A stress-related transcription factor belonging to the YL-1 family is differently regulated in durum wheat cultivars differing in drought sensitivity

AbstractThe cloning of agriculturally important genes is often complicated by haplotype variation across crop cultivars. Access to pan-genome information greatly facilitates the assessment of structural variations and rapid candidate gene identification. Here, we identified the red glume 1 (Rg-B1) gene using association genetics and haplotype analyses in ten reference grade wheat genomes. Glume color is an important trait to characterize wheat cultivars. Red glumes are frequent among Central European spelt, a dominant wheat subspecies in Europe before the 20th century. We used genotyping-by-sequencing to characterize a global diversity panel of 267 spelt accessions, which provided evidence for two independent introductions of spelt into Europe. A single region at the Rg-B1 locus on chromosome 1BS was associated with glume color in the diversity panel. Haplotype comparisons across ten high-quality wheat genomes revealed a MYB transcription factor as candidate gene. We found extensive haplotype variation across the ten cultivars, with a particular group of MYB alleles that was conserved in red glume wheat cultivars. Genetic mapping and transient infiltration experiments allowed us to validate this particular MYB transcription factor variants. Our study demonstrates the value of multiple high-quality genomes to rapidly resolve copy number and haplotype variations in regions controlling agriculturally important traits.

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The durum wheat NAC transcription factor TtNAC2A enhances drought stress tolerance in Arabidopsis

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2021.104439 ◽

2021 ◽

Vol 186 ◽

pp. 104439

Author(s):

Dhawya Mergby ◽

Moez Hanin ◽

Mohammed Najib Saidi

Keyword(s):

Transcription Factor ◽

Drought Stress ◽

Durum Wheat ◽

Stress Tolerance ◽

Nac Transcription Factor ◽

Drought Stress Tolerance

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Detection of deleted in malignant brain tumors 1 and runt-related transcription factor 3 gene expressions in bladder carcinoma

Molecular Biology Reports ◽

10.1007/s11033-011-1261-9 ◽

2011 ◽

Vol 39 (4) ◽

pp. 4691-4695 ◽

Cited By ~ 4

Author(s):

Yavuz Dodurga ◽

Çığır Biray Avcı ◽

N. Lale Satiroglu-Tufan ◽

Canten Tataroglu ◽

Zehra Kesen ◽

...

Keyword(s):

Transcription Factor ◽

Brain Tumors ◽

Bladder Carcinoma ◽

Gene Expressions ◽

Malignant Brain Tumors ◽

Related Transcription Factor ◽

Transcription Factor 3

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Synergistic attenuation of ovariectomy-induced bone loss by combined use of fish oil and 17β-oestradiol

British Journal Of Nutrition ◽

10.1017/s0007114517000344 ◽

2017 ◽

Vol 117 (4) ◽

pp. 479-489 ◽

Cited By ~ 4

Author(s):

Youri Jin ◽

Myoungsook Lee ◽

Yongsoon Park

Keyword(s):

Transcription Factor ◽

Bone Loss ◽

Protective Efficacy ◽

Recovery Period ◽

Serum Levels ◽

Protective Mechanism ◽

Beneficial Effects ◽

Combined Use ◽

Related Transcription Factor ◽

Turnover Markers

AbstractOestrogen and n-3 PUFA, especially EPA and DHA, have been reported to have beneficial effects on bone loss. Thus, the purpose of the present study was to investigate the synergistic bone-protective mechanism of combined treatments of EPA+DHA supplementation and oestrogen injection in ovariectomised rats. Rats were fed a modified American Institute of Nutrition-93G diet with 0 %, 1 % or 2 % n-3 PUFA (EPA+DHA) relative to the total energy intake for 12 weeks. Rats were surgically ovariectomised at week 8, and after a 1-week recovery period rats were injected with either 17β-oestradiol-3-benzoate (E2) or maize oil for the last 3 weeks. Combined use of n-3 PUFA and E2 synergistically increased femoral cortical bone volume, bone mineral content and the bone expression of runt-related transcription factor 2 (RUNX2), but decreased the bone expression of IL-1β. Both n-3 PUFA and E2 decreased the bone expressions of IL-7, TNF-α and PPAR-γ, and increased the bone expression of oestrogen receptor-α. n-3 PUFA in the presence of E2 and E2 alone significantly decreased the bone expressions of IL-1β and IL-6 and increased the bone expression of RUNX2. E2 significantly decreased the serum levels of bone turnover markers and the bone expression of receptor activator of NF-κB ligand, but decreased the bone expression of osteoprotegerin. The combined use of n-3 PUFA and E2 exerted synergistic bone-protective efficacy through up-regulation of RUNX2, an essential transcription factor for bone formation, as well as the suppression of bone-resorbing cytokine IL-1β.

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Alteration in the expression of MGMT and RUNX3 due to non-CpG promoter methylation and their correlation with different risk factors in esophageal cancer patients

Tumor Biology ◽

10.1177/1010428317701630 ◽

2017 ◽

Vol 39 (5) ◽

pp. 101042831770163 ◽

Cited By ~ 5

Author(s):

Snigdha Saikia ◽

Asad Ur Rehman ◽

Prajjalendra Barooah ◽

Preeti Sarmah ◽

Mallika Bhattacharyya ◽

...

Keyword(s):

Esophageal Cancer ◽

Transcription Factor ◽

Promoter Methylation ◽

Messenger Rna ◽

Dna Methyltransferase ◽

Rna Expression ◽

Betel Nut ◽

Methylguanine Dna Methyltransferase ◽

Related Transcription Factor ◽

Transcription Factor 3

Promoter methylation reflects in the inactivation of different genes like O6-methylguanine-DNA methyltransferase DNA repair gene and runt-related transcription factor 3, a known tumor suppressor gene in various cancers such as esophageal cancer. The promoter methylation was evaluated for O6-methylguanine-DNA methyltransferase and runt-related transcription factor 3 in CpG, CHH, and CHG context (where H is A, T, or C) by next-generation sequencing. The methylation status was correlated with quantitative messenger RNA expression. In addition, messenger RNA expression was correlated with different risk factors like tobacco, alcohol, betel nut consumption, and smoking habit. CpG methylation of O6-methylguanine-DNA methyltransferase promoter had a positive association in the development of esophageal cancer (p < 0.05), whereas runt-related transcription factor 3 promoter methylation showed no significant association (p = 1.0) to develop esophageal cancer. However, the non-CpG methylation, CHH, and CHG were significantly correlated with O6-methylguanine-DNA methyltransferase (p < 0.05) and runt-related transcription factor 3 (p < 0.05) promoters in the development of esophageal cancer. The number of cytosine converted to thymine (C→T) in O6-methylguanine-DNA methyltransferase promoter showed a significant correlation between cases and controls (p < 0.05), but in runt-related transcription factor 3 no such significant correlation was observed. Besides, messenger RNA expression was found to be significantly correlated with promoter hypermethylation of O6-methylguanine-DNA methyltransferase and runt-related transcription factor 3 in the context of CHG and CHH (p < 0.05). The CpG hypermethylation in O6-methylguanine-DNA methyltransferase showed positive (p < 0.05) association, whereas in runt-related transcription factor 3, it showed contrasting negative association (p = 0.23) with their messenger RNA expression. Tobacco, betel nut consumption, and smoking habits were associated with altered messenger RNA expression of O6-methylguanine-DNA methyltransferase (p < 0.05) and betel nut consumption and smoking habits were associated with runt-related transcription factor 3 (p < 0.05). There was no significant association between messenger RNA expression of O6-methylguanine-DNA methyltransferase and runt-related transcription factor 3 with alcohol consumption (p = 0.32 and p = 0.15). In conclusion, our results suggest that an aberrant messenger RNA expression may be the outcome of CpG, CHG, and CHH methylation in O6-methylguanine-DNA methyltransferase, whereas outcome of CHG and CHH methylation in runt-related transcription factor 3 promoters along with risk factors such as consumption of tobacco, betel nut, and smoking habits in esophageal cancer from Northeast India.

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