High Performance Microfluidic-Based DNA Isolation Chip

2013 ◽  
Author(s):  
Jeff Darabi
Keyword(s):  
Magnetic Separation ◽  
High Performance ◽  
Magnetic Beads ◽  
Biological Entity ◽  
Separation Device ◽  
Separation Systems ◽  
Multiple Sample ◽  
Downstream Analysis ◽  
Biological Entities ◽  
The Magnetic Field

Magnetic separation is one of the effective ways to separate specific biological entities such as DNA/RNA, bacteria, and cells from their native environment for subsequent downstream analysis. The process involves the labeling of the desired biological entity with magnetic beads followed by separating the tagged entities via a magnetic separation device. In conventional tube-based magnetic separation, magnetically labeled biological entities are retained on the inner wall of the tube by applying an external magnet, while the supernatant is decanted off. Removing the tube from the magnetic field enables resuspension of the target entity. Although widely used, there are limitations to the conventional magnetic separation method. For example, there is a significant sample loss due to multiple sample handling, washing, and transfer. In addition, manual magnetic separation systems are labor intensive and their effectiveness is user-dependent.

An Automated Magnetic Separation Device Coupled with a Fluorescent Biosensor for Detection of Antibiotic Residues

2021 ◽  
Vol 64 (1) ◽  
pp. 23-30
Author(s):  
Aoming Liang ◽  
Yafang Shen ◽  
Yawen He ◽  
Jianping Wang ◽  
Yanbin Li
Keyword(s):  
Magnetic Separation ◽  
Rapid Detection ◽  
Magnetic Beads ◽  
Limit Of Detection ◽  
Sample Pretreatment ◽  
List Type ◽  
Antibiotic Residues ◽  
Separation Device ◽  
Fluorescent Biosensor ◽  
Automated Separation

HighlightsA practical magnetic separation device was designed, fabricated, and evaluated for enrofloxacin detection.Coupled with a fluorescent biosensor, the device could automatically process a sample in 50 min.The device performed incubation and magnetic separation using a pipette method.The device has the advantages of low-cost and feasibility for on-site detection.Abstract. Antibiotic residues have been a continuing concern in food safety, raising a great issue in human health. For rapid detection of antibiotics, an automated device was developed that can capture and separate a target analyte based on immunomagnetic beads. This automated separation device is suitable for separating the magnetic beads in a preprocessing step, with liquid transfer and magnetic enrichment functions. The device was combined with a fluorescent biosensor to simplify the cumbersome pretreatment of enrofloxacin. In our experiments, enrofloxacin in water samples was used as the detection object, and the entire process could be completed in less than 50 min with automated operation. The lower limit of detection reached 54 ng mL-1 (S/N = 3). The fluorescent biosensor has been enhanced with this automated separation device for more sensitive rapid detection of antibiotic residues in the food supply chain and environment. Keywords: Antibiotic detection, Automation, Fluorescent biosensor, Immunomagnetic separation, Sample pretreatment.

A feasibility study of magnetic separation of magnetic nanoparticle for forward osmosis

2011 ◽  
Vol 64 (2) ◽  
pp. 469-476 ◽  
Author(s):  
Y. C. Kim ◽  
S. Han ◽  
S. Hong
Keyword(s):  
Magnetic Field ◽  
Magnetic Separation ◽  
High Performance ◽  
Magnetic Nanoparticle ◽  
Large Scale ◽  
Magnetic Field Gradient ◽  
Forward Osmosis ◽  
Magnetic Separator ◽  
High Gradient Magnetic Separation ◽  
The Magnetic Field

It was recently reported that a UK company has developed a naturally non-toxic magnetoferritin to act as a draw solute for drawing water in forward osmosis process. The gist of this technology is the utilization of the magnetic nanoparticle and high-gradient magnetic separation for draw solute separation and reuse. However, any demonstration on this technology has not been reported yet. In this study, a feasibility test of magnetic separation using magnetic nanoparticle was therefore performed to investigate the possibility of magnetic separation in water treatment such as desalination. Basically, a magnetic separation system consisted of a column packed with a bed of magnetically susceptible wools placed between the poles of electromagnet and Fe3O4 magnetic nanoparticle was used as a model nanoparticle. The effect of nanoparticle size to applied magnetic field in separation column was experimentally investigated and the magnetic field distribution in a magnet gap and the magnetic field gradient around stainless steel wool wire were analyzed through numerical simulation. The amount of magnetic nanoparticle captured in the separator column increased as the magnetic field strength and particle size increased. As a result, if magnetic separation is intended to be used for draw solute separation and reuse, both novel nanoparticle and large-scale high performance magnetic separator must be developed.

scholarly journals Simulation study of the plasma-brake effect

2014 ◽  
Vol 32 (10) ◽  
pp. 1207-1216 ◽  
Author(s):  
P. Janhunen
Keyword(s):  
Magnetic Field ◽  
High Performance ◽  
Ionospheric Plasma ◽  
Low Earth Orbit ◽  
Earth Orbit ◽  
Breaking Force ◽  
Field Orientation ◽  
Particle In Cell ◽  
Leo Satellite ◽  
The Magnetic Field

Abstract. Plasma brake is a thin, negatively biased tether that has been proposed as an efficient concept for deorbiting satellites and debris objects from low Earth orbit. We simulate the interaction with the ionospheric plasma ram flow with the plasma-brake tether by a high-performance electrostatic particle in cell code to evaluate the thrust. The tether is assumed to be perpendicular to the flow. We perform runs for different tether voltage, magnetic-field orientation and plasma-ion mass. We show that a simple analytical thrust formula reproduces most of the simulation results well. The interaction with the tether and the plasma flow is laminar (i.e. smooth and not turbulent) when the magnetic field is perpendicular to the tether and the flow. If the magnetic field is parallel to the tether, the behaviour is unstable and thrust is reduced by a modest factor. The case in which the magnetic field is aligned with the flow can also be unstable, but does not result in notable thrust reduction. We also correct an error in an earlier reference. According to the simulations, the predicted thrust of the plasma brake is large enough to make the method promising for low-Earth-orbit (LEO) satellite deorbiting. As a numerical example, we estimate that a 5 km long plasma-brake tether weighing 0.055 kg could produce 0.43 mN breaking force, which is enough to reduce the orbital altitude of a 260 kg object mass by 100 km over 1 year.

The Research of a New Method for Manipulating Magnetic Beads through Multi-Layered Flat Micro-Coils Coupled with Permanent Magnet

2013 ◽  
Vol 753-755 ◽  
pp. 1571-1575
Author(s):  
Zhi Hua Liu ◽  
Yu Feng Huang ◽  
Jian Peng Li ◽  
Xin Wei Xu
Keyword(s):  
Magnetic Field ◽  
Permanent Magnet ◽  
Flow Velocity ◽  
Magnetic Beads ◽  
Magnetic Bead ◽  
New Method ◽  
Viscous Resistance ◽  
Main Factors ◽  
The Magnetic Field

Magnetic bead droplet's non-contacted manipulation can be realized in Electromagnetic MEMS, but how to achieve magnetic beads manipulation is the major problem. A new method of multi-layered flat coils coupled with permanent magnet was proposed. Firstly, the theory of magnetic bead manipulation was analyzed and the main factors affected the magnetic beads manipulation was identified; then the magnetic field of multi-layered flat coils and Stokes viscous resistance of magnetic beads were analyzed and simulated quantificationally; finally the magnetic bead capture area was got under different flow velocity. Consequently the feasibility and correctness of this method was verified.

scholarly journals A single-cell RNA-sequencing training and analysis suite using the Galaxy framework

GigaScience ◽  
2020 ◽  
Vol 9 (10) ◽  
Author(s):  
Mehmet Tekman ◽  
Bérénice Batut ◽  
Alexander Ostrovsky ◽  
Christophe Antoniewski ◽  
Dave Clements ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
Cell Biology ◽  
High Performance ◽  
Learning Experience ◽  
Sequencing Analysis ◽  
Teaching Resources ◽  
Single Cell Rna Sequencing ◽  
The Galaxy ◽  
Downstream Analysis

Abstract Background The vast ecosystem of single-cell RNA-sequencing tools has until recently been plagued by an excess of diverging analysis strategies, inconsistent file formats, and compatibility issues between different software suites. The uptake of 10x Genomics datasets has begun to calm this diversity, and the bioinformatics community leans once more towards the large computing requirements and the statistically driven methods needed to process and understand these ever-growing datasets. Results Here we outline several Galaxy workflows and learning resources for single-cell RNA-sequencing, with the aim of providing a comprehensive analysis environment paired with a thorough user learning experience that bridges the knowledge gap between the computational methods and the underlying cell biology. The Galaxy reproducible bioinformatics framework provides tools, workflows, and trainings that not only enable users to perform 1-click 10x preprocessing but also empower them to demultiplex raw sequencing from custom tagged and full-length sequencing protocols. The downstream analysis supports a range of high-quality interoperable suites separated into common stages of analysis: inspection, filtering, normalization, confounder removal, and clustering. The teaching resources cover concepts from computer science to cell biology. Access to all resources is provided at the singlecell.usegalaxy.eu portal. Conclusions The reproducible and training-oriented Galaxy framework provides a sustainable high-performance computing environment for users to run flexible analyses on both 10x and alternative platforms. The tutorials from the Galaxy Training Network along with the frequent training workshops hosted by the Galaxy community provide a means for users to learn, publish, and teach single-cell RNA-sequencing analysis.

scholarly journals Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 308 ◽  
Author(s):  
Phalguni Tewari Kumar ◽  
Deborah Decrop ◽  
Saba Safdar ◽  
Ioannis Passaris ◽  
Tadej Kokalj ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Cell Sorting ◽  
Magnetic Beads ◽  
Single Cells ◽  
Digital Microfluidics ◽  
Cell Behavior ◽  
Bacterial Cells ◽  
Microfluidic Platforms ◽  
Dynamic Cell ◽  
Downstream Analysis

When screening microbial populations or consortia for interesting cells, their selective retrieval for further study can be of great interest. To this end, traditional fluorescence activated cell sorting (FACS) and optical tweezers (OT) enabled methods have typically been used. However, the former, although allowing cell sorting, fails to track dynamic cell behavior, while the latter has been limited to complex channel-based microfluidic platforms. In this study, digital microfluidics (DMF) was integrated with OT for selective trapping, relocation, and further proliferation of single bacterial cells, while offering continuous imaging of cells to evaluate dynamic cell behavior. To enable this, magnetic beads coated with Salmonella Typhimurium-targeting antibodies were seeded in the microwell array of the DMF platform, and used to capture single cells of a fluorescent S. Typhimurium population. Next, OT were used to select a bead with a bacterium of interest, based on its fluorescent expression, and to relocate this bead to a different microwell on the same or different array. Using an agar patch affixed on top, the relocated bacterium was subsequently allowed to proliferate. Our OT-integrated DMF platform thus successfully enabled selective trapping, retrieval, relocation, and proliferation of bacteria of interest at single-cell level, thereby enabling their downstream analysis.

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