Bianca Andrade Handan
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Carolina Foot Gomes De Moura
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Caroline Margonato Cardoso
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Aline Boveto Santamarina
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Luciana Pellegrini Pisani
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...
Abstract
Background The objective of this study was to evaluate protective effect
of grape and apple juices against toxicity induced by cadmium in the kidney of
rats.
Methods A total of 20 male-Wistar rats were distributed into four groups
(n=5): Control group: animals received an intraperitoneal (i.p.)
injection of 0.9% saline solution and after 15 days, 1 mL of
water was administered for 15 days, via gavage; Cadmium group: animals received
an intraperitoneal injection of cadmium chloride (1.2 mg/kg) and
after 15 days, 1 mL of water was administered for 15 days via gavage;
Cadmium+Grape Juice: animals received an i.p. injection of cadmium
chloride (1.2 mg/kg), and after 15 days, 0.8 mL of grape
juice was administered for 15 days, via gavage; Cadmium+Apple Juice:
animals received i.p. injection of cadmium chloride (1.2 mg/kg)
and after 15 days, 1.0 mL of apple juice was administered for 15 days,
via gavage.
Results Histopathological analysis revealed severe tubular lesion and
necrosis in the group exposed to cadmium, while animals exposed to grape or
apple juices showed a significant reduction of tissue injury. 8-OHdG
immunoexpression, DNA damage, cytochrome C and catalase gene expressions and
Toll like signaling pathway (TLR2, and pIKKα/β)
decreased in animals treated with grape juice when compared to cadmium
group.
Conclusion Taken together, we conclude that grape and apple juices had a
protective effect by means of antioxidant, antigenotoxic actions and for
promoting tissue regeneration in the kidney of rats following cadmium
intoxication.
Crown Procyanidin Tetramer: A Procyanidin with an Unusual Cyclic Skeleton with a Potent Protective Effect against Amyloid-β-Induced Toxicity
