Characterization of the PRODUCTION of ANTHOCYANIN PIGMENT 1 Arabidopsis dominant mutant using DLEMMA dual isotope labeling approach

2021 ◽  
Vol 186 ◽  
pp. 112740
Author(s):  
Yonghui Dong ◽  
Liron Feldberg ◽  
Ilana Rogachev ◽  
Asaph Aharoni
Keyword(s):  
Isotope Labeling ◽  
Anthocyanin Pigment ◽  
Dual Isotope ◽  
Dominant Mutant ◽  
Labeling Approach

scholarly journals A dual-isotope-labeling method of studying the bioavailability of hexaglutamyl folic acid relative to that of monoglutamyl folic acid in humans by using multiple orally administered low doses

2006 ◽  
Vol 84 (5) ◽  
pp. 1128-1133 ◽  
Author(s):  
Alida Melse-Boonstra ◽  
Petra Verhoef ◽  
Clive E West ◽  
Johannes A van Rhijn ◽  
Richard B van Breemen ◽  
...  
Keyword(s):  
Folic Acid ◽  
Isotope Labeling ◽  
Low Doses ◽  
Dual Isotope ◽  
Labeling Method

scholarly journals Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control

2014 ◽  
Vol 207 (4) ◽  
pp. 463-480 ◽  
Author(s):  
Jonathan Bizarro ◽  
Christophe Charron ◽  
Séverine Boulon ◽  
Belinda Westman ◽  
Bérengère Pradet-Balade ◽  
...  
Keyword(s):  
Isotope Labeling ◽  
Core Protein ◽  
3D Structure ◽  
Assembly Mechanism ◽  
Core Proteins ◽  
Assembly Factors ◽  
Assembly Factor

In vitro, assembly of box C/D small nucleolar ribonucleoproteins (snoRNPs) involves the sequential recruitment of core proteins to snoRNAs. In vivo, however, assembly factors are required (NUFIP, BCD1, and the HSP90–R2TP complex), and it is unknown whether a similar sequential scheme applies. In this paper, we describe systematic quantitative stable isotope labeling by amino acids in cell culture proteomic experiments and the crystal structure of the core protein Snu13p/15.5K bound to a fragment of the assembly factor Rsa1p/NUFIP. This revealed several unexpected features: (a) the existence of a protein-only pre-snoRNP complex containing five assembly factors and two core proteins, 15.5K and Nop58; (b) the characterization of ZNHIT3, which is present in the protein-only complex but gets released upon binding to C/D snoRNAs; (c) the dynamics of the R2TP complex, which appears to load/unload RuvBL AAA+ adenosine triphosphatase from pre-snoRNPs; and (d) a potential mechanism for preventing premature activation of snoRNP catalytic activity. These data provide a framework for understanding the assembly of box C/D snoRNPs.

scholarly journals NMR Spectroscopic Characterization of the C‐Mannose Conformation in a Thrombospondin Repeat Using a Selective Labeling Approach

2020 ◽  
Vol 132 (46) ◽  
pp. 20840-20846
Author(s):  
Hendrik R. A. Jonker ◽  
Krishna Saxena ◽  
Aleksandra Shcherbakova ◽  
Birgit Tiemann ◽  
Hans Bakker ◽  
...  
Keyword(s):  
Spectroscopic Characterization ◽  
Selective Labeling ◽  
Labeling Approach

D2O-Isotope-Labeling Approach to Probing Phosphate-Solubilizing Bacteria in Complex Soil Communities by Single-Cell Raman Spectroscopy

2019 ◽  
Vol 91 (3) ◽  
pp. 2239-2246 ◽  
Author(s):  
Hong-Zhe Li ◽  
Qing-fang Bi ◽  
Kai Yang ◽  
Bang-Xiao Zheng ◽  
Qiang Pu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Single Cell ◽  
Isotope Labeling ◽  
Phosphate Solubilizing Bacteria ◽  
Soil Communities ◽  
Labeling Approach ◽  
Phosphate Solubilizing

Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)

2016 ◽  
Vol 133 ◽  
pp. 100-112 ◽  
Author(s):  
Victor Llombart ◽  
Teresa García-Berrocoso ◽  
Joan Josep Bech-Serra ◽  
Alba Simats ◽  
Alejandro Bustamante ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Stable Isotope ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Isotope Labeling ◽  
Vitro Model

scholarly journals In vivo characterization of the novel CD44v6-targeting Fab fragment AbD15179 for molecular imaging of squamous cell carcinoma: a dual-isotope study

2014 ◽  
Vol 4 (1) ◽  
pp. 11 ◽  
Author(s):  
Anna-Karin Haylock ◽  
Diana Spiegelberg ◽  
Johan Nilvebrant ◽  
Karl Sandström ◽  
Marika Nestor
Keyword(s):  
Squamous Cell Carcinoma ◽  
Molecular Imaging ◽  
Cell Carcinoma ◽  
The Novel ◽  
Fab Fragment ◽  
Dual Isotope ◽  
Isotope Study ◽  
In Vivo Characterization

scholarly journals Genetic Localization and Molecular Characterization of the nonS Gene Required for Macrotetrolide Biosynthesis inStreptomyces griseus DSM40695

2000 ◽  
Vol 44 (7) ◽  
pp. 1809-1817 ◽  
Author(s):  
Wyatt C. Smith ◽  
Longkuan Xiang ◽  
Ben Shen
Keyword(s):  
Polyketide Synthase ◽  
Isotope Labeling ◽  
Streptomyces Griseus ◽  
Open Reading Frames ◽  
A Cell ◽  
Cyclic Polyethers ◽  
The Difference ◽  
Reading Frames

ABSTRACT The macrotetrolides are a family of cyclic polyethers derived from tetramerization, in a stereospecific fashion, of the enantiomeric nonactic acid (NA) and its homologs. Isotope labeling experiments established that NA is of polyketide origin, and biochemical investigations demonstrated that 2-methyl-6,8-dihydroxynon-2E-enoic acid can be converted into NA by a cell-free preparation from Streptomyces lividans that expresses nonS. These results lead to the hypothesis that macrotetrolide biosynthesis involves a pair of enantiospecific polyketide pathways. In this work, a 55-kb contiguous DNA region was cloned from Streptomyces griseus DSM40695, a 6.3-kb fragment of which was sequenced to reveal five open reading frames, including the previously reported nonR andnonS genes. Inactivation of nonS in vivo completely abolished macrotetrolide production. Complementation of thenonS mutant by the expression of nonS intrans fully restored its macrotetrolide production ability, with a distribution of individual macrotetrolides similar to that for the wild-type producer. In contrast, fermentation of thenonS mutant in the presence of exogenous (±)-NA resulted in the production of nonactin, monactin, and dinactin but not in the production of trinactin and tetranactin. These results prove the direct involvement of nonS in macrotetrolide biosynthesis. The difference in macrotetrolide production between in vivo complementation of the nonS mutant by the plasmid-borne nonSgene and fermentation of the nonS mutant in the presence of exogenously added (±)-NA suggests that NonS catalyzes the formation of (−)-NA and its homologs, supporting the existence of a pair of enantiospecific polyketide pathways for macrotetrolide biosynthesis inS. griseus. The latter should provide a model that can be used to study the mechanism by which polyketide synthase controls stereochemistry during polyketide biosynthesis.

Characterization of an additional anthocyanin pigment in extracts of strawberries, Fragaria

1970 ◽  
Vol 50 ◽  
pp. 311-318 ◽  
Author(s):  
R.E. Wrolstad ◽  
K.I. Hildrum ◽  
J.F. Amos
Keyword(s):  
Anthocyanin Pigment

scholarly journals Characterization of an Antagonist Interleukin-6 Dimer by Stable Isotope Labeling, Cross-linking, and Mass Spectrometry

2002 ◽  
Vol 277 (48) ◽  
pp. 46487-46492 ◽  
Author(s):  
Thomas Taverner ◽  
Nathan E. Hall ◽  
Richard A. J. O'Hair ◽  
Richard J. Simpson
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Interleukin 6 ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Cross Linking
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