Antifungal and defense elicitor activity of potassium phosphite against fungal blast disease on ptxD-OE transgenic indica rice and its acceptor parent

AbstractRice plays an important role in feeding the world’s population, specially the people who live in developing countries. Rice occupies one third of the world’s crops and provides 35 to 60 percent of calories consumed by 2.7 billion people in the world. More than 90 percent of world’s rice is produced and consumed in Asia. Blast disease caused by Magnaporthe Oryzae is one of the most important diseases of rice in the world. This study was evaluated the Power of ten blast specific SSR Markers in determination genetic diversity between thirty cultivars of Indica rice. The phenotypic evaluation was also performed using Magnaporthe Oryzae in stage of three or four leaves. The total of 23 effective alleles with an average of 2.21 alleles per locus affecting microsatellite markers were amplified in genotypes. The total of 28 alleles was also observed per locus with 2.8 average. Also, the average of PIC was estimated 42.0 for markers. The RM204 marker has the maximum of PIC (0.60) and RM413 marker has the minimum of PIC (0.081). The calculated similarities result between molecular data by Jacard similarity coefficient and UPGMA algorithm have divided thirty studied genotypes into three categories. This analysis between coordinates decides the cluster analysis group. In phenotypic testing, the calculated similarities between phenotypic has also occurred using coefficient similarity Dist and UPGMA algorithm. According to phenotypic evaluation the population was also divided into three groups. According to T-Student test for comparing the phenotypic and genotypic data, SSR markers "RM277" and “RM8225” were linked to semi-susceptible, and resistant phenotype.

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Association Analysis for Yield and Quality Attributes in Indica Rice and Screening of Hybrids Against Blast Disease (Magnaporthe grisea Barr.)

Journal of Plant Sciences ◽

10.3923/jps.2013.45.56 ◽

2013 ◽

Vol 8 (2) ◽

pp. 45-56 ◽

Cited By ~ 3

Author(s):

Mukesh Kumar Singh ◽

Prakash Singh ◽

Ravi P. Singh . ◽

Chinmayee Mohapatra .

Keyword(s):

Association Analysis ◽

Magnaporthe Grisea ◽

Indica Rice ◽

Quality Attributes ◽

Blast Disease ◽

Yield And Quality

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Molecular Mapping of the New Blast Resistance Genes Pi47 and Pi48 in the Durably Resistant Local Rice Cultivar Xiangzi 3150

Phytopathology ◽

10.1094/phyto-08-10-0209 ◽

2011 ◽

Vol 101 (5) ◽

pp. 620-626 ◽

Cited By ~ 33

Author(s):

Hongmei Huang ◽

Ling Huang ◽

Guangping Feng ◽

Suhua Wang ◽

Yue Wang ◽

...

Keyword(s):

Molecular Mapping ◽

Blast Resistance ◽

Indica Rice ◽

Recombinant Inbred Lines ◽

Rice Cultivar ◽

Blast Disease ◽

Hunan Province ◽

R Genes ◽

Chromosome 11 ◽

High Level

The indica rice cultivar Xiangzi 3150 (XZ3150) confers a high level of resistance to 95% of the isolates of Magnaporthe oryzae (the agent of rice blast disease) collected in Hunan Province, China. To identify the resistance (R) gene(s) controlling the high level of resistance in this cultivar, we developed 286 F9 recombinant inbred lines (RILs) from a cross between XZ3150 and the highly susceptible cultivar CO39. Inoculation of the RILs and an F2 population from a cross between the two cultivars with the avirulent isolate 193-1-1 in the growth chamber indicated the presence of two dominant R genes in XZ3150. A linkage map with 134 polymorphic simple sequence repeat and single feature polymorphism markers was constructed with the genotype data of the 286 RILs. Composite interval mapping (CIM) using the results of 193-1-1 inoculation showed that two major R genes, designated Pi47 and Pi48, were located between RM206 and RM224 on chromosome 11, and between RM5364 and RM7102 on chromosome 12, respectively. Interestingly, the CIM analysis of the four resistant components of the RILs to the field blast population revealed that Pi47 and Pi48 were also the major genetic factors responsible for the field resistance in XZ3150. The DNA markers linked to the new R genes identified in this study should be useful for further fine mapping, gene cloning, and marker-aided breeding of blast-resistant rice cultivars.

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