A simple and convenient Choline Oxidase inhibition based colorimetric biosensor for detection of organophosphorus class of pesticides

In this study the transgenic lines (TLs) of tobacco (Nicotianatabacum L.), which overexpress the heterologous gene encoding the bacterial enzyme choline oxidase were evaluated. The goal of our work is to study the effect of choline oxidase gene expression on the sensitivity of plant tissues to the action of NaCl. The regenerative capacity, rhizogenesis, the amount of photosynthetic pigments and osmotically active compounds (proline and glycine betaine) were assessed by in vitro cell culture methods using biochemical and morphological parameters. Transgenic lines with confirmed expression were characterized by high regeneration capacity from callus in the presence of 200 mmol NaCl, partial retention of viability at 400 mmol NaCl. These data correlated with the implicit response of regenerants and whole plants to the harmful effects of salinity. They turned out to be less sensitive to the presence of 200 mmol NaCl in the cultivation medium, in contrast to the WT plants.

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Design, Synthesis, Biological Evaluation of 3,5-diaryl-4,5-dihydro-1H-pyrazole carbaldehydes as Non-purine Xanthine Oxidase Inhibitors: Tracing the Anticancer Mechanism via Xanthine Oxidase Inhibition

Bioorganic Chemistry ◽

10.1016/j.bioorg.2020.104620 ◽

2021 ◽

pp. 104620

Author(s):

Gaurav Joshi ◽

Manisha Sharma ◽

Sourav Kalra ◽

Navnath S. Gavande ◽

Sandeep Singh ◽

...

Keyword(s):

Xanthine Oxidase ◽

Biological Evaluation ◽

Design Synthesis ◽

Xanthine Oxidase Inhibitors ◽

Anticancer Mechanism ◽

Oxidase Inhibition ◽

Xanthine Oxidase Inhibition

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Highly Sensitive Fluorescent Detection of Acetylcholine Based on the Enhanced Peroxidase-Like Activity of Histidine Coated Magnetic Nanoparticles

Nanomaterials ◽

10.3390/nano11051207 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1207

Author(s):

Hong Jae Cheon ◽

Quynh Huong Nguyen ◽

Moon Il Kim

Keyword(s):

Magnetic Nanoparticles ◽

High Sensitivity ◽

High Specificity ◽

Choline Oxidase ◽

Fluorescent Detection ◽

One Pot ◽

Site Structure ◽

Specificity And Sensitivity ◽

Highly Sensitive ◽

Peroxidase Mimics

Inspired by the active site structure of natural horseradish peroxidase having iron as a pivotal element with coordinated histidine residues, we have developed histidine coated magnetic nanoparticles (His@MNPs) with relatively uniform and small sizes (less than 10 nm) through one-pot heat treatment. In comparison to pristine MNPs and other amino acid coated MNPs, His@MNPs exhibited a considerably enhanced peroxidase-imitating activity, approaching 10-fold higher in catalytic reactions. With the high activity, His@MNPs then were exploited to detect the important neurotransmitter acetylcholine. By coupling choline oxidase and acetylcholine esterase with His@MNPs as peroxidase mimics, target choline and acetylcholine were successfully detected via fluorescent mode with high specificity and sensitivity with the limits of detection down to 200 and 100 nM, respectively. The diagnostic capability of the method is demonstrated by analyzing acetylcholine in human blood serum. This study thus demonstrates the potential of utilizing His@MNPs as peroxidase-mimicking nanozymes for detecting important biological and clinical targets with high sensitivity and reliability.

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Endothelial-to-mesenchymal transition in lipopolysaccharide-induced acute lung injury drives a progenitor cell-like phenotype

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.00074.2016 ◽

2016 ◽

Vol 310 (11) ◽

pp. L1185-L1198 ◽

Cited By ~ 12

Author(s):

Toshio Suzuki ◽

Yuji Tada ◽

Rintaro Nishimura ◽

Takeshi Kawasaki ◽

Ayumi Sekine ◽

...

Keyword(s):

Bone Marrow ◽

Endothelial Cells ◽

Acute Lung Injury ◽

Lung Injury ◽

Vascular Endothelial Cells ◽

Repair Process ◽

Vascular Endothelial ◽

Mesenchymal Transition ◽

Oxidase Inhibition ◽

Endothelial To Mesenchymal Transition

Pulmonary vascular endothelial function may be impaired by oxidative stress in endotoxemia-derived acute lung injury. Growing evidence suggests that endothelial-to-mesenchymal transition (EndMT) could play a pivotal role in various respiratory diseases; however, it remains unclear whether EndMT participates in the injury/repair process of septic acute lung injury. Here, we analyzed lipopolysaccharide (LPS)-treated mice whose total number of pulmonary vascular endothelial cells (PVECs) transiently decreased after production of reactive oxygen species (ROS), while the population of EndMT-PVECs significantly increased. NAD(P)H oxidase inhibition suppressed EndMT of PVECs. Most EndMT-PVECs derived from tissue-resident cells, not from bone marrow, as assessed by mice with chimeric bone marrow. Bromodeoxyuridine-incorporation assays revealed higher proliferation of capillary EndMT-PVECs. In addition, EndMT-PVECs strongly expressed c- kit and CD133. LPS loading to human lung microvascular endothelial cells (HMVEC-Ls) induced reversible EndMT, as evidenced by phenotypic recovery observed after removal of LPS. LPS-induced EndMT-HMVEC-Ls had increased vasculogenic ability, aldehyde dehydrogenase activity, and expression of drug resistance genes, which are also fundamental properties of progenitor cells. Taken together, our results demonstrate that LPS induces EndMT of tissue-resident PVECs during the early phase of acute lung injury, partly mediated by ROS, contributing to increased proliferation of PVECs.

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