CONSEQUENCES AND CONTROL OF LATENT FUSARIUM OXYSPORUM INFECTIONS IN TULIP BULBS

Soft rot causing Fusarium oxysporum is one of the most destructive diseases of Dendrobium officinale Kimura et Migo in China that reduces D. officinale yield and quality. A key challenge for an integrated management strategy for this disease is the rapid and accurate detection of F. oxysporum on D. officinale. Therefore, a new loop-mediated isothermal amplification (LAMP) assay was developed for this purpose. In this study, the primers were selected and designed using the translation elongation factor-1α (TEF-1α) gene region as the target DNA sequence in order to screen the best system of reaction of LAMP to detect F. oxysporum through optimizing different conditions of the LAMP reaction, including time, temperature, concentrations of MgSO4, and concentrations of inner and outer primers. The optimized system was able to efficiently amplify the target gene at 62 °C for 60 min with 1.2 μM internal primers, 0.4 μM external primers, 7 mM Mg2+, and 5 fg/µL minimum detection concentration of DNA for F. oxysporum. The amplified products could be detected with the naked eye after completion of the reaction with SYBR green I. We were better able to control the effect of soft rot in D. officinale using fungicides following a positive test result. Additionally, the control effect of synergism combinations against soft rot was higher than 75%. Thus, LAMP assays could detect F. oxysporum in infected tissues of D. officinale and soils in field, allowing for early diagnosis of the disease.

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The role of gum induced by Fusarium oxysporum Schlecht. Snyd. et Hans. f.sp. tulipae Apt. in tulip bulbs on growth and development of the pathogen

Plant Protection Science ◽

10.17221/10515-pps ◽

2017 ◽

Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽

pp. 432-435

Author(s):

A. Saniewska

Keyword(s):

Fusarium Oxysporum ◽

Growth And Development ◽

Mycelium Growth ◽

Tulip Bulbs ◽

Bacto Agar

It was showed that gums induced by Fusarium oxysporum f.sp. tulipae in tulip bulbs applied to mineral Czapek-Dox Broth with Bacto Agar (CzDA) medium, containing sucrose substantially stimulated abundant growth of mycelium and sporulation of the pathogen. Addition of arabinose, xylose and their mixture, sugars occurring in tulip gum polysaccharide, to CzDA medium caused that mycelium was very sparse and sporulation was only slightly stimulated by arabinose. Mycelium growth on mineral CzDA medium without sucrose, was poor and sparse but addition of gum to the medium caused formation of abundant mycelium and increased sporulation of the pathogen. It is possible that polysaccharide of tulip gum may act mainly as elicitor and partially as substrate in regulation of mycelium growth and sporulation of Fusarium oxysporum f.sp. tulipae.

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A POSSIBLE EXPLANATION OF VARIATIONS IN SUSCEPTIBILITY OF TULIP BULBS TO INFECTION BY FUSARIUM OXYSPORUM

Acta Horticulturae ◽

10.17660/actahortic.1971.23.35 ◽

1971 ◽

pp. 225-229 ◽

Cited By ~ 7

Author(s):

B.H.H. Bergman ◽

J.C.M. Beijersbergen

Keyword(s):

Fusarium Oxysporum ◽

Tulip Bulbs

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Fusarium Wilt: A New Threat to Florida Lettuce Production

EDIS ◽

10.32473/edis-hs1385-2020 ◽

2020 ◽

Vol 2020 (5) ◽

Author(s):

German Sandoya ◽

Jesse J. Murray ◽

Richard N. Raid ◽

Christian F. Miller

Keyword(s):

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Control Measures ◽

Agricultural Area ◽

Disease Symptoms ◽

Everglades Agricultural Area ◽

Production Areas ◽

And Control

Fusarium wilt of lettuce is a disease caused by the fungus Fusarium oxysporum f.sp. lactucae. The disease is present in all lettuce production areas worldwide. Recently this disease has been identified in Florida in localized fields. This new 4-page publication of the UF/IFAS Horticultural Sciences Department presents information about the disease, symptoms, and control measures useful to lettuce growers in Florida. Following these recommendations may help to avoid the spread of this fungus in muck soils at the Everglades Agricultural Area (EAA), where 90% of the lettuce in Florida is planted. Written by Germán V. Sandoya, Jesse J. Murray, Richard N. Raid, and Christian F. Miller.https://edis.ifas.ufl.edu/hs1385

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Grafting Cucumber onto Interspecific Cucurbita Hybrid Rootstocks to Improve Productivity and Control wilt Disease Caused by Fusarium oxysporum f. sp. cucumerinum

Journal of Plant Production ◽

10.21608/jpp.2021.152018 ◽

2021 ◽

Vol 12 (1) ◽

pp. 41-47

Author(s):

S. Kamel ◽

Dalia Taher

Keyword(s):

Fusarium Oxysporum ◽

Wilt Disease ◽

And Control

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Genome resources of four distinct pathogenic races within Fusarium oxysporum f. sp. vasinfectum that cause vascular wilt disease of cotton

Phytopathology ◽

10.1094/phyto-07-20-0298-a ◽

2020 ◽

Author(s):

Subodh K Srivastava ◽

Kurt Zeller ◽

James H Sobieraj ◽

Mark K Nakhla

Keyword(s):

Fusarium Oxysporum ◽

Gene Prediction ◽

Whole Genome Sequence ◽

Vascular Wilt ◽

Gene Prediction Program ◽

Public Health Agencies ◽

Prediction Program ◽

Methods Development ◽

Pathogenic Races ◽

And Control

Whole Genome Sequence (WGS) based identifications are being increasingly used by regulatory and public health agencies to facilitate the detection, investigation, and control of pathogens and pests. Fusarium oxysporum f. sp. vasinfectum (FOV) is a significant vascular wilt pathogen of cultivated cotton, and consists of several pathogenic races that are not each other’s closest phylogenetic relatives. We have developed WGS assemblies for isolates of race 1 (FOV1), race 4 (FOV4), race 5 (FOV5), and race 8 (FOV8) using a combination of Nanopore (MinION) and Illumina sequencing technology (Mi-Seq). This resulted in assembled contigs with more than 100X coverage for each of the FOV races and estimated genome sizes of FOV1 52 Mb, FOV4 68 Mb, FOV5 68 Mb and FOV8 55 Mb. The AUGUSTUS gene prediction program predicted 16,263 genes in FOV1, 20,259 genes in FOV4, 20,375 genes in FOV5 and 16,615 genes in FOV8. We were able to identify 525 genes unique to FOV1, 570 unique to FOV4, 1242 unique to FOV5 and 383 unique to FOV8. We expect that these findings will help in comparative genomics, and in the identification of unique genes as candidate targets for diagnostic marker and methods development to permit rapid differentiation of FOV subgroups.

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The effect of chitosan on limitation of growth and development of some pathogenic fungi for ornamental plants

Acta Agrobotanica ◽

10.5586/aa.2001.002 ◽

2013 ◽

Vol 54 (1) ◽

pp. 17-29 ◽

Cited By ~ 2

Author(s):

Alicja Saniewska

Keyword(s):

Fusarium Oxysporum ◽

Inhibitory Action ◽

Marked Effect ◽

Ornamental Plants ◽

Pathogenic Fungi ◽

Inhibitory Effect ◽

Crab Shell ◽

Necrotic Spots ◽

Tulip Bulbs

The inhibitory effect of crab-shell chitosan, medium (200-800 cps) and high molecular weight ( 800-2000 cps) (purchased from Sigma-Aldrich Chemicals) toward Alternaria alternata, Botrytis tulipae, Fiisarium oxysporum f. sp. callistephi, Fusarium oxysporum f. sp. tulipae, Phoma narcissi and Phoma poolensis was evaluated in vitro and in vivo. The chitosan evidently inhibited in vitro growth of all tested pathogens, with a marked effect at higher concentrations above 200 μg/cm3. Chitosan at a concentration of 1,25; 2,5 and 5,0 mg/cm3 didn't have inhibitory action in appearance of fungi growth on naturally contaminated Callistephus chinensis seeds. At the same concentrations, chitosan applied as bulb scales dressing of Hymenocallis narcissiflora bulbs, before inoculation or after inoculation with Phoma narcissi, inhibited the development of necrotic spots on scales. Chitosan used preventively or curatively at a concentrations of 1,25; 2,5 and 5,0 mg/cm3 indicated inhibitory effect on development of Fusarium oxysporum f. sp. tulipae on tulip bulbs. Chitosan at a concentration of 10 mg/cm3 applied preventively (first spray 12th June) was very effective in the control of Puccinia antirrhini on snapdragon in the field. The strongest inhibitory effect was observed on snapdragon treated 8 times at week intervals.

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Race and Virulence Determination of Fusarium oxysporum f. sp. cubense Isolates from Sidomulyo Village of Bantul, Yogyakarta

Jurnal Perlindungan Tanaman Indonesia ◽

10.22146/jpti.26283 ◽

2018 ◽

Vol 22 (1) ◽

pp. 72

Author(s):

Herika Novrelly Jayatri ◽

Christanti Sumardiyono ◽

Arif Wibowo

Keyword(s):

Fusarium Oxysporum ◽

Severity Index ◽

Duncan Multiple Range Test ◽

Specific Primer ◽

Randomized Design ◽

Completely Randomized Design ◽

And Control ◽

Race 4 ◽

Range Test

Banana is one of the important fruit crop in Village of Sidomulyo, Bantul, Yogyakarta. One of important diseases which become the constraint in development of banana is Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc). This fungus has high race diversity and virulence, so that it required early detection for prevention and control of disease. This experiment was aimed to figure out race and virulence of Foc isolates from Village of Sidomulyo, Bantul, Yogyakarta. The 13 tested isolates were isolates of PR11, PKJ20, RU20, PR30, AH40, PKJ40, A41, RB42, PR43, RU51, A60, RP60, and A80. Race was molecularly detected using two types of primers, i.e. General Foc primer FocEf3 and specific primer for race 4 (Foc-1/Foc-2). Virulence test was performed on banana seedlings of Ambon Kuning cultivar using Completely Randomized Design (CRD) with 14 treatments and 4 repetitons. The observed parameters were external and internal symptoms, calculation of disease severity index and disease intensity. Data were analyzed using variance and further test of Duncan Multiple Range Test (DMRT) at 5 % level. The results showed that all isolates were Foc and 9 of 13 isolates were grouped into race 4, i.e. A80, RP60, PR11, A41, AH40, PKJ40, PR30, RB42, and PR43. The highest and lowest virulences were consecutively expressed by PR30, RB42, RU51, RP60, PR43, PKJ40, PR11, A41, AH40, RU20, PKJ20, A60, and A80, with severity index on leaves and rhizomes ranging 1.61-2.91 and 2.25-7, respectively. IntisariPisang merupakan tanaman buah unggulan di Desa Sidomulyo Kecamatan Bambanglipuro, Kabupaten Bantul. Salah satu penyakit penting yang menjadi kendala dalam pengembangan pisang adalah layu fusarium yang disebabkan oleh jamur Fusarium oxysporum f. sp. cubense (Foc). Jamur ini memiliki keragaman ras dan virulensi yang tinggi, sehingga deteksi dini diperlukan untuk pencegahan dan pengendalian penyakit. Penelitian ini bertujuan untuk mengetahui ras dan virulensi isolat Foc asal Desa Sidomulyo, Kecamatan Bambanglipuro, Kabupaten Bantul. Isolat yang diuji sebanyak 13 isolat, yakni isolat PR11, PKJ20, RU20, PR30, AH40, PKJ40, A41, RB42, PR43, RU51, A60, RP60, dan A80. Pengujian ras secara molekuler dengan menggunakan dua jenis primer yakni primer Foc in general FocEf3 dan primer spesifik ras 4 Foc-1/Foc-2. Uji virulensi pada bibit kultivar ambon kuning dengan menggunakan Racangan Acak Lengkap (RAL) yang terdiri dari 14 perlakuan dan 4 ulangan. Parameter yang diamati berupa pengamatan gejala luar dan gejala dalam, penghitungan indeks keparahan penyakit dan intensitas penyakit. Analisis data menggunakan sidik ragam dan uji lanjut Duncan Multiple Range Test (DMRT) pada taraf 5 %. Hasil pengujian menunjukkan bahwa semua isolat merupakan isolat Foc dan dari 13 isolat yang digunakan terdapat 9 isolat yang merupakan ras 4 yakni isolat A80, RP60, PR11, A41, AH40, PKJ40, PR30, RB42, dan PR43. Isolat yang memiliki virulensi tertinggi sampai terendah berturut-turut adalah PR30, RB42, RU51, RP60, PR43, PKJ40, PR11, A41, AH40, RU20, PKJ20, A60, dan A80, dengan indeks keparahan pada daun berkisar 1,61−2,91 dan indeks keparahan pada bonggol 2,25−7.

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