Melatonin ameliorates bisphenol A-induced DNA damage in the germ cells of adult male rats

Various adverse effects of endocrine disruptors on the reproductive organs of male animals have been reported. We found that UDP-glucuronosyltransferase (UGT) activities towards bisphenol A, testosterone and oestradiol were significantly decreased in liver microsomes prepared from adult male Wistar rats administered with the endocrine disruptor bisphenol A (1mg/2 days for 2 or 4 weeks). However, suppression of the transferase activities was not observed in female rats, even after bisphenol A treatment for 4 weeks. Diethylstilbestrol, which is well known as an endocrine disruptor, had the same effects, but p-cumylphenol had no effect on UGT activities towards sex hormones. Co-administration of an anti-oestrogen, tamoxifen, inhibited the suppression of the transferase activities by bisphenol A. Western blotting analysis showed that the amount of UGT2B1, an isoform of UGT which glucuronidates bisphenol A, was decreased in the rat liver microsomes by the treatment. Northern blotting analysis also indicated that UGT2B1 mRNA in the liver was decreased by bisphenol A treatment. The suppression of UGT activities, UGT2B1 protein and UGT2B1 mRNA expression did not occur in female rats. The results indicate that bisphenol A treatment reduces the mRNA expression of UGT2B1 and other UGT isoforms that mediate the glucuronidation of sex hormones in adult male rats, and this suggests that the endocrine balance may be disrupted by suppression of glucuronidation.

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Assessment the Genotoxic Potential of Fluoxetine and Amitriptyline at Maximum Therapeutic Doses for Four-Week Treatment in Experimental Male Rats

Iraqi Journal of Pharmaceutical Sciences ( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512) ◽

10.31351/vol30iss1pp81-90 ◽

2021 ◽

Vol 30 (1) ◽

pp. 81-90

Author(s):

Imad A. Al-Obaidi ◽

Nada N. Al-Shawi

Keyword(s):

Bone Marrow ◽

Dna Damage ◽

Adult Male ◽

Negative Control Group ◽

Negative Control ◽

Male Rats ◽

Control Group ◽

Group Iii ◽

Group I ◽

Hydrochloride Solution

Abstract At any moment, the continuous usage of medications can accompanied by DNA damage and the accumulation of such damages can cause serious consequences. Antidepressants are long-term used drugs and the incidence of their genotoxic impacts cannot be excluded. Therefore, this work was designed to investigate the possible genotoxic effects of the commonly used antidepressants (fluoxetine and amitriptyline) in adult male rats. Detection of DNA damage in individual cells was assessed by comet and micronucleus assays in three different cell populations i.e. liver, testis and bone marrow tissues of 24 swiss albino adult male rats. The animals were randomly allocated into three groups of 8 rats each: Group I - rats orally-administered distilled water via gavage tube for four weeks as a negative control. Group II - rats orally-treated with fluoxetine hydrochloride solution (7.2mg/kg/day) via gavage tube for four weeks. Group III - rats orally-treated with amitriptyline hydrochloride solution (27mg/kg/day) via gavage tube for four weeks. The results showed that both drugs (Group II and Group III) induced the same extent of DNA damage, as evidenced by a significantly higher DNA fragmentation in liver and testis tissues with increased frequencies of micronuclei formation in bone marrow tissues as compared with the negative control (Group I). These findings indicates that both Fluoxetine and Amitriptyline have genotoxic potentials and can induce the same extent of cytogenetic damage in rats. Special precautions and medical supervision should be taken in consideration with their uses.

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The Therapeuticrole of Moringa Oleifera Against Bisphenol A toxicity That Induce Renal Effects in Rats

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/910/1/012084 ◽

2021 ◽

Vol 910 (1) ◽

pp. 012084

Author(s):

Hind Mohammed Saleh ◽

Hani Sabbar Ayed ◽

Ahmed Ibrahim Salih

Keyword(s):

Bisphenol A ◽

Adult Male ◽

Normal Saline ◽

Normal State ◽

Moringa Oleifera ◽

Male Rats ◽

Control Group ◽

Therapeutic Role ◽

Renal Effects

Abstract The objective of the current work was to induce histological lesions by BPA(Bisphenol A)and then diagnosis the therapeutic role of Moringa oleifera. 66 adult male rats were used in the present work and divided as following: Rats were administrated (orally) normal saline as control group. Rats group were administrated (orally) 5mg BPA and divided into 4 subgroups were each subgroup treated with Moringa oleifera (100mg/kg, 200mg/kg, 300mg/kg and 400mg/kg), respectively. Rats were administrated (orally) 10mg BPA and divided to 4 subgroups were each subgroup treated with Moringa oleifera (100mg/kg, 200mg/kg, 300mg/kg and 400mg/kg), respectively. The findings of BPA groups showed significant (P<0.05) elevated in urea and creatinine with different histological lesions in the kidney include damaged glomerulus, degeneration of tubules cells, and lymphocytes infiltration. After treatment with Moringa oleifera, renal parameters and kidney tissues were back to the normal state and non-significant (P≤0.05) changes compared with the control group.

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EFFECT OF BISPHENOL A ON THYROID, LIVER AND TESTICULAR FUNCTIONS IN ADULT MALE RATS

Basrah Journal of veterinary Research ◽

10.33762/bvetr.2015.102436 ◽

2015 ◽

Vol 14 (1) ◽

pp. 187-206 ◽

Cited By ~ 1

Author(s):

Jassim. M. A. Alkalby

Keyword(s):

Bisphenol A ◽

Adult Male ◽

Male Rats

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Induction of Apoptosis in the Germ Cells of Adult Male Rats after Exposure to Cyclophosphamide1

Biology of Reproduction ◽

10.1095/biolreprod56.6.1490 ◽

1997 ◽

Vol 56 (6) ◽

pp. 1490-1497 ◽

Cited By ~ 100

Author(s):

Lu Cai ◽

Barbara F. Hales ◽

Bernard Robaire

Keyword(s):

Germ Cells ◽

Adult Male ◽

Male Rats ◽

Induction Of Apoptosis

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Bisphenol-A impairs memory and reduces dendritic spine density in adult male rats.

Behavioral Neuroscience ◽

10.1037/a0025959 ◽

2012 ◽

Vol 126 (1) ◽

pp. 175-185 ◽

Cited By ~ 75

Author(s):

Tehila Eilam-Stock ◽

Peter Serrano ◽

Maya Frankfurt ◽

Victoria Luine

Keyword(s):

Bisphenol A ◽

Adult Male ◽

Dendritic Spine ◽

Male Rats ◽

Spine Density ◽

Dendritic Spine Density

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Effects of Sub-Chronic Exposure to Imidacloprid on Reproductive Organs of Adult Male Rats: Antioxidant State, DNA Damage, and Levels of Essential Elements

Antioxidants ◽

10.3390/antiox10121965 ◽

2021 ◽

Vol 10 (12) ◽

pp. 1965

Author(s):

Blanka Tariba Lovaković ◽

Vilena Kašuba ◽

Ankica Sekovanić ◽

Tatjana Orct ◽

Antonija Jančec ◽

...

Keyword(s):

Dna Damage ◽

Adult Male ◽

Biological Effects ◽

Good Alternative ◽

Reproductive Organs ◽

Negative Control ◽

Essential Elements ◽

Male Rats ◽

Testis Weight ◽

Low Doses

Although considered a good alternative to organophosphate pesticides, there are reports indicating adverse effects of neonicotinoid insecticides on reproduction. Our aim was to assess the effects of exposure to low doses of imidacloprid on antioxidant state, DNA damage, and concentration of essential elements in the testes and epididymis using a rat model. Adult male Wistar rats were orally treated with doses comparable to currently proposed health-based reference values: 0.06 (ADI), 0.80 (10× AOEL), or 2.25 (1/200 LD50) mg/kg b.w./day for 28 consecutive days. Exposure to 2.25 mg/kg b.w./day of imidacloprid resulted in a significantly lower testis weight (1.30 ± 0.17 g compared to 1.63 ± 0.15 g in controls). Treatment with 0.06 mg/kg b.w./day increased the level of reduced glutathione in the epididymis (73%), while the activities of epididymal glutathione peroxidase and superoxide dismutase significantly increased in all treated rats (74–92% and 26–39%, respectively). Exposure to imidacloprid resulted in a low, but significant, level of DNA damage in testicular sperm cells regardless of the concentration applied (<28% compared to the negative control). Higher concentrations of Mo were measured in the testes of rats treated with 0.80 and 2.25 mg/kg b.w./day (72.9 ± 7.9 and 73.9 ± 9.1 mg/g, respectively) compared to the control animals (60.5 ± 7.8 mg/g). Higher concentrations of Na were measured in the testes of rats treated with 2.25 mg/kg b.w./day (1679 ± 82 mg/g compared to 1562 ± 56 mg/g in controls). The fact that such low doses of imidacloprid were able to produce measurable biological effects calls for the further evaluation of this widely used insecticide.

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