Gypsum-related compensation of ions uptake by highly porous hydroxyapatite ceramics – Consequences for osteoblasts growth and proliferation

2022 ◽  
pp. 112665
Author(s):  
Justyna Zalewska ◽  
Agata Przekora ◽  
Krzysztof Pałka ◽  
Anna Belcarz
Keyword(s):  
Porous Hydroxyapatite ◽  
Hydroxyapatite Ceramics ◽  
Highly Porous

Highly Porous Hydroxyapatite Ceramics for Engineering Applicatios

2010 ◽  
Vol 63 ◽  
pp. 408-413 ◽  
Author(s):  
Hrvoje Ivankovic ◽  
Sebastijan Orlic ◽  
Dajana Kranzelic ◽  
Emilija Tkalcec
Keyword(s):  
Average Length ◽  
Average Diameter ◽  
Ftir Analysis ◽  
X Ray Diffraction ◽  
Gas Cell ◽  
Porous Hydroxyapatite ◽  
Hydroxyapatite Ceramics ◽  
Heat Treated ◽  
Hydrothermal Transformation ◽  
Highly Porous

Highly porous hydroxyapatite (Ca10(PO4)6(OH)2, HA) was prepared through hydrothermal (HT) transformation of aragonitic cuttlefish bones (Seppia Officinalis L. Adriatic Sea) in the temperature range from 140°C to 220°C for 20 minutes to 48 hours. Mechanism of hydrothermal transformation of bones was investigated by DTA/TG analyzer coupled online with FTIR spectrometric gas cell equipment (DTA-TG-EGA-FTIR analysis), X-ray diffraction analysis (XRD) and scanning electron microscopy (SEM). DTA-TG-EGA-FTIR analysis have shown the release of CO2 at about 400°C, 680°C and 990°C. The first release could be attributed to organics not completely removed from the heat treated bones, and the second release to decomposition of unconverted aragonite, whereas, the third one could be attributed to CO3 2– groups incorporated in the structure of HA. The interconnecting porous morphology of the starting material (aragonite) was maintained during the HT treatment. The formation of dandelion-like HA spheres with diameter from 3 to 8 μm were observed, which further transformed into nanoplates and nanorods with an average diameter of about 200-300 nm and an average length of about 8-10 μm.

Preparation of Highly Porous Hydroxyapatite Ceramics from Cuttlefish Bone

2006 ◽  
Vol 49 ◽  
pp. 142-147 ◽  
Author(s):  
H. Ivankovic ◽  
G. Gallego Ferrer ◽  
E. Tkalcec ◽  
M. Ivankovic
Keyword(s):  
Tissue Engineering ◽  
Specific Surface ◽  
Pore Volume ◽  
Engineering Application ◽  
Hollow Structure ◽  
Total Pore Volume ◽  
Tissue Engineering Application ◽  
Porous Hydroxyapatite ◽  
Hydroxyapatite Ceramics ◽  
Highly Porous

Scaffold of hydroxyapatite for further tissue-engineering application was produced by hydrothermal treatment of cuttlefish bone originated aragonite at 200°C. Aragonite (CaCO3) monoliths were completely transformed into hydroxyapatite after 48 hours of HT treatment. The substitution of CO3- groups predominantly into the PO4 3- sites of the Ca10(PO4)6(OH)2 structure was suggested by FTIR spectroscopy. SEM micrographs have shown that the interconnected hollow structure with pillars connecting parallel lamellae in cuttlefish bone is maintained after conversion. Specific surface area (SBET) and total pore volume increased and mean pore size decreased by HT treatment.

scholarly journals Rapid conversion of highly porous borate glass microspheres into hydroxyapatite

2021 ◽  
Author(s):  
Md Towhidul Islam ◽  
Laura Macri-Pellizzeri ◽  
Virginie Sottile ◽  
Ifty Ahmed
Keyword(s):  
Borate Glass ◽  
Rapid Development ◽  
Glass Microspheres ◽  
Porous Hydroxyapatite ◽  
Interconnected Porosity ◽  
Highly Porous ◽  
Rapid Conversion

This paper reports on the rapid development of porous hydroxyapatite (HA) microspheres with large external pores and fully interconnected porosity.

Preparation of a Hydroxyapatite Ceramic with Comblike Tubules Structure and its Permeablity

2018 ◽  
Vol 768 ◽  
pp. 135-139 ◽  
Author(s):  
Xin Yi Zhao ◽  
Xiao Peng Li ◽  
Kang Zhao ◽  
Yu Fei Tang
Keyword(s):  
Cross Section ◽  
Longitudinal Section ◽  
Hydraulic Conductance ◽  
Hydroxyapatite Ceramic ◽  
Porous Hydroxyapatite ◽  
Dentin Tubule ◽  
Hydroxyapatite Powder ◽  
Hydroxyapatite Ceramics ◽  
Porous Hydroxyapatite Ceramic ◽  
C 30

The objectives of this study are to prepare a porous hydroxyapatite ceramic which has dentin tubule-like structure and determine its permeability. Slurry of hydroxyapatite powder, deionized water and a binder was poured into a ring which was placed on a freezing plate.The hydroxyapatite slurry was freezed in a certain rate (by controlling the temperature of the freeze plate at −15°C, −30°C and −45°C) for a certain period of time, then the freezed sample was freezing dried to remove the frozen vehicle, followed by being sintered at 1250 °C for 2 h. After that，the morphology of the cross section and longitudinal section of the sintered porous hydroxyapatite ceramic was observed by SEM and the hydraulic conductance of cross section discs of the sintered porous hydroxyapatite were determined using a self-made micro-flowing permeability tester. Results showed that the prepared hydroxyapatite ceramics having bottom-up unidirectional comblike tubule structure and the tubule diameters associated with the temperature of freezing plate.The ceramic discs prepared on the freezing plate of −45°C exhibited similarity to nature dentin tubule, with a diameter of 9.72±3.41mm and a hydraulic conductance of 0.16±0.09 ml×min-1×cm-2×cm×H2O-1.

C-src oncogene mRNA expression in porous hydroxyapatite ceramics

Bioceramics ◽  
1997 ◽  
pp. 595-598
Author(s):  
K. Mishima ◽  
H. Ohgushi ◽  
T. Yoshikawa ◽  
H. Nakajima ◽  
E. Yamada ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Porous Hydroxyapatite ◽  
Hydroxyapatite Ceramics

Effect of the Pore Structure of an Apatite-Fiber Scaffold on the Differentiation of P19.CL6 Cells into Cardiomyocytes

2018 ◽  
Vol 782 ◽  
pp. 116-123
Author(s):  
Yuzuha Ichikawa ◽  
Kei Yasuda ◽  
Masahiro Takahara ◽  
Mamoru Aizawa ◽  
Nobuyuki Kanzawa
Keyword(s):  
Troponin T ◽  
Specific Gene ◽  
Porous Hydroxyapatite ◽  
Spherical Carbon ◽  
Functional Maturation ◽  
Biomedical Device ◽  
Hydroxyapatite Ceramics ◽  
Proliferation And Differentiation ◽  
Device Applications ◽  
Radial Flow Bioreactor

We previously reported that P19.CL6 cells can be cultured in porous hydroxyapatite ceramics prepared by firing green compacts consisting of apatite fibers and spherical carbon beads (150 μm in diameter). Cells cultured for 20 days in an apatite-fiber scaffold (AFS) proliferated and differentiated into cells expressing troponin T, a cardiomyocyte-specific gene, but the expression level was insufficient to support the functional maturation of cells required for biomedical device applications. In this study, we aimed to optimize the internal AFS environment for cardiomyocytes by mixing two sizes (150-and 20-μm) of carbon beads. P19.CL6 cells were cultured in AFS materials comprising different carbon ratios in the presence of alpha-MEM with (AFS+) or without (AFS-) dimethyl sulfoxide (DMSO), and cell growth and gene expression were assessed. We found that AFS(50, 1:1 ratio) is the most suitable scaffold for the proliferation and differentiation of P19.CL6 cells and the addition of DMSO to the culture medium is necessary for differentiation into cardiomyocytes. We also assessed the culture of P19.CL6 cells in AFS in a radial-flow bioreactor for several days.

Electrovector Effect of Polarized Porous Hydroxyapatite Ceramics

2006 ◽  
pp. 1043-1046
Author(s):  
Chikako Ikeda ◽  
Mikihiro Ueki ◽  
Satoshi Nakamura ◽  
Takayuki Kobayashi ◽  
Kimihiro Yamashita
Keyword(s):  
Porous Hydroxyapatite ◽  
Hydroxyapatite Ceramics
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