A mitogen-activated protein kinase, AhMPK6 from peanut localizes to the nucleus and also induces defense responses upon transient expression in tobacco

2010 ◽  
Vol 48 (6) ◽  
pp. 481-486 ◽  
Author(s):  
Koppolu Raja Rajesh Kumar ◽  
Pulugurtha Bharadwaja Kirti
Keyword(s):  
Protein Kinase ◽  
Transient Expression ◽  
Defense Responses ◽  
Mitogen Activated Protein Kinase ◽  
Mitogen Activated Protein

scholarly journals Identification of Msp1-Induced Signaling Components in Rice Leaves by Integrated Proteomic and Phosphoproteomic Analysis

2019 ◽  
Vol 20 (17) ◽  
pp. 4135 ◽  
Author(s):  
Ravi Gupta ◽  
Cheol Woo Min ◽  
Yu-Jin Kim ◽  
Sun Tae Kim
Keyword(s):  
Plasma Membrane ◽  
Protein Kinase ◽  
Defense Responses ◽  
Mitogen Activated Protein Kinase ◽  
Calcium Dependent ◽  
Molecular Pattern ◽  
Mitogen Activated Protein ◽  
Dependent Protein ◽  
Rice Leaves ◽  
Respiratory Burst Oxidase

MSP1 is a Magnaporthe oryzae secreted protein that elicits defense responses in rice. However, the molecular mechanism of MSP1 action is largely elusive. Moreover, it is yet to be established whether MSP1 functions as a pathogen-associated molecular pattern (PAMP) or an effector. Here, we employed a TMT-based quantitative proteomic analysis of cytosolic as well as plasma membrane proteins to decipher the MSP1 induced signaling in rice. This approach led to the identification of 6691 proteins, of which 3049 were identified in the plasma membrane (PM), while 3642 were identified in the cytosolic fraction. A parallel phosphoproteome analysis led to the identification of 1906 phosphopeptides, while the integration of proteome and phosphoproteome data showed activation of proteins related to the proteolysis, jasmonic acid biosynthesis, redox metabolism, and MAP kinase signaling pathways in response to MSP1 treatment. Further, MSP1 induced phosphorylation of some of the key proteins including respiratory burst oxidase homologue-D (RBOHD), mitogen-activated protein kinase kinase kinase-1 (MEKK1), mitogen-activated protein kinase-3/6 (MPK3/6), calcium-dependent protein kinase (CDPK) and calmodulin (CaM) suggest activation of PAMP-triggered immunity (PTI) in response to MSP1 treatment. In essence, our results further support the functioning of MSP1 as a PAMP and provide an overview of the MSP1 induced signaling in rice leaves.

scholarly journals Genome-Wide Transcriptomic Analysis Reveals Insights into the Response to Citrus bark cracking viroid (CBCVd) in Hop (Humulus lupulus L.)

Viruses ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 570 ◽  
Author(s):  
Ajay Mishra ◽  
Atul Kumar ◽  
Deepti Mishra ◽  
Vishnu Nath ◽  
Jernej Jakše ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Sugar Metabolism ◽  
Defense Responses ◽  
Mitogen Activated Protein Kinase ◽  
Sequencing Analysis ◽  
Severe Stunting ◽  
Expression Of Genes ◽  
Pathogenesis Related Protein ◽  
Genes Encoding ◽  
Mitogen Activated Protein

Viroids are smallest known pathogen that consist of non-capsidated, single-stranded non-coding RNA replicons and they exploits host factors for their replication and propagation. The severe stunting disease caused by Citrus bark cracking viroid (CBCVd) is a serious threat, which spreads rapidly within hop gardens. In this study, we employed comprehensive transcriptome analyses to dissect host-viroid interactions and identify gene expression changes that are associated with disease development in hop. Our analysis revealed that CBCVd-infection resulted in the massive modulation of activity of over 2000 genes. Expression of genes associated with plant immune responses (protein kinase and mitogen-activated protein kinase), hypersensitive responses, phytohormone signaling pathways, photosynthesis, pigment metabolism, protein metabolism, sugar metabolism, and modification, and others were altered, which could be attributed to systemic symptom development upon CBCVd-infection in hop. In addition, genes encoding RNA-dependent RNA polymerase, pathogenesis-related protein, chitinase, as well as those related to basal defense responses were up-regulated. The expression levels of several genes identified from RNA sequencing analysis were confirmed by qRT-PCR. Our systematic comprehensive CBCVd-responsive transcriptome analysis provides a better understanding and insights into complex viroid-hop plant interaction. This information will assist further in the development of future measures for the prevention of CBCVd spread in hop fields.

scholarly journals Genome-wide transcriptomic analysis reveals insights into the response to Citrus bark cracking viroid (CBCVd) in hop (Humulus lupulus L.)

2018 ◽  
Author(s):  
Ajay Kumar Mishra ◽  
Atul Kumar ◽  
Deepti Mishra ◽  
Vishnu Sukumari Nath ◽  
Jernej Jakse ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Sugar Metabolism ◽  
Defense Responses ◽  
Mitogen Activated Protein Kinase ◽  
Sequencing Analysis ◽  
Severe Stunting ◽  
Expression Of Genes ◽  
Pathogenesis Related Protein ◽  
Genes Encoding ◽  
Mitogen Activated Protein

Viroids are smallest pathogen that consist of non-capsidated, single-stranded non-coding RNA replicons and exploits host factors for their replication and propagation. The severe stunting disease caused by Citrus bark cracking viroid (CBCVd) is a serious threat, which spread rapidly within hop gardens. In this study, we employed comprehensive transcriptome analyses to dissect host-viroid interactions and identify gene expression changes associated with disease development in hop. Our analysis revealed that CBCVd-infection resulted in the massive modulation of activity of over 2000 genes. Expression of genes associated with plant immune responses (protein kinase and mitogen-activated protein kinase), hypersensitive responses, phytohormone signaling pathways, photosynthesis, pigment metabolism, protein metabolism, sugar metabolism and modification and others were altered, which could be attributed to systemic symptom development upon CBCVd-infection in hop. In addition, genes encoding RNA-dependent RNA polymerase, pathogenesis-related protein, chitinase as well as those related to basal defense responses were up-regulated. The expression levels of several genes identified from RNA sequencing analysis were confirmed by qRT-PCR. Our systematic comprehensive CBCVd-responsive transcriptome analysis provides a better understanding and insights into complex viroid-hop plant interaction. This information will assist further in the development of future measures for the prevention of CBCVd spread in hop fields.

scholarly journals Agroinfiltration by Cytokinin-Producing Agrobacterium sp. Strain GV3101 Primes Defense Responses in Nicotiana tabacum

2014 ◽  
Vol 27 (11) ◽  
pp. 1175-1185 ◽  
Author(s):  
Arsheed Hussain Sheikh ◽  
Badmi Raghuram ◽  
Lennart Eschen-Lippold ◽  
Dierk Scheel ◽  
Justin Lee ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Protein Kinase ◽  
Pseudomonas Syringae ◽  
Defense Response ◽  
Mapk Pathway ◽  
Defense Responses ◽  
Mitogen Activated Protein Kinase ◽  
In Planta ◽  
Mitogen Activated Protein ◽  
Higher Doses

Transient infiltrations in tobacco are commonly used in plant studies, but the host response to different disarmed Agrobacterium strains is not fully understood. The present study shows that pretreatment with disarmed Agrobacterium tumefaciens GV3101 primes the defense response to subsequent infection by Pseudomonas syringae in Nicotiana tabacum. The presence of a trans-zeatin synthase (tzs) gene in strain GV3101 may be partly responsible for the priming response, as the tzs-deficient Agrobacterium sp. strain LBA4404 only weakly imparts such responses. Besides inducing the expression of defense-related genes like PR-1 and NHL10, GV3101 pretreatment increased the expression of tobacco mitogen-activated protein kinase (MAPK) pathway genes like MEK2, WIPK (wound-induced protein kinase), and SIPK (salicylic acid-induced protein kinase). Furthermore, the GV3101 strain showed a stronger effect than the LBA4404 strain in activating phosphorylation of the tobacco MAPK, WIPK and SIPK, which presumably prime the plant immune machinery. Lower doses of exogenously applied cytokinins increased the activation of MAPK, while higher doses decreased the activation, suggesting a balanced level of cytokinins is required to generate defense response in planta. The current study serves as a cautionary warning for plant researchers over the choice of Agrobacterium strains and their possible consequences on subsequent pathogen-related studies.

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