNA macromolecules occur. As a result, its solubility remains virtually unchanged during UV irradiation. The described comments are also excellently confirmed by the results of absorption spectroscopy and electrophoresis

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