S-nitrosylation of GAD65 is implicated in decreased GAD activity and oxygen-induced seizures

2017 ◽  
Vol 653 ◽  
pp. 283-287 ◽  
Author(s):  
Heath G. Gasier ◽  
Ivan T. Demchenko ◽  
Lynn G. Tatro ◽  
Claude A. Piantadosi
Keyword(s):  
Gad Activity

scholarly journals Decrease of glutamate decarboxylase (GAD)-immunoreactive nerve terminals in the substantia nigra after kainic acid lesion of the striatum.

1981 ◽  
Vol 29 (8) ◽  
pp. 977-980 ◽  
Author(s):  
W H Oertel ◽  
D E Schmechel ◽  
M J Brownstein ◽  
M L Tappaz ◽  
D H Ransom ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Substantia Nigra ◽  
Kainic Acid ◽  
Glutamate Decarboxylase ◽  
Marked Reduction ◽  
Immunohistochemical Localization ◽  
Nerve Terminals ◽  
Immunohistochemical Examination ◽  
Axon Terminals ◽  
Gad Activity

Antiserum sheep 3 against rat brain glutamate decarboxylase (GAD) was employed for the immunohistochemical localization of GAD-immunoreactive nerve terminals in the substantia nigra (SN). To test whether the antiserum specifically localized GAD-containing axon terminals, the effect of kainic acid-induced striatal lesions on the reactive nerve endings in the SN was investigated. Seven days after the injection of 1 microgram kainic acid into the striatum, a 65% decrease in GAD-enzyme activity occurred in the ipsilateral SN. On immunohistochemical examination there was correspondingly a marked reduction of GAD-positive terminals. The parallel decrease in biochemical and immunohistochemical GAD activity indicates that antiserum sheep 3 can be used as a specific immunohistochemical probed for GAD-containing elements.

Cerebral GABA release and GAD activity in protein- and tryptophan-restricted rats during development

2002 ◽  
Vol 20 (1) ◽  
pp. 47-54 ◽  
Author(s):  
A.R. Del Angel-Meza ◽  
L. Ramı́rez-Cortes ◽  
I.G. Adame-González ◽  
I. González Burgos ◽  
C. Beas-Zárate
Keyword(s):  
Gaba Release ◽  
Gad Activity

scholarly journals Glutamate Decarboxylase-Mediated Nisin Resistance in Listeria monocytogenes

2010 ◽  
Vol 76 (19) ◽  
pp. 6541-6546 ◽  
Author(s):  
Máire Begley ◽  
Paul D. Cotter ◽  
Colin Hill ◽  
R. Paul Ross
Keyword(s):  
Listeria Monocytogenes ◽  
Glutamate Decarboxylase ◽  
Low Ph ◽  
Specific Inhibition ◽  
General Phenomenon ◽  
Growth And Survival ◽  
Gram Positive Bacteria ◽  
Complete Set ◽  
Gad Activity ◽  
Is Strain

ABSTRACT Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain variable and is found only in approximately 50% of L. monocytogenes strains. Growth and survival experiments revealed that possession of gadD1 correlates with a higher degree of tolerance to nisin. Significantly, a similar finding using a gadB mutant of L. lactis IL1403 implies that this may be a general phenomenon in Gram-positive bacteria. Our findings thus suggest that the specific inhibition of GAD activity or a reduction in the levels of free glutamate may prevent the growth of otherwise resistant GAD+ bacteria in foods where low pH and/or nisin is used as a preservative.

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