scholarly journals Grafting Cucumber onto Interspecific Cucurbita Hybrid Rootstocks to Improve Productivity and Control wilt Disease Caused by Fusarium oxysporum f. sp. cucumerinum

2021 ◽  
Vol 12 (1) ◽  
pp. 41-47
Author(s):  
S. Kamel ◽  
Dalia Taher
Keyword(s):  
Fusarium Oxysporum ◽  
Wilt Disease ◽  
And Control

scholarly journals Effect of Two Bioformulation Trichoderma harzianum and Pseudomonas fluorescens with Manure in Controlling Fusarium wilt Disease in Pumpkin

2019 ◽  
Vol 27 (1) ◽  
pp. 446-456
Author(s):  
Dhia S. Al–Waily ◽  
Sabreen M. Hassan
Keyword(s):  
Methyl Ester ◽  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Contaminated Soil ◽  
Trichoderma Harzianum ◽  
Octanoic Acid ◽  
Wilt Disease ◽  
Hexadecanoic Acid ◽  
Butanoic Acid ◽  
And Control

          This research aimed  to isolate and identified the pathogen caused wilt in pumpkin plants and control by two bioformulation  Trichoderma harzianum and  Pseudomonas fluorescens.           The results revealed  that the caused pathogen of pumpkin wilt plants was Fusarium oxysporum  which  diagnosis has been confirmed by using PCR Technique.           The results  of pathogenesity revealed that 60.17% from Plants grown in contaminated soil infected with F. oxysporum. Also the results showed that use the T. harzianum that has high antagonism with pathogen, the degree of antagonism has reached 2 with F. oxysporum at Bell scale. P. fluorescens showed inhibition ratio against F. oxysporum and T. harzianum. reached 44 and  27% respectively. The results with use antibiosis for T. harzianum , P. fluroescens and manure revealed that the least severity was in the transactions MThPf (manure, fungi and Bacteria), MTh (manure and fungi), ThPf (fungi and bacteria), Th (T. harzianum), Pf (P. fluorescens), MPf (manure and bacteria), where ranged 0 - 5.56% , which did not differ significantly between them compared to M (manure) and control that reached 13.69% and 22.22% respectively. Weight in soil contaminated with pathogen in MThPf and ThPf that reached 376.83 and 379.22gr compard with control in the same soil when reached 66.41 gr .The results of the analysis with used GCMas revealed transactions in presence of Antibiotics and substances Phenol 2,5-bis (1,1-dimethyl), 4-Fluorobenzyl alcohol, Hexadecanoic acid (2) -methyl ester, 1-Pyrrolidine butanoic acid, gamma-Tocophenol and 9-Octanoic acid (2) -2-hydrox.

scholarly journals Effect of Two Bioformulation Trichoderma harzianum and Pseudomonas fluorescens with Manure in Controlling Fusarium wilt Disease in Pumpkin

2019 ◽  
Vol 27 (1) ◽  
pp. 446-456
Author(s):  
Dhia S. Al – Waily ◽  
Sabreen M. Hassan
Keyword(s):  
Methyl Ester ◽  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Contaminated Soil ◽  
Trichoderma Harzianum ◽  
Octanoic Acid ◽  
Wilt Disease ◽  
Hexadecanoic Acid ◽  
Butanoic Acid ◽  
And Control

This research aimed  to isolate and identified the pathogen caused wilt in pumpkin plants and control by two bioformulation  Trichoderma harzianum and  Pseudomonas fluorescens.           The results revealed  that the caused pathogen of pumpkin wilt plants was Fusarium oxysporum  which  diagnosis has been confirmed by using PCR Technique.           The results  of pathogenesity revealed that 60.17% from Plants grown in contaminated soil infected with F. oxysporum. Also the results showed that use the T. harzianum that has high antagonism with pathogen, the degree of antagonism has reached 2 with F. oxysporum at Bell scale. P. fluorescens showed inhibition ratio against F. oxysporum and T. harzianum. reached 44 and  27% respectively. The results with use antibiosis for T. harzianum , P. fluroescens and manure revealed that the least severity was in the transactions MThPf (manure, fungi and Bacteria), MTh (manure and fungi), ThPf (fungi and bacteria), Th (T. harzianum), Pf (P. fluorescens), MPf (manure and bacteria), where ranged 0 - 5.56% , which did not differ significantly between them compared to M (manure) and control that reached 13.69% and 22.22% respectively. Weight in soil contaminated with pathogen in MThPf and ThPf that reached 376.83 and 379.22gr compard with control in the same soil when reached 66.41 gr .The results of the analysis with used GCMas revealed transactions in presence of Antibiotics and substances Phenol 2,5-bis (1,1-dimethyl), 4-Fluorobenzyl alcohol, Hexadecanoic acid (2) -methyl ester, 1-Pyrrolidine butanoic acid, gamma-Tocophenol and 9-Octanoic acid (2) -2-hydrox.

Dynamical aspects of pine wilt disease and control measures

2021 ◽  
Vol 145 ◽  
pp. 110764
Author(s):  
Takasar Hussain ◽  
Adnan Aslam ◽  
Muhammad Ozair ◽  
Fatima Tasneem ◽  
J.F. Gómez-Aguilar
Keyword(s):  
Pine Wilt Disease ◽  
Wilt Disease ◽  
Control Measures ◽  
Pine Wilt ◽  
And Control

Vascular Wilt of Common Naranjilla (Solanum quitoense) Caused by Fusarium oxysporum in Ecuador

2001 ◽  
Vol 2 (1) ◽  
pp. 15 ◽  
Author(s):  
J. B. Ochoa ◽  
B. Yangari ◽  
V. Galarza ◽  
J. Fiallos ◽  
M. A. Ellis
Keyword(s):  
Fusarium Oxysporum ◽  
Wilt Disease ◽  
Vascular Wilt ◽  
Major Constraint ◽  
Vascular Wilt Disease

Farmers have generally abandoned production of “common naranjilla” in many areas mainly due to uncontrollable epidemics of an apparent vascular wilt disease. Naranjilla vascular wilt (NVW) and is currently the major constraint to the production of naranjilla in Ecuador, where losses due to NVW may reach up to 80%. Accepted for publication 13 September 2001. Published 18 September 2001.

scholarly journals Detection and Control of Fusarium oxysporum from Soft Rot in Dendrobium officinale by Loop-Mediated Isothermal Amplification Assays

Biology ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1136
Author(s):  
Caiyun Xiao ◽  
Rongyu Li
Keyword(s):  
Fusarium Oxysporum ◽  
Integrated Management ◽  
Elongation Factor ◽  
Lamp Assay ◽  
Soft Rot ◽  
Isothermal Amplification ◽  
Dendrobium Officinale ◽  
Control Effect ◽  
Loop Mediated Isothermal Amplification ◽  
And Control

Soft rot causing Fusarium oxysporum is one of the most destructive diseases of Dendrobium officinale Kimura et Migo in China that reduces D. officinale yield and quality. A key challenge for an integrated management strategy for this disease is the rapid and accurate detection of F. oxysporum on D. officinale. Therefore, a new loop-mediated isothermal amplification (LAMP) assay was developed for this purpose. In this study, the primers were selected and designed using the translation elongation factor-1α (TEF-1α) gene region as the target DNA sequence in order to screen the best system of reaction of LAMP to detect F. oxysporum through optimizing different conditions of the LAMP reaction, including time, temperature, concentrations of MgSO4, and concentrations of inner and outer primers. The optimized system was able to efficiently amplify the target gene at 62 °C for 60 min with 1.2 μM internal primers, 0.4 μM external primers, 7 mM Mg2+, and 5 fg/µL minimum detection concentration of DNA for F. oxysporum. The amplified products could be detected with the naked eye after completion of the reaction with SYBR green I. We were better able to control the effect of soft rot in D. officinale using fungicides following a positive test result. Additionally, the control effect of synergism combinations against soft rot was higher than 75%. Thus, LAMP assays could detect F. oxysporum in infected tissues of D. officinale and soils in field, allowing for early diagnosis of the disease.

scholarly journals Uji masa simpan pelet Trichoderma harzianum dan kemampuannya dalam menghambat perkembangan penyakit Layu Fusarium pada bibit tomat.

2018 ◽  
Vol 3 (2) ◽  
pp. 117-127
Author(s):  
Rizka Musfirah ◽  
Rina Sriwati ◽  
Tjut Chamzurni
Keyword(s):  
Vitamin A ◽  
Shelf Life ◽  
Fusarium Oxysporum ◽  
Solanum Lycopersicum ◽  
Plant Height ◽  
Incubation Period ◽  
Fusarium Wilt ◽  
Trichoderma Harzianum ◽  
Wilt Disease ◽  
Damping Off

Abstrak. Tomat (Solanum lycopersicum) merupakan salah satu komoditas pertanian yang ditanam secara luas di seluruh dunia, termasuk di Indonesia, karena memiliki rasa yang khas dan enak, juga memiliki nilai gizi seperti sumber vitamin A dan C yang sangat baik. Produksi tomat mengalami penurunan setiap tahun, salah satunya diakibatkan oleh organisme penganggu tanaman (OPT) yaitu patogen Fusarium oxysporum sehingga perlu dilakukan pengendalian hayati yaitu menggunakan Trichoderma harzianum dalam bentuk formulasi pelet yang praktis, efektif, dan efesien. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 6 perlakuan dengan 3 ulangan, setiap perlakuan terdiri dari 10 unit bibit tomat. Penelitian ini terdiri dari 6 perlakuan yaitu perlakuan A (masa simpan pelet T. harzianum  4 minggu), B (masa simpan pelet T. harzianum 3 minggu), C (masa simpan pelet T. harzianum 2 minggu), D (masa simpan pelet T. harzianum 1 minggu), E (masa simpan pelet T. harzianum 0 minggu), F (tanpa perlakuan pelet T. harzianum). Peubah yang diamati yaitu pre-emergence damping off, post-emergence damping off, masa inkubasi, persentase tanaman layu, tinggi tanaman, dan jumlah daun. Hasil penelitian menunjukkan bahwa pelet T. harzianum yang disimpan 4 minggu efektif dalam menghambat perkembangan penyakit layu fusarium seperti menunda masa inkubasi sampai 7 HSI, menekan pre-emergence damping off sampai 90%, post-emergence damping off 92,95%, serta mampu meningkatkan tinggi tanaman sampai 19,63 cm dan meningkatkan jumlah daun rata-rata 7 helai pada 35 HSI. (Storing Period of Trichoderma harzianum Pellets and its ability to Inhibit the development of Fusarium Wilt Disease on Tomato Seeds)Abstract. Tomato (Solanum lycopersicum) is one of the most widely grown commodities in the world, including Indonesia. It has a distinctively good taste and many nutritional value such as vitamin A and C. However, tomato production has decreased every year. One of the main cause is the attacks by pathogens, named Fusarium oxysporum. A Biological control is necessary and the use of Trichoderma harzianum in the form of pellets is recommended because of its effectiveness, efficiency and practical use. This research used a Completely Randomized Design (RAL) non-factorial consisted of 6 treatments with 3 replications, each treatment consisted of 10 units of tomato seedlings. The 6 treatments are named as treatment A (T. harzianum pellet saving 4 weeks), B (T. harzianum pellet saving period 3 weeks), C (shelf life of 2 weeks T. harzianum pellet), D (shelf life of pellet T harzianum 1 week), E (shelf life of pellet T. harzianum 0 weeks), and F (without T. harzianum pellet treatment). The variables observed in this study are pre-emergence damping off, post-emergence damping off, incubation period, the percentage of wilted plants, plant height, and the number of leaves. The results showed that pellets of T. harzianum stored 4 weeks effectively inhibiting the development of fusarium wilt disease such as delaying incubation period up to 7 HSI (Days After Incubation), suppressing the pre-emergence damping off up to 90% and post-emergence damping off to 92.95%, also able to increase the plant height up to 19.63 cm and increase the average leaf number of 7 strands at 35 HSI.

scholarly journals Efficiency of Seed Treatment for Control of Chick Pea Wilt Disease caused by Fusarium oxysporum F. sp. ciceri

2020 ◽  
Vol 9 (12) ◽  
pp. 3109-3115
Author(s):  
Ravi Prakash Mishra ◽  
Manjul Pandey ◽  
Vibhuti Gupta ◽  
Deepali Bakshi
Keyword(s):  
Fusarium Oxysporum ◽  
Seed Treatment ◽  
Wilt Disease ◽  
Chick Pea

scholarly journals EVALUASI KETAHANAN AKSESI KENAF (Hibiscus cannabinus L.) TERHADAP PENYAKIT LAYU Fusarium oxysporum SCHLECT

2020 ◽  
Vol 6 (2) ◽  
pp. 29
Author(s):  
GEMBONG DALMADIYO ◽  
CECE SUHARA ◽  
SUPRIYONO SUPRIYONO ◽  
SUDJINDRO SUDJINDRO
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Hibiscus Cannabinus ◽  
Resistant Variety ◽  
Fusarium Wilt Disease ◽  
Screen House

<p><strong>Evaluation on the resistance of kenaf accessions (Hibis¬ cus cannabinus /..) to Fusarium oxysporum Schlect</strong></p><p>Resistant variety is one of the most important components controlling of fusarium wilt disease on kenaf caused by Fusarium oxysporum Schlect. To ind out resistant variety an evaluation on kenaf accessions was conducted in the laboratory and screen house of Phytopathology, RITFC, Malang in June-December 1997. The results of the selection on 77 accessions showed that 41 accessions were highly resistant, 1 2 accessions were resistant, 7 accessions were moderate, 12 accessions were susceptible, and 5 accessions were highly susceptible Three resistant and highly resistant accessions were namely 85-9-73, DS/005 H, and FJ/004 He could inhibit F. oxysporum growth about 23.40- 32.43 mm and its discolorisation about 0.0-13.4%.</p>

scholarly journals Seleksi Bakteri Antagonis untuk Mengendalikan Layu Fusarium pada Tanaman Phalaenopsis

2013 ◽  
Vol 22 (3) ◽  
pp. 276 ◽  
Author(s):  
Ika Djatnika
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Antagonistic Bacteria ◽  
Plant Research Institute ◽  
Fusarium Spp ◽  
Human Environment ◽  
Bacterial Antagonists ◽  
Important Disease ◽  
Selection Of

<p>ABSTRAK. Layu Fusarium merupakan penyakit penting yang menjadi kendala dalam memproduksi tanaman anggrek. Untuk mengendalikannya,  petani masih menggunakan fungsida. Tanaman anggrek kerap ditampilkan sebagai hiasan yang dekat dengan lingkungan manusia, maka penggunaan pestisida perlu diperhatikan. Oleh karena itu sangat penting dicari cara pengendalian lainnya yang aman terhadap lingkungan, antara lain dengan  pengendalian hayati. Tujuan penelitian ialah mendapatkan isolat bakteri antagonis yang dapat mengendalikan layu Fusarium pada tanaman Phalaenopsis. Percobaan dilakukan di Laboratorium dan Rumah Kasa Balai Penelitian Tanaman Hias Segunung (1100 m dpl.) serta untuk  mikrob diisolasi dari lokasi tanaman hortikultura di Jawa Barat dan DKI Jakarta, mulai Bulan Januari sampai dengan Desember 2010. Penelitian meliputi isolasi Fusarium spp. sebagai patogen pada tanaman anggrek di beberapa lokasi, isolasi bakteri antagonis, uji kemangkusan bakteri terhadap pertumbuhan Fusarium spp. di laboratorium, dan uji kemangkusan bakteri antagonis terhadap layu Fusarium di rumah kasa. Hasil penelitian menunjukkan bahwa penyebab layu Fusarium pada tanaman Phalaenopsis ialah Fusarium oxysporum. Dari 154 isolat bakteri yang diisolasi dari lapangan, hanya ada tiga  isolat yaitu nomor B23, B 26, dan B37 yang dapat menekan pertumbuhan F. oxysporum pada media PDA. Sampai dengan pengamatan minggu ke-10 setelah inokulasi, ketiga bakteri tersebut masing-masing menekan jumlah tanaman yang terserang layu Fusarium, yaitu sebesar 46,9; 48,9; dan 65,3%, dan masing-masing menekan intensitas penyakit layu 50,5; 43,9; dan 55,1%.</p><p>ABSTRACT. Djatnika, I 2012. Selection of Antagonistic Bacteria for Controlling of Fusarium Wilt on Phalaenopsis Plants. Fusarium wilt is an important disease as constraint on production of orchid plants. The control of Fusarium wilt of orchids with fungicides often use by farmers. Orchid plants are often displayed as a decoration which is close to the human environment, so the application of pesticides have to get attention. It is therefore necessary to find another method that is safe for environments, such as using of biological control. The purpose of the study was to get isolates of  bacterial antagonists for controlling of Fusarium wilt of Phalaenopsis plants. The experiment was conducted at Laboratory and Screenhouse of Indonesian Ornamental Plant Research Institute, Segunung (1100 m asl.) and the microbes were isolated from horticultural area in West Java and DKI Jakarta since January until December 2010. The research comprised of isolation of Fusarium spp. from orchid plants in some location, isolation of bacterial antagonists, the effectiveness of the bacteria to suppress Fusarium spp. growth in laboratory, and the effectiveness of the bacteria to control Fusarium wilt on Phalaenopsis plants in the screenhouse. The results showed that the causal Fusarium wilt of Phalaenopsis plants was identified as Fusarium oxysporum. Three of 154 isolates of bacteria, i.e. isolates number of B23, B26, and B37, could suppress of F. oxysporum growth on PDA media. Observation up to 10 weeks after inoculation, the three bacteria could reduce the number of  plants attacked by Fusarium wilt , which were 46.9; 48.9; and  65.3% respectively, and each of them suppress wilt disease intensity 50.5, 43.9, and 55.1% respectively. <br /><br /></p>

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