Blood Fatty Acid Analysis Reveals Similar n-3 Fatty Acid Composition in Non-Pregnant and Pregnant Women and Their Neonates in an Israeli Pilot Study

Author(s):  
Alicia Leikin-Frenkel ◽  
Aya Mohr–Sasson ◽  
Matan Anteby ◽  
Michal Kandel-Kfir ◽  
Ayelet Harari ◽  
...  
2017 ◽  
Vol 4 (04) ◽  
Author(s):  
SUNITA SINGH ◽  
R. P. SINGH ◽  
H. K. SINGH ◽  
N. A. KHAN ◽  
M. K. MAURYA

Among the oilseed Brassica crops, Indian mustard [Brassica juncea (L.) Czern and Coss.] is an important source of oil from a nutritional point of view. The nutritional value of oil and cake quality is governed mainly by the composition of its fatty acids, iodine value, saponification, acid value, glucosinolates, crude fibre, protein and limiting amino acids, etc. Seventeen varieties/strains of Indian mustard were taken for saturated and unsaturated fatty acid analysis. The eicosenoic was absent in genotype (NUDBYJ-10) and erucic acid (NUDBYJ-10, LES-46 and Pusa mustard- 21). The fatty acid composition found a variable in different genotypes. Saturated fatty acid, Palmitic + Stearic ranged between 2.3 to 6.5%, Oleic 10.6 to 40.7%, Linoleic 16.1 to 37.7%, Linolenic 13.3 to 26.7%, Eicosenoic 0.00 to 10.30% and Erucic acid 0.00 to 47.50%, respectively. Alternaria blight severity also varied in different genotypes and ranged between 18.75 to 56.25%, maximum being in genotype Kranti and minimum in LES-47. No significant correlation was observed between the fatty acid composition and disease severity. The oil content range from 38.1 to 42.60% and protein content was found highest in variety RGN-73. The amino acid viz. methionine and tryptophan range between 0.41 to 1.81 g/16gN and 0.41 to 1.81 g /16g N, respectively.


1990 ◽  
Vol 70 (4) ◽  
pp. 1041-1051 ◽  
Author(s):  
T. A. VAN LUNEN ◽  
R. L. WILSON ◽  
L. M. POSTE ◽  
G. BUTLER

Seventy-two feeder pigs, with a mean weight of 24.6 kg, were randomly allotted in groups of three to four dietary treatments to determine the effect of feeding poultry offal hydrolysate (POH) on fatty acid composition and meat quality. The four dietary treatments were: 0% POH (control), 5% POH, 10% POH and 15% POH on a dry matter basis. At slaughter (96.8 kg), the right loins from 60 pigs were retained for fatty acid analysis and meat quality evaluation. The longissimus dorsi muscle was dissected from each loin; 200 g were stored in an atmosphere of nitrogen and frozen for fatty acid analysis and 500 g were frozen for meat quality evaluation. Fat was extracted and methylated for fatty acid analysis. Separation and identification of individual fatty acid methyl esters was performed using gas chromatography. Sensory meat evaluation (pork flavour, off-flavour, tenderness and juiciness) was performed by 10 trained panelists. Warner-Bratzler shear evaluation and free moisture analyses were performed. The fatty acids in pork appear to exhibit a sensitive response to changes in dietary fat. Linear trends for fatty acids in the pork were, for the most part, opposite to trends in the diets. Inclusion of POH resulted in an increase in the total polyunsaturates present in the pork. There was no effect (P > 0.05) due to dietary treatment on flavour, tenderness or juiciness of the pork. No significant differences were found among the Warner-Bratzler shear measurements or free moisture determinations. Changes in fatty acid profile of pork do not appear to affect eating quality when POH is included in the diet at levels of up to 15% DM. Key words: Poultry offal, pork, fatty acid, meat quality


2018 ◽  
Vol 58 (5) ◽  
pp. 828 ◽  
Author(s):  
Khaled Kanakri ◽  
Beverly Muhlhausler ◽  
John Carragher ◽  
Robert Gibson ◽  
Reza Barekatain ◽  
...  

Manipulation of the fatty acid composition of chicken feed has been shown to be effective for improving the nutritional value of chicken products. Currently, however, evaluation of the effectiveness of this approach requires invasive blood sampling or post mortem tissue sampling of the birds. Preen oil can be collected non-invasively from live birds. So this study aimed to test the hypothesis that the fatty acid composition of preen oil reflects that of the blood. Male and female meat chickens (Cobb 500) were fed a diet supplemented with 4% (w/w) flaxseed oil (high n-3 polyunsaturates) or beef tallow (mostly monounsaturates and saturates) for 6 weeks. Preen oil and whole blood samples (n = 9 birds per sex/diet treatment group) were collected freshly post mortem for fatty acid analysis. Preen oil analysis showed that ~97% of fatty acids were saturates, with a small percentage of n-6 polyunsaturates and traces of other types. There were negligible n-3 polyunsaturates in preen oil. Proportions of some saturated fatty acids were slightly, but significantly, affected by diet (C16:0 (P < 0.05) and C17:0 (P < 0.01)) or by gender (C10:0 and C18:0) (P < 0.05). Some fatty acids with odd numbers of carbon atoms (e.g. C17:0 and C19:0) were found in relatively high concentrations in preen oil, despite not being detectable in either the diet or blood. In conclusion, the fatty acid composition of preen oil does not accurately reflect the fatty acid profile of the blood; it is not, therefore, a suitable alternative for determining fatty acid status of meat chickens.


Author(s):  
Zuzanna Goluch ◽  
Artur Rybarczyk ◽  
Zuzanna Goluch ◽  
Gabriela Haraf ◽  
Andrzej Okruszek ◽  
...  

2011 ◽  
Vol 26 (1) ◽  
pp. 61-69
Author(s):  
Milan Ivanovic ◽  
Katarina Gasic ◽  
Andjelka Calic ◽  
Nemanja Kuzmanovic ◽  
Mirko Ivanovic ◽  
...  

Automated method of fatty acid analysis was used to identify and study heterogeneity of 41 Erwinia amylovora strains, originating from 8 plant species grown in 13 locations in Serbia and one in Montenegro. All strains contained 14:0 3OH fatty acid, characteristic for the ?amylovora? group. According to fatty acid composition 39 strains were identified as E. amylovora as the first choice from the database. Due to their specific fatty acid composition, two strains were identified as E. amylovora, but as a second choice. Fatty acid analysis also showed that E. amylovora population from Serbia could be differentiated in three groups, designated in this study as ?, ? and ?. All strains originating from central or south Serbia, as well as four strains from north Serbia clustered into group ?. Group ? and ? contained only strains isolated in northern Serbia (Vojvodina). The results show that E. amylovora population in this area is heterogeneous and indicate pathogen introduction from different directions. Fatty acid analysis enabled identification at species level, as well as new insights of heterogeneity of E. amylovora population.


2011 ◽  
Vol 15 (1) ◽  
pp. 274 ◽  
Author(s):  
Julius Pontoh ◽  
Nancy T.N Buyung

ANALISA ASAM LEMAK DALAM MINYAK KELAPA MURNI (VCO) DENGAN DUA PERALATAN KROMATOGRAFI GAS Julius Pontoh1) dan Nancy T.N. Buyung2); e-mail:[email protected] 1)Program Studi Kimia FMIPA Universitas Sam Ratulangi, Manado 95115 2)Alumni Program Studi Kimia FMIPA Universitas Sam Ratulangi, Manado 95119 ABSTRAK Komposisi asam asam lemak dalam minyak kelapa sangat penting untuk menilai kualitas dari minyak tersebut.  Untuk minyak kelapa, komposisi dari asam lemak rantai menengah seperti kaprilik, kaprat dan laurat menjadi asam asam lemak penting.  Di Indonesia ada dua laboratorium yang banyak digunakan untuk menganalisa asam asam lemak dalam minyak kelapa murni.  Tujuan penelitian ini adalah untuk membandingkan kedua laboratorium tersebut dalam menganalisa asam asam lemak.  Kromatogram dari Laboratorium pertama menunjukan garis dasar yang ebih baik, tetapi tidak dapat mendeteksi asam stearat.  Waktu retensi asam asam lemak dalam kedua komatogram sangat berbeda.  Demikian juga dengan luas puncak dari asam asam lemak berbeda dalam kedua kromatogram.  Namun demikian, persentasi luas pencak dari masing masing asam lemak dalam kedua kromatogram hampir sama. Kata kunci: kromatogarafi gas, waktu retensi   FATTY ACID ANALYSIS IN VIRGIN COCONUT OIL (VCO) WITH TWO TYPES GAS CHROMATOGRAPHY ABSTRACT Fatty acid composition in coconut oil is very important to evaluate the quality of the oil. For coconut oil, the composition of medium length of fatty acids such as caprylic, capric and lauric acids are the interest of the oil. To date, this fatty acid composition is the best to be analyzed by gas chromatography.  In Indonesia, there are two laboratories used to analyze the fatty acids.  The purpose of this study is to compare the two laboratories in the analysis the compounds.  Samples of commercial coconut oils were treated with acid and base to converted into Fatty acid methyl ester.  The derivatives were extracted with hexane and ready to be send to the laboratories.  The results show the chromatogram of the two laboratories is totally difference. The chromatograph from Laboratory 1 showed very good base line but there was no stearic fatty acid peak shown  The retention time for the same fatty acids is different. Peak areas among the same fatty acids are totally different between the two laboratories, but the percentage for each fatty acid is almost the same. Keywords: gas chromatography, retention time


1993 ◽  
Vol 20 (1) ◽  
pp. 9-11 ◽  
Author(s):  
W. L. Zeile ◽  
D. A. Knauft ◽  
C. B. Kelly

Abstract Modification of fatty acid composition can improve the keeping quality of peanut oil and may expand markets for peanut usage. Modification may involve hybridization to recombine existing genes, or the creation of new variability through mutagenesis or transformation with genes from other organisms. Identification of the fatty acid composition of individual seed could improve the chances of obtaining peanut genotypes with desired fatty acid composition. Published techniques for fatty acid analysis of individual peanut seed require the use of approximately half the cotyledonary seed tissue and utilize a process of solvent extraction and esterification. We have used a procedure that requires a small fraction of seed tissue and analyzes fatty acids through direct transmethylation. Comparisons were made between procedures using seven genotypes representing a wide range of fatty acid composition variability. Quantities of fatty acids with C&lt;20 were not statistically different between tests. While some differences were observed in longer-chained fatty acids (C≥20), the relative values among genotypes were similar. This procedure maintains greater integrity of the seed for planting purposes, requires less time and cost for fatty acid analysis, and can improve efficiency of individual seed analysis.


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