Nuclear surface parameters revisited

2021 ◽  
pp. 122376
Author(s):  
A.R. Abdulghany ◽  
A.H. Hanafy
Author(s):  
Awtar Krishan ◽  
Nestor Bohonos

Cytochalasin B, a mould metabolite from Helminthosporium dermatioideum has been shown to interfere with specific cell activities such as cytoplasmic cleavage and cell movement. Cells undergoing nuclear division in the presence of cytochalasin B are unable to complete the separation of the resulting daughter cells. In time-lapse studies, the daughter cells coalesce after an initial unsuccessful attempt at separation and form large multinucleate polyploid cells. The present report describes the fine structure of the large polyploid cells induced in Earle's L-cell monolayer cultures by exposure to cytochalasin B (lγ/ml) for 92 hours.In the present material we have seen as many as 7 nuclei in these polyploid cells. Treatment with cytochalasin B for longer periods of time (6 to 7 days, with one medium change on the 3rd day) did not increase the number of nuclei beyond the 7 nuclei stage. Figure 1 shows a large polyploid cell with four nuclei. These nuclei are indistinguishable in their fine structure from those of the cells from control cultures but often show unusually large numbers of cytoplasmic invaginations and extensions of the nuclear surface (Figure 2).


Author(s):  
W.F. Marshall ◽  
A.F. Dernburg ◽  
B. Harmon ◽  
J.W. Sedat

Interactions between chromatin and nuclear envelope (NE) have been implicated in chromatin condensation, gene regulation, nuclear reassembly, and organization of chromosomes within the nucleus. To further investigate the physiological role played by such interactions, it will be necessary to determine which loci specifically interact with the nuclear envelope. This will not only facilitate identification of the molecular determinants of this interaction, but will also allow manipulation of the pattern of chromatin-NE interactions to probe possible functions. We have developed a microscopic approach to detect and map chromatin-NE interactions inside intact cells.Fluorescence in situ hybridization (FISH) is used to localize specific chromosomal regions within the nucleus of Drosophila embryos and anti-lamin immunofluorescence is used to detect the nuclear envelope. Widefield deconvolution microscopy is then used to obtain a three-dimensional image of the sample (Fig. 1). The nuclear surface is represented by a surface-harmonic expansion (Fig 2). A statistical test for association of the FISH spot with the surface is then performed.


Water ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 388
Author(s):  
Hao Cheng ◽  
Liang Sun ◽  
Jiagen Li

The extraction of physical information about the subsurface ocean from surface information obtained from satellite measurements is both important and challenging. We introduce a back-propagation neural network (BPNN) method to determine the subsurface temperature of the North Pacific Ocean by selecting the optimum input combination of sea surface parameters obtained from satellite measurements. In addition to sea surface height (SSH), sea surface temperature (SST), sea surface salinity (SSS) and sea surface wind (SSW), we also included the sea surface velocity (SSV) as a new component in our study. This allowed us to partially resolve the non-linear subsurface dynamics associated with advection, which improved the estimated results, especially in regions with strong currents. The accuracy of the estimated results was verified with reprocessed observational datasets. Our results show that the BPNN model can accurately estimate the subsurface (upper 1000 m) temperature of the North Pacific Ocean. The corresponding mean square errors were 0.868 and 0.802 using four (SSH, SST, SSS and SSW) and five (SSH, SST, SSS, SSW and SSV) input parameters and the average coefficients of determination were 0.952 and 0.967, respectively. The input of the SSV in addition to the SSH, SST, SSS and SSW therefore has a positive impact on the BPNN model and helps to improve the accuracy of the estimation. This study provides important technical support for retrieving thermal information about the ocean interior from surface satellite remote sensing observations, which will help to expand the scope of satellite measurements of the ocean.


2021 ◽  
Vol 87 (2) ◽  
Author(s):  
Arthur Carlton-Jones ◽  
Elizabeth J. Paul ◽  
William Dorland

Coil complexity is a critical consideration in stellarator design. The traditional two-step optimization approach, in which the plasma boundary is optimized for physics properties and the coils are subsequently optimized to be consistent with this boundary, can result in plasma shapes which cannot be produced with sufficiently simple coils. To address this challenge, we propose a method to incorporate considerations of coil complexity in the optimization of the plasma boundary. Coil complexity metrics are computed from the current potential solution obtained with the REGCOIL code (Landreman, Nucl. Fusion, vol. 57, 2017, 046003). While such metrics have previously been included in derivative-free fixed-boundary optimization (Drevlak et al., Nucl. Fusion, vol. 59, 2018, 016010), we compute the local sensitivity of these metrics with respect to perturbations of the plasma boundary using the shape gradient (Landreman & Paul, Nucl. Fusion, vol. 58, 2018, 076023). We extend REGCOIL to compute derivatives of these metrics with respect to parameters describing the plasma boundary. In keeping with previous research on winding surface optimization (Paul et al., Nucl. Fusion, vol. 58, 2018, 076015), the shape derivatives are computed with a discrete adjoint method. In contrast with the previous work, derivatives are computed with respect to the plasma surface parameters rather than the winding surface parameters. To further reduce the resolution required to compute the shape gradient, we present a more efficient representation of the plasma surface which uses a single Fourier series to describe the radial distance from a coordinate axis and a spectrally condensed poloidal angle. This representation is advantageous over the standard cylindrical representation used in the VMEC code (Hirshman & Whitson, Phys. Fluids, vol. 26, 1983, pp. 3553–3568), as it provides a uniquely defined poloidal angle, eliminating a null space in the optimization of the plasma surface. In comparison with previous spectral condensation methods (Hirshman & Breslau, Phys. Plasmas, vol. 5, 1998, p. 2664), the modified poloidal angle is obtained algebraically rather than through the solution of a nonlinear optimization problem. The resulting shape gradient highlights features of the plasma boundary that are consistent with simple coils and can be used to couple coil and fixed-boundary optimization.


1972 ◽  
Vol 45 ◽  
pp. 265-270
Author(s):  
L. M. Shul'man

The probable parent-molecules of radicals such as C3 and N2+ are discussed, and it is concluded that cometary nuclei may contain complicated organic molecules, such as C3H4, CH2N2, and C4H2. It is suggested that these molecules are formed by radiation synthesis in solid phase. In a time interval of order 107 to 109 yr bombardment from cosmic rays would be expected to transform the chemical composition to a depth of 1 m. Solar cosmic rays do not penetrate as far, and as a result the surface layer of the nucleus can be enriched with unsaturated hydrocarbons. After a critical concentration of this explosive material is reached a further burst of solar cosmic rays can initiate an explosion and thus an outburst in the comet's brightness. This mechanism is the only one advanced to date that can explain the synchronism of the energy output over the whole nuclear surface.


1980 ◽  
Vol 57 (2) ◽  
pp. 205-214 ◽  
Author(s):  
A. R. Farhan ◽  
H. Überall ◽  
O. Dragún ◽  
E. Maqueda

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