State-of-the-art nanotechnologies for the detection, recovery, analysis and elimination of liquid biopsy components in cancer

Liquid biopsy via isolation of circulating tumour cells (CTCs) represents a promising diagnostic tool capable of supplementing state-of-the-art for prostate cancer (PC) prognosis. Unfortunately, most of CTC technologies, such as AdnaTest or Cellsearch, critically rely on the Epithelial-Cell-Adhesion-Molecule (EpCAM) marker, limiting the possibility of detecting stem-like cells (CSCs) and mesenchymal-like cells (EMT-CTCs) that are present during PC progression. In this tontext, dielectrophoresis (DEP) is an epCAM independent, label-free, enrichment system, separating rare cells simply on the basis of their specific electrical properties. As compared to other technollgies, DEP represents a superior technique in terms of running costs, cells yield and specificity, but due to its higher complexity, requires still further technical as well as clinical development. Interestingly, DEP can be improved by the use of microfluid, nanostructured materials and fluoroimaging in order to increase its potential applications. In the context of PC, the utility of DEP can be translated in its capacity to detect CTC in the bloodstream in their epithelial, mesenchymal, or epithelial-mesenchymal phenotypes, which should be taken into account when choosing CTC enrichment and analysis methods for PC prognosis and early diagnosis.

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Exosomes as Liquid Biopsy Biomarkers in Diffuse Large B-cell Lymphoma (DLBCL)- Current State-of-the-Art and Unmet Clinical Needs

10.22541/au.159050477.74611924 ◽

2020 ◽

Author(s):

Kenneth Ofori ◽

Govind Bhagat ◽

Alex Rai

Keyword(s):

B Cell ◽

Liquid Biopsy ◽

Cell Lymphoma ◽

State Of The Art ◽

B Cell Lymphoma ◽

Current State ◽

Large B Cell Lymphoma ◽

Large B Cell

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Using gold nanoparticles to detect single-nucleotide polymorphisms: toward liquid biopsy

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.11.20 ◽

2020 ◽

Vol 11 ◽

pp. 263-284 ◽

Cited By ~ 1

Author(s):

María Sanromán Iglesias ◽

Marek Grzelczak

Keyword(s):

Gold Nanoparticles ◽

Liquid Biopsy ◽

Materials Science ◽

State Of The Art ◽

Point Of Care ◽

Enzymatic Reactions ◽

Nucleotide Polymorphisms ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Science Community

The possibility of detecting genetic mutations rapidly in physiological media through liquid biopsy has attracted the attention within the materials science community. The physical properties of nanoparticles combined with robust transduction methods ensure an improved sensitivity and specificity of a given assay and its implementation into point-of-care devices for common use. Covering the last twenty years, this review gives an overview of the state-of-the-art of the research on the use of gold nanoparticles in the development of colorimetric biosensors for the detection of single-nucleotide polymorphism as cancer biomarker. We discuss the main mechanisms of the assays that either are assisted by DNA-based molecular machines or by enzymatic reactions, summarize their performance and provide an outlook towards future developments.

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Practical Picture Processing

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051700 ◽

1974 ◽

Vol 32 ◽

pp. 338-339

Author(s):

T. A. Welton

Keyword(s):

Radiation Damage ◽

Coherence Length ◽

Spatial Information ◽

State Of The Art ◽

Coherent Radiation ◽

The State ◽

Energy Spread ◽

Electron Micrograph ◽

Picture Processing ◽

Molecular Skeleton

Various authors have emphasized the spatial information resident in an electron micrograph taken with adequately coherent radiation. In view of the completion of at least one such instrument, this opportunity is taken to summarize the state of the art of processing such micrographs. We use the usual symbols for the aberration coefficients, and supplement these with £ and 6 for the transverse coherence length and the fractional energy spread respectively. He also assume a weak, biologically interesting sample, with principal interest lying in the molecular skeleton remaining after obvious hydrogen loss and other radiation damage has occurred.

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A Macintosh Interface for the Cameca Camebax-Micro Electron Microprobe

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010018094x ◽

1990 ◽

Vol 48 (1) ◽

pp. 438-439

Author(s):

Carl E. Henderson

Keyword(s):

Electron Microprobe ◽

Processing Speed ◽

Word Processing ◽

State Of The Art ◽

Computer Control ◽

Third Party ◽

Disk Storage ◽

The Past ◽

User Facility ◽

Instrument Control

Over the past few years it has become apparent in our multi-user facility that the computer system and software supplied in 1985 with our CAMECA CAMEBAX-MICRO electron microprobe analyzer has the greatest potential for improvement and updating of any component of the instrument. While the standard CAMECA software running on a DEC PDP-11/23+ computer under the RSX-11M operating system can perform almost any task required of the instrument, the commands are not always intuitive and can be difficult to remember for the casual user (of which our laboratory has many). Given the widespread and growing use of other microcomputers (such as PC’s and Macintoshes) by users of the microprobe, the PDP has become the “oddball” and has also fallen behind the state-of-the-art in terms of processing speed and disk storage capabilities. Upgrade paths within products available from DEC are considered to be too expensive for the benefits received. After using a Macintosh for other tasks in the laboratory, such as instrument use and billing records, word processing, and graphics display, its unique and “friendly” user interface suggested an easier-to-use system for computer control of the electron microprobe automation. Specifically a Macintosh IIx was chosen for its capacity for third-party add-on cards used in instrument control.

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