Differences in femoral blood sampling techniques on total post mortem tryptase

Pathology ◽  
2018 ◽  
Vol 50 ◽  
pp. S97
Author(s):  
R. Tse ◽  
J. Garland ◽  
K. Kesha ◽  
H. Elstub ◽  
A.D. Cala ◽  
...  
Keyword(s):  
Blood Sampling ◽  
Sampling Techniques ◽  
Post Mortem ◽  
Femoral Blood

Interpretation of melperone intoxication: post-mortem concentration distribution and interpretation of intoxication data

2021 ◽  
Vol 36 (3) ◽  
pp. 233-237
Author(s):  
Marek Dziadosz ◽  
Katarina Bolte ◽  
Wolfgang Rosenberger ◽  
Michael Klintschar ◽  
Jörg Teske
Keyword(s):  
Concentration Distribution ◽  
Method Development ◽  
Standard Addition ◽  
Lethal Concentration ◽  
Post Mortem ◽  
Tissue Samples ◽  
Expert Opinions ◽  
Lack Of Information ◽  
Reference Concentration ◽  
Femoral Blood

Abstract Objectives Since melperone abuse with lethal intoxication is common, expert opinions based on therapeutical and lethal concentration ranges can be considered as important. Because there is a lack of information about fatalities caused by melperone mono-intoxications and data on tissue samples with concentration distribution, the aim of this work is the examination of lethal concentration ranges of melperone and drug quantification in different matrices. Methods An LC-MS/MS method was applied for analyses performed in blood and tissue samples. Quantification based on standard addition and sample preparation on liquid–liquid extraction with 1-chlorobutane. An appropriate tissue homogenization was performed ahead of extraction with an IKA Ultra-Turrax-Tube-Drive®. A Luna 5 µm C18 (2) 100 Å, 150  × 2 mm analytical column was used for chromatographic separation and the elution was performed with two mobile phases consisted of A (H2O/methanol = 95/5, v/v) and B (H2O/methanol = 3/97, v/v) both with 10 mM ammonium acetate and 0.1% acetic acid. Results A multi-drug LC-MS/MS analytical method developed was applied successfully for melperone quantification in different post-mortem matrices. No analytical problems could be identified during method development and analyses of real samples. The melperone lethal concentration calculated in femoral blood of the drug mono-intoxication investigated was 10 mg/L. Melperone concentration distribution was presented for the first time. Conclusions The lethal reference concentration of melperone in femoral blood of 17.1 mg/L pointed out in different reference lists should be used with caution. Instead, a lower lethal melperone concentration should be considered. The post-mortem concentration distribution of the drug presented could be helpful in the interpretation of cases where no blood samples are available.

Femoral blood concentrations of the designer benzodiazepine etizolam in post-mortem cases

2020 ◽  
pp. 002580242097381
Author(s):  
Laura J Hikin ◽  
Paul R Smith ◽  
Peter D Maskell ◽  
Hassan Kurimbokus ◽  
Emily Ashong ◽  
...  
Keyword(s):  
Drug Abusers ◽  
Post Mortem ◽  
Limited Data ◽  
Spectrometry Method ◽  
Blood Concentrations ◽  
Blunt Force Trauma ◽  
The World ◽  
Femoral Blood ◽  
The Mean ◽  
Multiple Drugs

Etizolam is a thienodiazepine that although licensed for clinical usage in Japan, India and South Korea is commonly abused and detected in post-mortem cases around the world. To date, there are limited data in the literature to allow for the interpretation of blood concentrations of etizolam in post-mortem cases. A liquid chromatography with tandem mass spectrometry method was used to quantitate etizolam concentrations in 28 post-mortem cases where etizolam was detected. The median concentration of etizolam in femoral blood was 8.5 ng/mL (range 1.0–172.0 ng/mL; n = 24); in antemortem plasma, the etizolam concentration range was 4–44 ng/mL ( n = 4). The mean age of the individuals abusing etizolam was 38.5 ± 8.4 years (median 39 years), with the majority being male (86%). In all of the cases, multiple drugs were detected, with the most common being pregabalin (61%) followed by morphine/heroin (54%), diazepam (54%) and benzoylecgonine (21%), illustrating the increasing problem of poly-substance use in drug abusers. The cause of death in the cases in which etizolam was detected was multi-drug toxicity in 87.5% of the cases, with 12.5% unrelated to drug use (hangings and blunt-force trauma). These data will further help forensic practitioners with the interpretation of post-mortem etizolam concentrations.

Blood sampling techniques for studying rapidly turning over metabolic fuels in mice

Lipids ◽  
1976 ◽  
Vol 11 (11) ◽  
pp. 818-820 ◽  
Author(s):  
Nome Baker ◽  
Daniel Morris ◽  
Christy Sandborg
Keyword(s):  
Blood Sampling ◽  
Sampling Techniques

scholarly journals Impact of three commonly used blood sampling techniques on the welfare of laboratory mice: Taking the animal’s perspective

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0238895 ◽  
Author(s):  
Neele Meyer ◽  
Mareike Kröger ◽  
Julia Thümmler ◽  
Lisa Tietze ◽  
Rupert Palme ◽  
...  
Keyword(s):  
Blood Sampling ◽  
Sampling Techniques ◽  
Laboratory Mice

scholarly journals Predictive equation to estimate post-mortem interval using spectrophotometric blood-colour values

2019 ◽  
Vol 59 (1) ◽  
pp. 36-41
Author(s):  
Yosuke Usumoto ◽  
Keiko Kudo ◽  
Akiko Tsuji ◽  
Yoko Ihama ◽  
Noriaki Ikeda
Keyword(s):  
Right Ventricular ◽  
Left Ventricular ◽  
Predictive Equation ◽  
Mean Square ◽  
Post Mortem ◽  
Predictive Equations ◽  
Post Mortem Interval ◽  
Lower Root ◽  
Femoral Blood ◽  
Left And Right

Forensic pathologists use post-mortem phenomena to estimate the post-mortem interval (PMI). We have reported on the usefulness of post-mortem lividity spectrophotometric values to estimate PMIs. Here, we focused on blood colour, looking for associations between blood colour, age and PMI. We generated predictive equations for blood-colour values and the PMI. We included data from a total of 129 cadavers (84 males and 45 females). We measured the colour of 124 left ventricular blood ( L*l, a*l, b*l), 123 right ventricular blood ( L*r, a*r, b*r) and 57 femoral blood ( L*f, a*f, b*f) samples. We found no significant associations between blood colour and age or between blood colour and the PMI, but the values of a*l, b*l, a*r and b*r were significantly increased with increased age, and those of L*f, a*f and b*f were significantly decreased with increased PMI. We created equations to estimate blood colour. The equations for femoral blood colour had higher adjusted R2 values and lower root mean square error values than those for left and right ventricular blood colours. We generated equations to estimate PMIs using blood-colour values and autopsy findings. Our estimated PMIs up to 67 hours had accuracies within 8.84 hours, without measuring post-mortem lividity colour or considering the age of the deceased. This is the first study to estimate PMIs based on blood-colour spectrophotometric values.

Patterns of LH and FSH in men during high-frequency blood sampling

1987 ◽  
Vol 114 (1) ◽  
pp. 153-160 ◽  
Author(s):  
F. Scheele ◽  
C. B. Lambalk ◽  
J. Schoemaker ◽  
H. van Kessel ◽  
J. de Koning ◽  
...  
Keyword(s):  
High Frequency ◽  
Small Amplitude ◽  
Sampling Technique ◽  
Blood Sampling ◽  
Sampling Techniques ◽  
Hormonal Changes ◽  
Rubber Stopper ◽  
Heparinized Saline ◽  
Physiological Phenomenon ◽  
The Difference

ABSTRACT The aim of the study was to test the hypothesis that in serial determinations of concentrations of LH and FSH involving blood samples taken every minute, the observed pulses of LH and FSH which last less than 3–4 min might not be a physiological phenomenon but part of the 'noise' of the radioimmunoassay or blood-sampling technique. Blood was sampled every minute for a period of 90 min in six men. During the first 45 min, blood was sampled by means of vacuum tubes only. During the second 45 min, sampling took place with a syringe via a rubber stopper, either using a tourniquet (n = 3) or flushing the cannula with heparinized saline. Three criteria were used to identify variations in the patterns of LH and FSH as true hormonal changes. First, a threshold was used which had to be exceeded by the difference between nadir and maximum values before a pulse could be identified. An average of approximately six pulses per 90 min was found in both the LH and FSH series. The majority of these pulses lasted less than 3–4 min. In two subjects, larger LH pulses of longer duration were measured. Secondly, differences between duplicate measurements of nadir and/or maximum values of more than one-third of the amplitude of a pulse were considered unacceptable. This involved about 75% of the pulses. Thirdly, the reproducibility of the hormone variations was estimated. In one subject, concentrations of LH were measured four times in four separate assays. Measurement of FSH concentrations in this subject and of LH and FSH in the samples from the other five subjects were repeated once again, but only in those parts of the series of samples which had shown hormone variations beyond the threshold composed of acceptable duplicate measurements. Only the larger variations of longer duration, as found earlier in two of the LH series, were reproducible. The different blood-sampling techniques used had no significant influence on the frequency of pulses. It was concluded that a rapid, small amplitude, pulsatile pattern of release of LH and FSH is probably not present in men or is obscured by limitations of current techniques of radioimmunoassay. J. Endocr. (1987) 114, 153–160

scholarly journals Blood Glucose Analysis with Different Blood Sampling Techniques in Chick Embryos

2021 ◽  
Vol Volum: 3, Issue: 3 (Volum: 3, Issue: 3) ◽  
pp. 224-228
Author(s):  
Emre Atay ◽  
Erhan Bozkurt ◽  
Abdülkadir Bilir ◽  
Ayşe Ertekin ◽  
Ayhan Vurmaz
Keyword(s):  
Blood Glucose ◽  
Blood Sampling ◽  
Sampling Techniques ◽  
Chick Embryos ◽  
Glucose Analysis
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