scholarly journals Sex Differences in Neuroplasticity- and Stress-Related Gene Expression and Protein Levels in the Rat Hippocampus Following Oxycodone Conditioned Place Preference

Neuroscience ◽  
2019 ◽  
Vol 410 ◽  
pp. 274-292 ◽  
Author(s):  
Matthew Randesi ◽  
Natalina H. Contoreggi ◽  
Yan Zhou ◽  
Batsheva R. Rubin ◽  
Julia R. Bellamy ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sex Differences ◽  
Conditioned Place Preference ◽  
Place Preference ◽  
Rat Hippocampus ◽  
Related Gene ◽  
Protein Levels

scholarly journals Vulnerability factors for mephedrone-induced conditioned place preference in rats—the impact of sex differences, social-conditioning and stress

2021 ◽  
Author(s):  
Olga Wronikowska ◽  
Maria Zykubek ◽  
Łukasz Kurach ◽  
Agnieszka Michalak ◽  
Anna Boguszewska-Czubara ◽  
...  
Keyword(s):  
Sex Differences ◽  
Conditioned Place Preference ◽  
Low Dose ◽  
Social Factor ◽  
Place Preference ◽  
Female Rats ◽  
Male Rats ◽  
Social Conditioning ◽  
Male And Female Rats ◽  
The Impact

Abstract Rationale Mephedrone is a frequently overused drug of abuse that belongs to the group of novel psychoactive substances. Although its mechanism of action, as well as toxic and psychoactive effects, has been widely studied, the role of different factors that could contribute to the increased vulnerability to mephedrone abuse is still poorly understood. Objectives The aim of the presented study was to assess the impact of several factors (sex differences, social-conditioning, and chronic mild unpredictable stress — CMUS) on the liability to mephedrone-induced reward in Wistar rats. Methods The rewarding effects of mephedrone in male and female rats were assessed using the conditioned place preference (CPP) procedure. Furthermore, the impact of social factor and stress was evaluated in male rats using social-CPP and CMUS-dependent CPP, respectively. Results Mephedrone induced classic-CPP in female (10 mg/kg), as well as in male (10 and 20 mg/kg) rats. However, the impact of mephedrone treatment during social-CPP was highly dose-dependent as the rewarding effects of low dose of mephedrone (5 mg/kg; non-active in classic-CPP) were potentiated when administered during social-conditioning. Interestingly, social-conditioning with a higher dose of 20 mg/kg (that induced classic-CPP) was able to reverse these effects. Finally, CMUS potentiated rewarding effects of a low dose of mephedrone (5 mg/kg) and increased the level of corticosterone in rats’ prefrontal cortex and hippocampus. Conclusions Altogether, the presented results give new insight into possible factors underlying the vulnerability to mephedrone abuse and can serve as a basis for further studies assessing mechanisms underlying observed effects.

scholarly journals Sex differences in pregabalin-seeking like behavior in a conditioned place preference paradigm

2020 ◽  
Vol 28 (12) ◽  
pp. 1749-1755
Author(s):  
Hashem O. Alsaab ◽  
Ebtehal Altowairqi ◽  
Nada Alzahrani ◽  
Reem Alzahrani ◽  
Fahad S. Alshehri ◽  
...  
Keyword(s):  
Sex Differences ◽  
Conditioned Place Preference ◽  
Place Preference ◽  
Conditioned Place Preference Paradigm

Sex differences in luteinizing hormone releasing hormone-induced conditioned place preference in the rat

1989 ◽  
Vol 16 (2-4) ◽  
pp. 375-383 ◽  
Author(s):  
Rene de Beun ◽  
Nora E. Geerts ◽  
Nanne E. van de Poll ◽  
Jef L. Slangen ◽  
Jan T. M. Vreeburg
Keyword(s):  
Sex Differences ◽  
Luteinizing Hormone ◽  
Conditioned Place Preference ◽  
Place Preference ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone

A Maternal Low-protein Diet during Gestation Induces Hepatic Autophagy-related Gene Expression in a Sex-specific Manner in Sprague-Dawley Rats

2021 ◽  
pp. 1-29
Author(s):  
Mingzhu Cai ◽  
Jie Zhang ◽  
Hong Chen ◽  
Yuan-Xiang Pan
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Control Diet ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Related Gene ◽  
Sprague Dawley ◽  
Protein Levels ◽  
Low Protein ◽  
Sprague Dawley Rats

Abstract This study investigates the mechanism by which maternal protein restriction induces hepatic autophagy-related gene expression in the offspring of rats. Pregnant Sprague-Dawley rats were fed either a control diet (C, 18% energy from protein) or a low-protein diet (LP, 8.5% energy from protein) during gestation, followed by the control diet during lactation and post-weaning. Liver tissue was collected from the offspring at postnatal day 38 and divided into four groups according to sex and maternal diet (F-C, F-LP, M-C, and M-LP) for further analysis. Autophagy-related mRNA and protein levels were determined by real-time PCR and Western blotting, respectively. In addition, chromatin immunoprecipitation (ChIP) was performed to investigate the interactions between transcription factors and autophagy-related genes. Protein levels of p-eIF2a and ATF4 were increased only in the female offspring born to dams fed the LP diet. Correlatively, the mRNA expression of hepatic autophagy-related genes including Map1lc3b, P62/Sqstm1, Becn1, Atg3, Atg7, and Atg10 was significantly greater in the F-LP group than in the F-C group. Furthermore, ChIP results showed greater ATF4 and C/EBP homology protein (CHOP) binding at the regions of a set of autophagy-related genes in the F-LP group than in the F-C group. Our data demonstrated that a maternal LP diet transcriptionally programmed hepatic autophagy-related gene expression only in female rat offspring. This transcriptional program involved the activation of the eIF2α/ATF4 pathway and intricate regulation by transcription factors ATF4 and CHOP.

scholarly journals Sex Differences in the Rat Hippocampal Opioid System After Oxycodone Conditioned Place Preference

Neuroscience ◽  
2018 ◽  
Vol 393 ◽  
pp. 236-257 ◽  
Author(s):  
James D. Ryan ◽  
Yan Zhou ◽  
Natalina H. Contoreggi ◽  
Farah K. Bshesh ◽  
Jason D. Gray ◽  
...  
Keyword(s):  
Sex Differences ◽  
Conditioned Place Preference ◽  
Place Preference ◽  
Opioid System

Sex differences in dopamine binding and modafinil conditioned place preference in mice

2015 ◽  
Vol 155 ◽  
pp. 37-44 ◽  
Author(s):  
Rick E. Bernardi ◽  
Laura Broccoli ◽  
Rainer Spanagel ◽  
Anita C. Hansson
Keyword(s):  
Sex Differences ◽  
Conditioned Place Preference ◽  
Place Preference

Conditioned Place Preference Associated with Level of Palmitoylation of PSD-95 in Rat Hippocampus and Nucleus Accumbens

2011 ◽  
Vol 64 (4) ◽  
pp. 211-218 ◽  
Author(s):  
Xiaojie Zhang ◽  
Xuebing Liu ◽  
Daijun Wang ◽  
Haidan Liu ◽  
Wei Hao
Keyword(s):  
Nucleus Accumbens ◽  
Conditioned Place Preference ◽  
Place Preference ◽  
Rat Hippocampus

Cannabidiol prevents priming- and stress-induced reinstatement of the conditioned place preference induced by cocaine in mice

2021 ◽  
pp. 026988112096595
Author(s):  
Claudia Calpe-López ◽  
Ani Gasparyan ◽  
Francisco Navarrete ◽  
Jorge Manzanares ◽  
Jose Miñarro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ventral Tegmental Area ◽  
Conditioned Place Preference ◽  
Cannabis Sativa ◽  
Social Defeat ◽  
Place Preference ◽  
Cocaine Seeking ◽  
Ventral Tegmental ◽  
Relative Gene ◽  
Reinstatement Test

Background: Cocaine dependence is an important problem without any effective pharmacological treatment. Some preclinical studies have suggested that cannabidiol (CBD), a component of the Cannabis sativa plant, could be useful for the treatment of cocaine use disorders. Aims: This work aims to evaluate the ability of CBD to reduce priming- and stress-induced reinstatement of the conditioned place preference (CPP) induced by cocaine. Methods: Young adult CD-1 male mice were allocated to 10 groups ( n = 12/group), conditioned with cocaine (10 mg/kg) and exposed to extinction of CPP (two sessions per week). When extinction was achieved, each group received the corresponding treatment before the reinstatement test. In experiment 1, six groups were used: vehicle+saline (Veh+Sal), 5 mg/kg cocaine alone (Veh+Coc) or with CBD 30 or 60 mg/kg (CBD30+Coc, CBD60+Coc) and CBD alone (CBD30+Sal, CBD60+Sal). In experiment 2, four groups were used: exploration (Veh+Expl), social defeat (Veh+SD) and social defeat with CBD (CBD30+SD and CBD60+SD). Furthermore, the relative gene expression of the dopamine transporter (DAT) in the ventral tegmental area was measured. Results: All mice acquired cocaine CPP and extinguished it after three or four weeks. Only the groups treated with cocaine priming (Veh+Coc) or exposed to social defeat (Veh+SD) showed reinstatement of CPP. Interestingly, CBD itself did not induce reinstatement and blocked the reinstating effects of cocaine priming and social defeat. Furthermore, cocaine priming increased DAT gene expression in the ventral tegmental area and CBD completely reversed this effect. Conclusion: These results suggest that CBD could reduce reinstatement to cocaine seeking after a period of abstinence.

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