Magnetic separation on a new level: first cGMP compliant magnetic separation device, cleaning validation studies and protein purification optimization

2016 ◽  
Vol 33 ◽  
pp. S27-S28 ◽  
Author(s):  
Moritz Ebeler ◽  
Matthias Franzreb
Keyword(s):  
Protein Purification ◽  
Magnetic Separation ◽  
Validation Studies ◽  
Separation Device ◽  
Cleaning Validation

An Automated Magnetic Separation Device Coupled with a Fluorescent Biosensor for Detection of Antibiotic Residues

2021 ◽  
Vol 64 (1) ◽  
pp. 23-30
Author(s):  
Aoming Liang ◽  
Yafang Shen ◽  
Yawen He ◽  
Jianping Wang ◽  
Yanbin Li
Keyword(s):  
Magnetic Separation ◽  
Rapid Detection ◽  
Magnetic Beads ◽  
Limit Of Detection ◽  
Sample Pretreatment ◽  
List Type ◽  
Antibiotic Residues ◽  
Separation Device ◽  
Fluorescent Biosensor ◽  
Automated Separation

HighlightsA practical magnetic separation device was designed, fabricated, and evaluated for enrofloxacin detection.Coupled with a fluorescent biosensor, the device could automatically process a sample in 50 min.The device performed incubation and magnetic separation using a pipette method.The device has the advantages of low-cost and feasibility for on-site detection.Abstract. Antibiotic residues have been a continuing concern in food safety, raising a great issue in human health. For rapid detection of antibiotics, an automated device was developed that can capture and separate a target analyte based on immunomagnetic beads. This automated separation device is suitable for separating the magnetic beads in a preprocessing step, with liquid transfer and magnetic enrichment functions. The device was combined with a fluorescent biosensor to simplify the cumbersome pretreatment of enrofloxacin. In our experiments, enrofloxacin in water samples was used as the detection object, and the entire process could be completed in less than 50 min with automated operation. The lower limit of detection reached 54 ng mL-1 (S/N = 3). The fluorescent biosensor has been enhanced with this automated separation device for more sensitive rapid detection of antibiotic residues in the food supply chain and environment. Keywords: Antibiotic detection, Automation, Fluorescent biosensor, Immunomagnetic separation, Sample pretreatment.

Cleaning Validation Studies for Multi-Purpose Facility : Vial Filling Machine

2009 ◽  
Vol 39 (4) ◽  
pp. 263-267
Author(s):  
Han-Gon Choi ◽  
Ho-Joon Yang ◽  
Young-Ran Kim ◽  
Jun-Ho Sung ◽  
Ma-Ro Hwang ◽  
...  
Keyword(s):  
Validation Studies ◽  
Cleaning Validation

scholarly journals Development of a High-Throughput Magnetic Separation Device for Malaria-Infected Erythrocytes

2017 ◽  
Vol 45 (12) ◽  
pp. 2888-2898 ◽  
Author(s):  
A. Blue Martin ◽  
Wei-Tao Wu ◽  
Marina V. Kameneva ◽  
James F. Antaki
Keyword(s):  
Magnetic Separation ◽  
High Throughput ◽  
Separation Device

scholarly journals Affinity sedimentation and magnetic separation with plant-made immunosorbent nanoparticles for therapeutic protein purification

2021 ◽  
Author(s):  
Matthew J McNulty ◽  
Anton Schwartz ◽  
Jesse Delzio ◽  
Kalimuthu Karuppanan ◽  
Aaron Jacobson ◽  
...  
Keyword(s):  
Protein Purification ◽  
Magnetic Separation ◽  
Magnetic Particles ◽  
Binding Capacity ◽  
Therapeutic Protein ◽  
Vector System ◽  
High Yield ◽  
Affinity Capture ◽  
Processing Scheme ◽  
Industry Standards

The virus-based immunosorbent nanoparticle is a nascent technology being developed to serve as a simple and efficacious agent in biosensing and therapeutic antibody purification. There has been particular emphasis on the use of plant virions as immunosorbent nanoparticle chassis for their diverse morphologies and accessible, high yield manufacturing via crop cultivation. To date, studies in this area have focused on proof-of-concept immunosorbent functionality in biosensing and purification contexts. Here we consolidate a previously reported pro-vector system into a single Agrobacterium tumefaciens vector to investigate and expand the utility of virus-based immunosorbent nanoparticle technology for therapeutic protein purification. We demonstrate the use of this technology for Fc-fusion protein purification, characterize key nanomaterial properties including binding capacity, stability, reusability, and particle integrity, and present an optimized processing scheme with reduced complexity and increased purity. Furthermore, we present a coupling of virus-based immunosorbent nanoparticles with magnetic particles as a strategy to overcome limitations of the immunosorbent nanoparticle sedimentation-based affinity capture methodology. We report magnetic separation results which exceed the binding capacity of current industry standards by an order of magnitude.

High Performance Microfluidic-Based DNA Isolation Chip

2013 ◽  
Author(s):  
Jeff Darabi
Keyword(s):  
Magnetic Separation ◽  
High Performance ◽  
Magnetic Beads ◽  
Biological Entity ◽  
Separation Device ◽  
Separation Systems ◽  
Multiple Sample ◽  
Downstream Analysis ◽  
Biological Entities ◽  
The Magnetic Field

Magnetic separation is one of the effective ways to separate specific biological entities such as DNA/RNA, bacteria, and cells from their native environment for subsequent downstream analysis. The process involves the labeling of the desired biological entity with magnetic beads followed by separating the tagged entities via a magnetic separation device. In conventional tube-based magnetic separation, magnetically labeled biological entities are retained on the inner wall of the tube by applying an external magnet, while the supernatant is decanted off. Removing the tube from the magnetic field enables resuspension of the target entity. Although widely used, there are limitations to the conventional magnetic separation method. For example, there is a significant sample loss due to multiple sample handling, washing, and transfer. In addition, manual magnetic separation systems are labor intensive and their effectiveness is user-dependent.

Magnetic separation of iron and heavy metals from water

2003 ◽  
Vol 47 (1) ◽  
pp. 29-32 ◽  
Author(s):  
J.D. Navratil ◽  
M.T. Shing Tsair
Keyword(s):  
Heavy Metals ◽  
Magnetic Separation ◽  
Experimental Studies ◽  
Sorption Process ◽  
Separation Device ◽  
Nanoscale Particles ◽  
Iron Ferrite ◽  
Magnetic Filtration ◽  
Sorption Mechanisms ◽  
Water Passage

A magnetic separation device is being developed for removal of iron and heavy metals from water. The device consists of a column of supported magnetite surrounded by a movable permanent magnet. The mineral magnetite, or synthetically prepared iron ferrite (FeO.Fe2O3), is typically supported on various materials to permit adequate water passage through the column. In the presence of an external magnetic field, enhanced capacity was observed in using supported magnetite for removal of actinides and heavy metals from wastewater. The enhanced capacity is primarily due to magnetic filtration of colloidal and nanoscale particles along with some complex and ion exchange sorption mechanisms. This paper will review some previous work on the use of magnetite for wastewater treatment and discuss the development and potential of the magnetic nanoscale filtration/sorption process for water treatment. Recent research results are also presented on preliminary experimental studies using the process with water samples containing iron.

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