Molecular identification of the causative agent of human strongyloidiasis acquired in Tanzania: Dispersal and diversity of Strongyloides spp. and their hosts

2010 ◽  
Vol 59 (3) ◽  
pp. 407-413 ◽  
Author(s):  
Hideo Hasegawa ◽  
Hiroshi Sato ◽  
Shiho Fujita ◽  
Pierre Philippe Mbehang Nguema ◽  
Kenichi Nobusue ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Causative Agent

scholarly journals Ocular Dirofilariasis Case in Thailand Confirmed by Molecular Analysis to Be Caused by Dirofilaria immitis

2021 ◽  
Author(s):  
Yuwadee Somsap ◽  
Patcharaporn Boonroumkaew ◽  
Attawit Somsap ◽  
Rutchanee Rodpai ◽  
Lakkhana Sadaow ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Male Patient ◽  
Molecular Identification ◽  
Causative Agent ◽  
Clinical Characteristics ◽  
Dirofilaria Immitis ◽  
Anterior Part ◽  
Zoonotic Disease ◽  
Tissue Growth ◽  
Cuticular Surface

A rare ocular dirofilariasis case along with the clinical characteristics, treatment, and outcome is reported. A whitish roundworm (10.6 cm long and 0.5 mm width) emerged from the pterygium, a triangular tissue growth on the cornea of the eye, of a male patient. The worm had a rounded anterior part, mouth without lips, smooth cuticular surface, and short rounded posterior tail with spicules: these features suggested that it was a male Dirofilaria sp. Molecular identification confirmed that the worm belonged to Dirofilaria immitis. This is the first molecular confirmation that D. immitis is a causative agent of ocular dirofilariasis in Thailand: dirofilariasis is a newly emerging zoonotic disease. Physicians should be alert to zoonotic filarial worms and knowledgeable about treatment of this disease.

scholarly journals Avian Mycobacteriosis and Molecular Identification of Mycobacterium avium Subsp. avium in Racing Pigeons (Columba livia domestica) in Greece

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 291
Author(s):  
Vasilios Tsiouris ◽  
Konstantinos Kiskinis ◽  
Tilemachos Mantzios ◽  
Chrysostomos Dovas ◽  
Natalia Mavromati ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Mycobacterium Avium ◽  
Causative Agent ◽  
Clinical Signs ◽  
Columba Livia ◽  
Mycobacterium Avium Subsp ◽  
Tissue Sections ◽  
First Case ◽  
Femoral Bone Marrow ◽  
Periorbital Swelling

In this report, cases of avian mycobacteriosis in two lofts of racing pigeons are described. Three racing pigeons of 2-year old from the first loft (A) and four racing pigeons of 4–5 years old from the second loft (B) were submitted to the Unit of Avian Medicine for clinical examination and necropsy. In the case history chronic and debilitating disease was reported. The clinical signs included emaciation, depression, lameness, periorbital swelling and diarrhea, although the appetite was normal. Post mortem lesions involved an enlarged spleen with multiple different sized yellow nodules. Similar lesions were also observed in the liver, conjunctiva of the inferior eyelids and in the femoral bone marrow. The suspicion of avian mycobacteriosis was based on history, clinical signs and typical lesions. In order to confirm the diagnosis, histopathology was performed on tissue sections and revealed the presence of multiple granulomas with central necrosis. In addition, Ziehl-Neelsen positive bacilli were observed in histological sections and smears from the granulomas of the affected tissues. Molecular analysis identified the causative agent as Mycobacterium avium subsp. avium. This is the first case report of avian mycobacteriosis in Greece, which describes the presence of granulomatous conjunctivitis and the molecular identification of M. avium subsp. avium as the causative agent in racing pigeons.

scholarly journals Molecular Identification of Adenocephalus pacificus (Cestoda) from Three Human Cases in Lima Province, Peru

2020 ◽  
Vol 58 (4) ◽  
pp. 457-460
Author(s):  
Aarón Mondragón-Martínez ◽  
Rosa Martínez-Rojas ◽  
Enrique Garcia-Candela ◽  
Abraham Delgado-Escalante ◽  
Manuel Tantaleán-Vidaurre ◽  
...  
Keyword(s):  
Cytochrome C ◽  
South America ◽  
Cytochrome C Oxidase ◽  
Marine Fish ◽  
Molecular Identification ◽  
Causative Agent ◽  
Pacific Coast ◽  
The Other ◽  
Partial Sequence ◽  
The Pacific

The Pacific tapeworm Adenocephalus pacifcus (syn. Diphyllobothrium pacificum) is a causative agent of diphyllobothriosis occurred in Pacific coast of South America, mainly in Peru. Source of infections are traditional meal from raw or undercooked marine fish such as “cebiche”. We confirmed 3 new cases, one including scolex and the other two headless. A strobila 46 cm long without scolex was discharged from an 8-year-old boy before treatment. Specimens were confirmed morphologically by presence of tegumental protuberances on proglottids and small sized eggs. Partial sequence of cytochrome c oxidase subunit 1 gene was congruent with A. pacificus sequences.

scholarly journals Molecular identification of Vibrio tapetis, the causative agent of the brown ring disease of Ruditapes philippinarum

Aquaculture ◽  
2006 ◽  
Vol 253 (1-4) ◽  
pp. 25-38 ◽  
Author(s):  
Christine Paillard ◽  
Sabrina Gausson ◽  
Jean Louis Nicolas ◽  
Jean Paul le Pennec ◽  
Dominique Haras
Keyword(s):  
Molecular Identification ◽  
Causative Agent ◽  
Ruditapes Philippinarum ◽  
Brown Ring Disease

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

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