High-level extracellular protein expression in Bacillus subtilis by optimizing strong promoters based on the transcriptome of Bacillus subtilis and Bacillus megaterium

2018 ◽  
Vol 151 ◽  
pp. 72-77 ◽  
Author(s):  
Xin Liu ◽  
Hai Wang ◽  
Bin Wang ◽  
Li Pan
Keyword(s):  
Bacillus Subtilis ◽  
Protein Expression ◽  
Bacillus Megaterium ◽  
Extracellular Protein ◽  
High Level

High-level exogenous glutamic acid-independent production of poly-(γ-glutamic acid) with organic acid addition in a new isolated Bacillus subtilis C10

2012 ◽  
Vol 116 ◽  
pp. 241-246 ◽  
Author(s):  
Huili Zhang ◽  
Jianzhong Zhu ◽  
Xiangcheng Zhu ◽  
Jin Cai ◽  
Anyi Zhang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Glutamic Acid ◽  
Organic Acid ◽  
Acid Addition ◽  
High Level

Productivity of forage grasses affected by microbial preparations in the Vologda region

2021 ◽  
pp. 21-25
Author(s):  
А.В. Платонов ◽  
И.И. Рассохина ◽  
Л.В. Сухарева ◽  
Г.Ю. Лаптев ◽  
В.Н. Большаков
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Megaterium ◽  
Nutritional Value ◽  
Crop Productivity ◽  
Exchange Energy ◽  
Lactobacillus Buchneri ◽  
Green Mass ◽  
Digestible Protein ◽  
Green Mass Yield ◽  
Feed Unit

Использование микробных препаратов — перспективный путь повышения продуктивности сельскохозяйственных растений. В статье приведены результаты изучения влияния биопрепаратов, созданных на основе живых штаммов микроорганизмов Bacillus subtilis («Натурост»), Lactobacillus buchneri («Натурост-Актив») и Bacillus megaterium («Натурост-М»), на продуктивность и питательную ценность райграса однолетнего и клеверо-тимофеечной смеси. Исследование проводили в мелкоделяночном полевом опыте во ФГБУН ВолНЦ РАН (Вологодская область) в 2019–2020 годах. Под влиянием обработки биопрепаратами выход зелёной массы райграса возрастал на 13,7–65,5% в зависимости от опытного варианта. Продуктивность травосмеси клевера и тимофеевки увеличилась на 13,1–46,6% в зависимости от используемого биопрепарата, укоса и года исследования. Оценка питательной ценности райграса показала, что обработка биопрепаратами способствовала повышению содержания кормовых единиц в сухом веществе на 6,5%, также несколько увеличилось содержание обменной энергии, сырого и переваримого протеина, сахаров и жиров. Питательная ценность клеверо-тимофеечной смеси по содержанию кормовых единиц под влиянием биопрепаратов увеличилась на 15%. В опытах с райграсом бόльшая продуктивность зелёной массы получена при использовании препарата «Натурост-Актив», в опытах с клеверо-тимофеечной смесью отмечена бόльшая эффективность препарата «Натурост». В исследованиях 2019 года повышение питательной ценности зелёной массы у обеих культур в большей степени происходило под влиянием препарата, созданного на основе бактерий Bacillus megaterium. В 2020 году более выраженное увеличение содержания кормовых единиц, обменной энергии, сырого протеина, переваримого протеина и жиров происходило при внесении препарата на основе бактерий Bacillus subtilis. Microbial preparations were shown to be promising when increasing crop productivity. This article reports on the effect of biopreparations containing living strains of Bacillus subtilis (“Naturost”), Lactobacillus buchneri (“Naturost-Aktiv”) and Bacillus megaterium (“Naturost-M”) on the yield and nutritional value of annual ryegrass and clover-timothy mixture. Microplot field trial took place in 2019–2020. Biopreparations improved green mass yield of ryegrass by 13.7–65.5%. The productivity of the clover-timothy mixture increased by 13.1–46.6% affected by biopreparations, cut and year. Treatment with biopreparations increased feed unit content by 6.5% in dry matter (DM) as well as exchange energy, crude and digestible protein, sugar and fat. Biopreparations improved feed unit content of the clover-timothy mixture by 15%. Ryegrass produced the highest yield of green mass under “Naturost-Aktiv” application, while “Naturost” was more effective for the clover-timothy mixture. In 2019 Bacillus megaterium had the best effect on the nutritional value of crop green mass. The contents of feed units, exchange energy, crude and digestible proteins as well as fat grew significantly after Bacillus subtilis application in 2020.

scholarly journals Analysis of the Bacillus subtilis spoIIIJ Gene and Its Paralogue Gene, yqjG

2002 ◽  
Vol 184 (7) ◽  
pp. 1998-2004 ◽  
Author(s):  
Takako Murakami ◽  
Koki Haga ◽  
Michio Takeuchi ◽  
Tsutomu Sato
Keyword(s):  
Bacillus Subtilis ◽  
Membrane Proteins ◽  
Vegetative Growth ◽  
Fluorescent Protein ◽  
Specific Expression ◽  
Rich Media ◽  
Green Fluorescent ◽  
High Level ◽  
Fluorescent Protein Reporter

ABSTRACT The Bacillus subtilis spoIIIJ gene, which has been proven to be vegetatively expressed, has also been implicated as a sporulation gene. Recent genome sequencing information in many organisms reveals that spoIIIJ and its paralogous gene, yqjG, are conserved from prokaryotes to humans. A homologue of SpoIIIJ/YqjG, the Escherichia coli YidC is involved in the insertion of membrane proteins into the lipid bilayer. On the basis of this similarity, it was proposed that the two homologues act as translocase for the membrane proteins. We studied the requirements for spoIIIJ and yqjG during vegetative growth and sporulation. In rich media, the growth of spoIIIJ and yqjG single mutants were the same as that of the wild type, whereas spoIIIJ yqjG double inactivation was lethal, indicating that together these B. subtilis translocase homologues play an important role in maintaining the viability of the cell. This result also suggests that SpoIIIJ and YqjG probably control significantly overlapping functions during vegetative growth. spoIIIJ mutations have already been established to block sporulation at stage III. In contrast, disruption of yqjG did not interfere with sporulation. We further show that high level expression of spoIIIJ during vegetative phase is dispensable for spore formation, but the sporulation-specific expression of spoIIIJ is necessary for efficient sporulation even at the basal level. Using green fluorescent protein reporter to monitor SpoIIIJ and YqjG localization, we found that the proteins localize at the cell membrane in vegetative cells and at the polar and engulfment septa in sporulating cells. This localization of SpoIIIJ at the sporulation-specific septa may be important for the role of spoIIIJ during sporulation.

scholarly journals Spo0A-Dependent Activation of an Extended −10 Region Promoter in Bacillus subtilis

2006 ◽  
Vol 188 (4) ◽  
pp. 1411-1418 ◽  
Author(s):  
Guangnan Chen ◽  
Amrita Kumar ◽  
Travis H. Wyman ◽  
Charles P. Moran
Keyword(s):  
Bacillus Subtilis ◽  
Base Pair ◽  
Promoter Activity ◽  
Mutational Analysis ◽  
Dna Binding Protein ◽  
Base Pairs ◽  
Dnase I Footprinting ◽  
Level Of Activity ◽  
High Level ◽  
Activity Dependent

ABSTRACT At the onset of endospore formation in Bacillus subtilis the DNA-binding protein Spo0A directly activates transcription from promoters of about 40 genes. One of these promoters, Pskf, controls expression of an operon encoding a killing factor that acts on sibling cells. AbrB-mediated repression of Pskf provides one level of security ensuring that this promoter is not activated prematurely. However, Spo0A also appears to activate the promoter directly, since Spo0A is required for Pskf activity in a ΔabrB strain. Here we investigate the mechanism of Pskf activation. DNase I footprinting was used to determine the locations at which Spo0A bound to the promoter, and mutations in these sites were found to significantly reduce promoter activity. The sequence near the −10 region of the promoter was found to be similar to those of extended −10 region promoters, which contain a TRTGn motif. Mutational analysis showed that this extended −10 region, as well as other base pairs in the −10 region, is required for Spo0A-dependent activation of the promoter. We found that a substitution of the consensus base pair for the nonconsensus base pair at position −9 of Pskf produced a promoter that was active constitutively in both ΔabrB and Δspo0A ΔabrB strains. Therefore, the base pair at position −9 of Pskf makes its activity dependent on Spo0A binding, and the extended −10 region motif of the promoter contributes to its high level of activity.

scholarly journals In Vivo and In Vitro evaluation of the efficacy of a peracetic acid-based disinfectant for decontamination of acrylic resins

2006 ◽  
Vol 17 (2) ◽  
pp. 117-121 ◽  
Author(s):  
Ana Lúcia Campani Chassot ◽  
Maria Inês Pereira Poisl ◽  
Susana Maria Werner Samuel
Keyword(s):  
Bacillus Subtilis ◽  
Peracetic Acid ◽  
Bacillus Stearothermophilus ◽  
Acrylic Resin ◽  
Human Saliva ◽  
Acrylic Resins ◽  
The Media ◽  
High Level

The purpose of this study was to assess the antimicrobial efficacy of a peracetic acid-based disinfectant for decontamination of heat-polymerized, chemically activated and microwave-polymerized acrylic resins. Resin plates were contaminated in vivo upon intraoral use by 10 volunteers for 7 nights and slabs were contaminated in vitro by contact with Bacillus subtilis and Bacillus stearothermophilus. The contaminated acrylic resin specimens were immersed in a 0.2% peracetic acid-based disinfectant (Sterilife®; Lifemed) for 5 min or 10 min and placed in a BHI culture medium. After incubation at 37°C for 48 h, bacterial growth was assessed by analyzing turbidity of the medium. For all types of acrylic resin, no turbidity of the medium was observed for any of the resin specimens immersed in the peracetic acid-based disinfectant for either 5 or 10 min. On the other hand, the media with specimens that were not immersed in the disinfectant (control) showed turbidity in 100% of the cases, indicating the presence of microorganisms in both tested conditions. In conclusion, immersion for at least 5 min in a 0.2% peracetic acid-based disinfectant promoted high-level disinfection of heat-polymerized, chemically activated and microwave-polymerized acrylic resins contaminated with either human saliva or Bacillus subtilis or Bacillus stearothermophilus.

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