Bioactive metabolites from the desert plant-associated endophytic fungus Chaetomium globosum (Chaetomiaceae)

In the current study, an ethyl acetate extract from the endophytic fungus Aspergillus sp. SPH2 isolated from the stem parts of the endemic plant Bethencourtia palmensis was screened for its biocontrol properties against plant pathogens (Fusarium moniliforme, Alternaria alternata, and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi), plant parasites (Meloidogyne javanica), and ticks (Hyalomma lusitanicum). SPH2 gave extracts with strong fungicidal and ixodicidal effects at different fermentation times. The bioguided isolation of these extracts gave compounds 1–3. Mellein (1) showed strong ixodicidal effects and was also fungicidal. This is the first report on the ixodicidal effects of 1. Neoaspergillic acid (2) showed potent antifungal effects. Compound 2 appeared during the exponential phase of the fungal growth while neohydroxyaspergillic acid (3) appeared during the stationary phase, suggesting that 2 is the biosynthetic precursor of 3. The mycotoxin ochratoxin A was not detected under the fermentation conditions used in this work. Therefore, SPH2 could be a potential biotechnological tool for the production of ixodicidal extracts rich in mellein.

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Antifungal metabolites from Chaetomium globosum, an endophytic fungus in Ginkgo biloba

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2011.06.019 ◽

2011 ◽

Vol 39 (4-6) ◽

pp. 876-879 ◽

Cited By ~ 43

Author(s):

Hu-Qiang Li ◽

Xiao-Jun Li ◽

You-Lin Wang ◽

Qiang Zhang ◽

An-Ling Zhang ◽

...

Keyword(s):

Ginkgo Biloba ◽

Endophytic Fungus ◽

Chaetomium Globosum ◽

Antifungal Metabolites

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Optimization of cultural conditions affecting improved bioactive metabolite production by endophytic fungus Trichoderma harzianum

Bionatura ◽

10.21931/rb/2021.06.04.8 ◽

2021 ◽

Vol 6 (4) ◽

pp. 2187-2192

Author(s):

Rashid Rahim Hateet ◽

Zainab Alag Hassan ◽

Abdulameer Abdullah Al-Mussawi ◽

Shaima Rabeea Banoon

Keyword(s):

Antibacterial Activity ◽

Trichoderma Harzianum ◽

Endophytic Fungus ◽

Nitrogen Sources ◽

Bioactive Metabolites ◽

Bioactive Metabolite ◽

Metabolite Production ◽

Surface Sterilization ◽

Cultural Conditions

The present study aimed to optimize cultural conditions for optimum bioactive metabolite production by endophytic fungus Trichoderma harzianum, isolated by surface sterilization method from the leaf of the eucalyptus plant. The fungus was identified based on morphological characterization. Fungal metabolites were carried out by ethyl acetate solvent. The antibacterial activity was tested against Escherichia coli (ATCC 25922) and Staphylococcus aureus (NCTC 6571). Various carbon, nitrogen sources, pH, temperature, incubation period, and NaCl on the antibacterial metabolite production were studied. Bioactive metabolite production of T. harzianum exhibits a broad spectrum of in vitro antibacterial activity against two strains of bacteria. For the optimum production of bioactive metabolites, Dextrose and Glucose were found to be the best sources of carbon and the best sources of Nitrogen Yeast extract (YE) and (NH4)2SO. The maximum production of bioactive metabolites occurs at pH 7 and 25°C.; the NaCl showed a positive influence on bioactive metabolites.

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Secondary Metabolites of the Endophytic Fungus Chaetomium globosum Isolated From Coptis chinensis

Natural Product Communications ◽

10.1177/1934578x211044574 ◽

2021 ◽

Vol 16 (9) ◽

pp. 1934578X2110445

Author(s):

Jia-Cheng Ji ◽

Pan-Pan Wei ◽

Xiao-Yang Han ◽

Zheng-Hui Li ◽

Hong-Lian Ai ◽

...

Keyword(s):

Endophytic Fungus ◽

Early Childhood Development ◽

Childhood Development ◽

Chaetomium Globosum ◽

Resonance Spectroscopy ◽

Ionization Mass ◽

Two Dimensional ◽

Coptis Chinensis ◽

Relative Configuration ◽

One Dimensional

A new steroid, chaetglotone (1), together with 3 known compounds (2-4), were isolated from Chaetomium globosum, which is an endophytic fungus isolated from the root of Coptis chinensis Franch . The new compound was characterized by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy and high-resolution electrospray ionization mass spectrometer. The relative configuration and absolute configuration of 1 were further determined via the DP4 + and Early Childhood Development protocols, separately.

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Bioactive Metabolites Produced by the Endophytic Fungus Phomopsis sp. YM355364

Natural Product Communications ◽

10.1177/1934578x1400900521 ◽

2014 ◽

Vol 9 (5) ◽

pp. 1934578X1400900 ◽

Cited By ~ 1

Author(s):

Kai-Xia Ma ◽

Xin-Tian Shen ◽

Rong Huang ◽

Tang Wang ◽

Xiao-Song Xie ◽

...

Keyword(s):

Spectral Analysis ◽

Inhibitory Activity ◽

Endophytic Fungus ◽

Influenza A ◽

Pathogenic Fungi ◽

Bioactive Metabolites ◽

Antifungal Activities ◽

Aconitum Carmichaeli

A new compound, 16-acetoxycytosporone B (1), along with four known ones, dankasterone A (2), dankasterone B (3), 3β,5α,9α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (4), and cyclonerodiol oxide (5), were isolated from Phomopsis sp. YM355364, an endophytic fungus of Aconitum carmichaeli. Their structures were characterized by spectral analysis. Compound 2 exhibited significant inhibitory activity against influenza A/Thailand/Kan353/2004(H5N1) pseudovirus with an IC50 value of 3.56 μM. Compounds 1, 2, and 4 showed either moderate or weak antifungal activities against four pathogenic fungi.

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