Indacaterol, glycopyrronium, and mometasone: pharmacological interaction and anti-inflammatory profile in hyperresponsive airways

Abstract Background To investigate whether an exercise intervention using the VIVIFRAIL© protocol has benefits for inflammatory and functional parameters in different frailty status. Methods/design This is a randomized clinical trial in an outpatient geriatrics clinic including older adults ≥60 years. For each frailty state (frail, pre-frail and robust), forty-four volunteers will be randomly allocated to the control group (n = 22) and the intervention group (n = 22) for 12 weeks. In the control group, participants will have meetings of health education while those in the intervention group will be part of a multicomponent exercise program (VIVIFRAIL©) performed five times a week (two times supervised and 3 times of home-based exercises). The primary outcome is a change in the inflammatory profile (a reduction in inflammatory interleukins [IL-6, TNF- α, IL1beta, IL-17, IL-22, CXCL-8, and IL-27] or an increase in anti-inflammatory mediators [IL-10, IL1RA, IL-4]). Secondary outcomes are change in physical performance using the Short Physical Performance Battery, handgrip strength, fatigue, gait speed, dual-task gait speed, depressive symptoms, FRAIL-BR and SARC-F scores, and quality of life at the 12-week period of intervention and after 3 months of follow-up. Discussion We expect a reduction in inflammatory interleukins or an increase in anti-inflammatory mediators in those who performed the VIVIFRAIL© protocol. The results of the study will imply in a better knowledge about the effect of a low-cost intervention that could be easily replicated in outpatient care for the prevention and treatment of frailty, especially regarding the inflammatory and anti-inflammatory pathways involved in its pathophysiology. Trial registration Brazilian Registry of Clinical Trials (RBR-9n5jbw; 01/24/2020). Registred January 2020. http://www.ensaiosclinicos.gov.br/rg/RBR-9n5jbw/.

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In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium

Medicines ◽

10.3390/medicines6040101 ◽

2019 ◽

Vol 6 (4) ◽

pp. 101 ◽

Cited By ~ 2

Author(s):

Cholet ◽

Decombat ◽

Vareille-Delarbre ◽

Gainche ◽

Berry ◽

...

Keyword(s):

Mononuclear Cells ◽

Polymorphonuclear Neutrophils ◽

Ros Production ◽

Peripheral Blood Mononuclear ◽

Aerial Parts ◽

Bupleurum Scorzonerifolium Willd ◽

Anti Inflammatory ◽

Inflammatory Profile ◽

Bupleurum Scorzonerifolium

Background: Some Bupleurum species, such as the Bupleurum chinense DC. or the Bupleurum scorzonerifolium Willd have been extensively studied (especially their roots) for the treatment of inflammation. In contrast, only compounds extracted from the aerial parts of Bupleurum rotundifolium have been studied and showed anti-inflammatory or antiproliferative activities. This study was conducted to investigate the antioxidant, anti-inflammatory, and immunomodulatory effects of Bupleurum rotundifolium roots. Methods: To tackle the various aspects of inflammation, we studied in vitro a methanolic extract from the roots of Bupleurum rotundifolium on peripheral blood mononuclear cells (PBMCs), polymorphonuclear neutrophils (PMNs), and the monocytic cells THP-1. Its antioxidant capacities and iron-chelating activity were assessed. The extract was tested on THP-1 differentiation, reactive oxygen species (ROS) production by leukocytes, neutrophils chemotaxis, cytokines, PGE2 production, and NF-κB activation in PBMCs. Results: The extract showed a decreased ROS production in stimulated cells. It increased PBMC chemokine secretion and up-regulated the differentiation of THP-1 monocytes into macrophage-like cells, indicating a potential interest of the extract in the resolution of acute inflammation. In addition, the analysis of cytokine production suggests that Bupleurum rotundifolium has immunomodulatory properties. Conclusions: Cytokines secretion, especially IL-1β and IL-12p70, provided us with a set of indicators suggesting that the extract might be able to drive the polarization of macrophages and lymphocytes toward a Th2 anti-inflammatory profile in excessive inflammation.

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Extra Virgin Olive Oil Extracts Modulate the Inflammatory Ability of Murine Dendritic Cells Based on Their Polyphenols Pattern: Correlation between Chemical Composition and Biological Function

Antioxidants ◽

10.3390/antiox10071016 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1016

Author(s):

Stefania De Santis ◽

Marina Liso ◽

Giulio Verna ◽

Francesca Curci ◽

Gualtiero Milani ◽

...

Keyword(s):

Dendritic Cells ◽

Olive Oil ◽

Virgin Olive Oil ◽

Adaptive Immune Response ◽

Extra Virgin Olive Oil ◽

Pattern Correlation ◽

Important Health ◽

Health Promoting ◽

Anti Inflammatory ◽

Inflammatory Profile

Extra virgin olive oil (EVOO) represents one of the most important health-promoting foods whose antioxidant and anti-inflammatory activities are mainly associated to its polyphenols content. To date, studies exploring the effect of EVOO polyphenols on dendritic cells (DCs), acting as a crosstalk between the innate and the adaptive immune response, are scanty. Therefore, we studied the ability of three EVOO extracts (cv. Coratina, Cima di Mola/Coratina, and Casaliva), characterized by different polyphenols amount, to regulate DCs maturation in resting conditions or after an inflammatory stimulus. Cima di Mola/Coratina and Casaliva extracts were demonstrated to be the most effective in modulating DCs toward an anti-inflammatory profile by reduction of TNF and IL-6 secretion and CD86 expression, along with a down-modulation of Il-1β and iNOS expression. From factorial analysis results, 9 polyphenols were tentatively established to play a synergistic role in modulating DCs inflammatory ability, thus reducing the risk of chronic inflammation.

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New chemical constituents from the fruits of Zanthoxylum armatum and its in vitro anti-inflammatory profile

Natural Product Research ◽

10.1080/14786419.2017.1405404 ◽

2017 ◽

Vol 33 (5) ◽

pp. 665-672 ◽

Cited By ~ 6

Author(s):

Zulfa Nooreen ◽

Anant Kumar ◽

Dnyaneshwar Umrao Bawankule ◽

Sudeep Tandon ◽

Mohd Ali ◽

...

Keyword(s):

Chemical Constituents ◽

Zanthoxylum Armatum ◽

Anti Inflammatory ◽

Inflammatory Profile

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Progesterone and VIP cross-talk enhances phagocytosis and anti-inflammatory profile in trophoblast-derived cells

Molecular and Cellular Endocrinology ◽

10.1016/j.mce.2017.01.022 ◽

2017 ◽

Vol 443 ◽

pp. 146-154 ◽

Cited By ~ 8

Author(s):

Daiana Vota ◽

Mariana Aguero ◽

Esteban Grasso ◽

Vanesa Hauk ◽

Lucila Gallino ◽

...

Keyword(s):

Cross Talk ◽

Anti Inflammatory ◽

Inflammatory Profile

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The influence of surface modified poly(l-lactic acid) films on the differentiation of human monocytes into macrophages

Biomaterials Science ◽

10.1039/c6bm00920d ◽

2017 ◽

Vol 5 (3) ◽

pp. 551-560 ◽

Cited By ~ 9

Author(s):

Clara R. Correia ◽

Joana Gaifem ◽

Mariana B. Oliveira ◽

Ricardo Silvestre ◽

João F. Mano

Keyword(s):

Lactic Acid ◽

Macrophage Polarization ◽

Human Monocytes ◽

Anti Inflammatory ◽

Inflammatory Profile ◽

Surface Modified

The interaction of human monocytes with different surface modified poly(l-lactic acid) films was evaluated. All surface modified films disrupted the balance of macrophage polarization towards a favorable anti-inflammatory profile, particularly after an LPS stimulus.

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PPAR-Gamma Agonist Pioglitazone Reduced CD68+ but Not CD163+ Macrophage Dermal Infiltration in Obese Psoriatic Patients

PPAR Research ◽

10.1155/2020/4548012 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Ya. O. Yemchenko ◽

V. I. Shynkevych ◽

K. Ye Ishcheikin ◽

I. P. Kaidashev

Keyword(s):

Immunohistochemical Study ◽

Ppar Gamma ◽

Obese Patients ◽

Before And After ◽

Anti Inflammatory ◽

Inflammatory Profile ◽

Skin Morphology ◽

Macrophage Populations ◽

Inflammatory Action ◽

M1 And M2 Macrophages

Background. Macrophages are of great importance in the development of obesity and psoriasis. Signaling via PPAR-γ in certain macrophage populations is associated with M2-like features and anti-inflammatory profile. In this research, we evaluated the anti-inflammatory action of pioglitazone by the immunohistochemical study of M1 and M2 macrophages in psoriasis-affected skin in obese patients. Methods. We used immunohistochemistry to characterize CD68+ and CD163+ macrophages and pathomorphological description of skin biopsy, obtained from 6 obese psoriatic patients before and after treatment with 15, 30, and 45 mg pioglitazone, once a day during 6 months. Two patients with conventional therapy and without pioglitazone served as control. Results. Generally, CD163+ cell quantities in psoriasis-affected skin significantly dominated over CD68+ before and after all treatment regiments. Among patients who received pioglitazone, some of them clearly responded to treatment from lowest to highest doses by decreasing CD68+ cells. In the group with 30 mg pioglitazone regiment, we detected a significant reduction of CD68+ cells in dermal infiltrates: CI 95% (16–32) before versus CI 95% (2–7) after treatment. Pioglitazone dose escalation led to certain normalization of skin morphology. Conclusion. The immunohistochemical study allows us to show the anti-inflammatory effect of pioglitazone in psoriatic obese patients, which can be mediated by reducing the number of СD68+ macrophages, but not СD163+ macrophages, in the affected dermis.

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Obesity Affects β2 Adrenergic Regulation of the Inflammatory Profile and Phenotype of Circulating Monocytes from Exercised Animals

Nutrients ◽

10.3390/nu11112630 ◽

2019 ◽

Vol 11 (11) ◽

pp. 2630 ◽

Cited By ~ 5

Author(s):

Isabel Gálvez ◽

Leticia Martín-Cordero ◽

María Dolores Hinchado ◽

Alberto Álvarez-Barrientos ◽

Eduardo Ortega

Keyword(s):

Inflammatory Response ◽

Adrenergic Receptor ◽

Acute Exercise ◽

Inflammatory Responses ◽

Adrenergic Regulation ◽

Immune Stress ◽

Β2 Adrenergic Receptor ◽

Anti Inflammatory ◽

Inflammatory Profile ◽

Inflammatory Effect

Anomalous immune/inflammatory responses in obesity take place along with alterations in the neuroendocrine responses and dysregulation in the immune/stress feedback mechanisms. Exercise is a potential anti-inflammatory strategy in this context, but the influence of exercise on the β2 adrenergic regulation of the monocyte-mediated inflammatory response in obesity remains completely unknown. The first objective of this study was to analyze the effect of exercise on the inflammatory profile and phenotype of monocytes from obese and lean animals, and the second aim was to determine whether obesity could affect monocytes’ inflammatory response to β2 adrenergic activation in exercised animals. C57BL/6J mice were allocated to different lean or obese groups: sedentary, with acute exercise, or with regular exercise. The inflammatory profile and phenotype of their circulating monocytes were evaluated by flow cytometry in the presence or absence of the selective β2 adrenergic receptor agonist terbutaline. Exercise caused an anti-inflammatory effect in obese individuals and a pro-inflammatory effect in lean individuals. β2 adrenergic receptor stimulation exerted a global pro-inflammatory effect in monocytes from exercised obese animals and an anti-inflammatory effect in monocytes from exercised lean animals. Thus, β2 adrenergic regulation of inflammation in monocytes from exercised animals seems to depend on the inflammatory basal set-point.

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