Methyl p-coumarate inhibits black spot rot on jujube fruit through membrane damage and oxidative stress against Alternaria alternata

2018 ◽  
Vol 145 ◽  
pp. 230-238 ◽  
Author(s):  
Wusun Li ◽  
Shuzhi Yuan ◽  
Qianqian Li ◽  
Weina Sang ◽  
Jiankang Cao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Alternaria Alternata ◽  
Membrane Damage ◽  
Black Spot ◽  
Jujube Fruit ◽  
And Oxidative Stress

Protective effect of diosmetin on in vitro cell membrane damage and oxidative stress in cultured rat hepatocytes

Toxicology ◽  
1992 ◽  
Vol 73 (2) ◽  
pp. 179-189 ◽  
Author(s):  
Pia Villa ◽  
Dario Cova ◽  
Laura De Francesco ◽  
Amalia Guaitani ◽  
Giuseppina Palladini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Membrane ◽  
Protective Effect ◽  
Membrane Damage ◽  
Rat Hepatocytes ◽  
Cell Membrane Damage ◽  
Cultured Rat Hepatocytes ◽  
And Oxidative Stress

scholarly journals Synergistic Antimicrobial Activity by Light or Thermal Treatment and Lauric Arginate: Membrane Damage and Oxidative Stress

2019 ◽  
Vol 85 (17) ◽  
Author(s):  
Xu Yang ◽  
Rewa Rai ◽  
Cuong Nguyen Huu ◽  
Nitin Nitin
Keyword(s):  
Oxidative Stress ◽  
Antimicrobial Activity ◽  
Membrane Damage ◽  
Polymyxin B ◽  
Stress Generation ◽  
Content Type ◽  
Mild Heat ◽  
Log Reduction ◽  
Physical Treatments ◽  
And Oxidative Stress

ABSTRACT The need for more effective antimicrobials is critical for the food industry to improve food safety and reduce spoilage of minimally processed foods. The present study was initiated to develop an efficient and novel antimicrobial approach which combines physical treatments (UV-A or mild heat) and generally recognized as safe lauroyl arginate ethyl (LAE) to inactivate surrogate strains, including Escherichia coli and Listeria innocua. Synergistic inactivation of bacteria resulted in an ∼6-log reduction of target bacteria, while individual treatments resulted in <1.5-log inactivation under the same set of conditions. In addition, the synergistic mechanism between LAE and UV-A/mild heat was evaluated by supplementing with a variety of antioxidants for suppressing oxidative stress and measurement of cell membrane damage by nucleic acid release. These results demonstrate that the synergistic antimicrobial activity of LAE and mild physical stresses was suppressed by supplementation with antioxidants. The research also compared LAE with another membrane-targeting lipopeptide antimicrobial agent, polymyxin B, to understand the uniqueness of LAE-induced synergy. Briefly, differences in modes of action between LAE and polymyxin B were characterized by comparing the MIC, damage to liposomes, and oxidative stress generation. These differences in the mode of action between LAE and polymyxin B suggested that both compounds target cell membrane but significantly differ in mechanisms, including membrane disruption and oxidative stress generation. Overall, this study illustrates synergistic antimicrobial activity of LAE with light or mild heat and indicates a novel oxidative stress pathway that enhances the activity of LAE beyond membrane damage. IMPORTANCE This study highlights an effective antimicrobial processing approach using a novel combination of lauroyl arginate ethyl (LAE) and two different physical treatments, light (UV-A) and mild heat. Both combinations demonstrated synergistic inactivation against a model Gram-negative bacterium or a Gram-positive bacterium or both by a >5-log reduction. Further mechanistic study revealed that oxidative stress is responsible for synergistic inactivation between LAE and UV-A, while both membrane damage and oxidative stress are responsible for the synergistic combination between LAE and mild heat. The mode of action of LAE was further compared to that of polymyxin B and analyzed using artificial membrane model systems and the addition of antioxidants. The proposed combination of LAE and common physical treatments may improve food preservation, food safety, and current sanitation processes for the food industry and the inactivation of pathogenic strains in biomedical environments.

Mechanistic Insight into the Antibacterial Activity of Chitosan Exfoliated MoS2Nanosheets: Membrane Damage, Metabolic Inactivation, and Oxidative Stress

2019 ◽  
Vol 2 (7) ◽  
pp. 2738-2755 ◽  
Author(s):  
Shounak Roy ◽  
Anupam Mondal ◽  
Varnika Yadav ◽  
Ankita Sarkar ◽  
Ruptanu Banerjee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antibacterial Activity ◽  
Membrane Damage ◽  
Mechanistic Insight ◽  
And Oxidative Stress ◽  
Insight Into

scholarly journals Hydrogen peroxide, superoxide, lipid peroxidation and membrane damage in leaves of the tree Prosopis nigra (Fabaceae) under simulated glyphosate drift

2021 ◽  
Vol 13 (1) ◽  
pp. e3170
Author(s):  
Diego Ariel Meloni ◽  
Graciela Inés Bolzón
Keyword(s):  
Oxidative Stress ◽  
Electron Transport Rate ◽  
Stomatal Closure ◽  
Membrane Damage ◽  
Fluorescence Emission ◽  
Modulated Fluorescence ◽  
Glyphosate Drift ◽  
The Impact ◽  
And Oxidative Stress ◽  
Herbicide Glyphosate

Introduction: The tree Prosopis nigra is native to the Western Chaco phytogeographic region, affected by the application of glyphosate in the surrounding crops. Objective: To determine the impact of simulated glyphosate drift on growth, photosynthesis and oxidative stress in P. nigra seedlings. Methods: We simulated drift in seedlings at doses of 0, 200, 400 and 800 g a.e. ha-1 of glyphosate. We also measured gas exchange and modulated fluorescence emission of chlorophyll a. Results: Glyphosate reduced biomass, photosynthetic rate, and stomatal conductance. Doses of 400 and 800 g a.e. ha-1 glyphosate produced photoinhibition. The electron transport rate was sensitive to glyphosate, and it decreased at all doses of the herbicide. Glyphosate generated oxidative stress, and produced damage to membranes, because of the accumulation of H2O2 and O2.. Conclusions: Glyphosate reduces growth and photosynthesis in these seedlings. The inhibition of photosynthesis is due to stomatal closure, and alterations in the photochemical stage, associated with oxidative stress. 

Mitochondrial and lysosomal protective agents ameliorate cytotoxicity and oxidative stress induced by cyclophosphamide and methotrexate in human blood lymphocytes

2019 ◽  
Vol 38 (11) ◽  
pp. 1266-1274 ◽  
Author(s):  
A Salimi ◽  
R Pirhadi ◽  
Z Jamali ◽  
M Ramazani ◽  
BS Yousefsani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Mitochondrial Membrane ◽  
Human Lymphocytes ◽  
Membrane Damage ◽  
Lysosomal Membrane ◽  
Protective Agents ◽  
Blood Lymphocytes ◽  
Ros Formation ◽  
And Oxidative Stress

Cyclophosphamide (CYP) and methotrexate (MTX) have been evaluated for their ability to induce toxicity in human peripheral blood lymphocytes (PBLs) and the protective role of mitochondrial and lysosomal stabilizing agents. The potential toxicity effects of CYP and MTX were measured in vitro by cellular parameters assays such as cellular viability, reactive oxygen species (ROS) formation, mitochondrial membrane permeability transition (mitochondrial membrane potential (MMP)) collapse, lysosomal membrane damage, intracellular reduced glutathione (GSH), extracellular oxidized glutathione (GSSG), and lipid peroxidation. Separately, human lymphocytes were treated with concentrations of 0.1, 0.2, 0.4, 0.8, and 1.6 ng/mL for CYP and 1, 2, 5, and 10 µg/mL for MTX for 6 h. Statistical evaluations showed that CYP and MTX significantly decreased the cell viability at the three highest concentrations when compared with both the negative and solvent controls. In addition, CYP and MTX were significantly induced ROS formation, MMP collapse, lysosomal membrane damage, lipid peroxidation, and GSH depletion compared with the controls. Mitochondrial and lysosomal protective agents like cyclosporine A and chloroquine, respectively, decreased cytotoxicity and oxidative stress induced by CYP and MTX. The present results indicate that CYP and MTX are toxic to human PBLs and their toxicity could be ameliorated by mitochondrial and lysosomal protective agents.

In vitro evaluation of copper oxide nanoparticle-induced cytotoxicity and oxidative stress using human embryonic kidney cells

2019 ◽  
Vol 35 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Anreddy Rama Narsimha Reddy ◽  
Srividya Lonkala
Keyword(s):  
Oxidative Stress ◽  
Cell Membrane ◽  
Membrane Damage ◽  
Human Embryonic Kidney ◽  
Hek 293 ◽  
Cell Membrane Damage ◽  
Cuo Nps ◽  
293 Cells ◽  
And Oxidative Stress

This study aimed to evaluate the in vitro cytotoxicity and oxidative stress induced by the copper oxide nanoparticles (CuO NPs) in human embryonic kidney cell line (HEK-293) cells following exposure. CuO NPs size <50 nm were used in this study. HEK-293 cell cultures were exposed to different concentrations of CuO NPs between 3 µg/ml and 300 µg/ml and quartz (known as cytotoxic agent) and assessed for cell viability-mitochondrial function (MTT assay), cell membrane damage (lactate dehydrogenase (LDH) assay), reduced glutathione (GSH), interleukin-8 (IL-8), and lipid peroxidation levels. The IC50 value of NPs was found to be 65.5 µg/ml. Exposure of HEK cells to CuO NPs (10–300 µg/ml) resulted in concentration-dependent cell membrane damage, increased production of IL-8, increased thiobarbituric acid reactive substance (TBARS), and decreased intracellular GSH levels. The significant increases in IL-8, TBARS, and LDH levels along with decreased GSH levels indicated induction of oxidative stress in cells. Our preliminary data suggest that oxidative stress might contribute to CuO NPs-induced cytotoxicity in HEK-293 cells.

Sign in / Sign up

Export Citation Format

Share Document