Multiple-class antimicrobial resistance surveillance in swine Escherichia coli F4, Pasteurella multocida and Streptococcus suis isolates from Ontario and the impact of the 2004–2006 Porcine Circovirus type-2 Associated Disease outbreak

Swine respiratory disease is associated with productive losses. We evaluated the prevalence of lung lesions with an emphasis on Mycoplasma hyopneumoniae (Mh), porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), as well as the impact on productive parameters in 108 finishing pigs at slaughter. Pathologic, immunohistochemical (IHC) and serologic analyses were performed. Pneumonic processes were observed in 73.1% of the animals. They mainly consisted of cranioventral bronchopneumonia (CBP) (46.3%) and pleuritis (17.6%). Microscopically, bronchointerstitial pneumonia (67.4%) was common and was occasionally combined (27.9%) with interstitial pneumonia (IP). Mh and PCV2-antigens were detected in bronchointerstitial pneumonia (70.7%) and IP cases (33.3%). There were low titers against Mh (18%) and high titers against PRRSV (100%) and PCV2 (65%). Animals with CBP remained at the farm longer; those with >10% of lung parenchyma involvement were sent later (208.8 days old) and had a lower average carcass weight (74.1 kg) and a lower daily weight gain (500.8 gr/day) compared with animals without lesions (567.2 gr/day, 77.7 kg, 200.8 days old). We suggest that animals that do not reach the weight at slaughter should be sent to slaughter regardless to avoid further negative impacts of respiratory disease in productive parameters.

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Production of Escherichia coli-based virus-like particle vaccine against porcine circovirus type 2 challenge in piglets: Structure characterization and protective efficacy validation

Journal of Biotechnology ◽

10.1016/j.jbiotec.2016.02.025 ◽

2016 ◽

Vol 223 ◽

pp. 8-12 ◽

Cited By ~ 13

Author(s):

Xiangfeng Xi ◽

Xiaobing Mo ◽

Yan Xiao ◽

Bo Yin ◽

Chaochao Lv ◽

...

Keyword(s):

Escherichia Coli ◽

Protective Efficacy ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Structure Characterization ◽

Porcine Circovirus ◽

Virus Like Particle

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Association of Porcine Circovirus Type 2 With Vascular Lesions in Porcine Pneumonia

Veterinary Pathology ◽

10.1177/0300985811406888 ◽

2011 ◽

Vol 49 (2) ◽

pp. 264-270 ◽

Cited By ~ 6

Author(s):

L. Szeredi ◽

Á. Dán ◽

N. Solymosi ◽

A. Cságola ◽

T. Tuboly

Keyword(s):

Pasteurella Multocida ◽

Swine Influenza Virus ◽

Clinical Signs ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Vascular Lesions ◽

Porcine Circovirus ◽

Respiratory Syndrome Virus ◽

Respiratory Pathogens

The aim of this study was to evaluate the vasculature in porcine circovirus type 2–infected (PCV2-infected) lungs and to identify the PCV2 subtypes involved in porcine pneumonia. Pulmonary samples from 140 pigs, 2 weeks to 7 months of age, from 36 Hungarian commercial herds with clinical signs of respiratory disease were examined for the presence of respiratory pathogens, with bacterial culture, pathologic evaluation, and immunohistochemistry for PCV2, porcine reproductive respiratory syndrome virus, and swine influenza virus. PCV2 was the most commonly identified pathogen (49 cases) among the 74 of 140 cases (53%) with respiratory pathogens. PCV2 was detected immunohistochemically in the wall of 13% to 100% of pulmonary vessels (mean, 89%) in 38 of 49 cases (78%). Detection of PCV2 antigen was positively correlated with the presence of vascular lesions ( P < .001, odds ratio [OR]: 159.54). Other pathogens capable of vascular injury in swine were found in 29 of 49 of the PCV2-positive cases (59%). The probability of detecting vascular lesions in PCV2-infected lung was higher than in infection with porcine reproductive respiratory syndrome virus ( P < .002, OR: 14.63), Pasteurella multocida infection ( P < .001, OR: 5.75), or Streptococcus spp. infection (not significant, OR: 1.45). Sequence analysis of open reading frame 2 amplicons was possible in 6 PCV2-positive cases, from which 5 cases proved to be PCV2b subtype and 1 case, PCV2a subtype. In conclusion, PCV2 antigen was commonly colocalized with pulmonary vascular lesions in pneumonia in Hungarian swine, and PCV2b was the dominant subtype.

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Efficient expression and purification of porcine circovirus type 2 virus-like particles in Escherichia coli

Journal of Biotechnology ◽

10.1016/j.jbiotec.2016.01.017 ◽

2016 ◽

Vol 220 ◽

pp. 78-85 ◽

Cited By ~ 22

Author(s):

Pei-Ching Wu ◽

Tzu-Yu Chen ◽

Jiun-Ni Chi ◽

Maw-Sheng Chien ◽

Chienjin Huang

Keyword(s):

Escherichia Coli ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Porcine Circovirus ◽

Expression And Purification ◽

Virus Like Particles ◽

Efficient Expression

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Porcine Reproductive and Respiratory Syndrome Virus Infection at the Time of Porcine Circovirus Type 2 Vaccination Has No Impact on Vaccine Efficacy

Clinical and Vaccine Immunology ◽

10.1128/cvi.00338-10 ◽

2010 ◽

Vol 17 (12) ◽

pp. 1940-1945 ◽

Cited By ~ 9

Author(s):

A. Sinha ◽

H. G. Shen ◽

S. Schalk ◽

N. M. Beach ◽

Y. W. Huang ◽

...

Keyword(s):

Vaccine Efficacy ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Porcine Circovirus ◽

Respiratory Syndrome Virus ◽

Lymphoid Tissues ◽

Pcv2 Vaccine ◽

Pcv2 Vaccination ◽

The Impact

ABSTRACT Several porcine circovirus type 2 (PCV2) vaccines are now commercially available and have been shown to be effective at decreasing the occurrence of porcine circovirus-associated disease (PCVAD). Many herds are coinfected with PCV2 and porcine reproductive and respiratory syndrome virus (PRRSV). Some producers and veterinarians are concerned that if pigs are vaccinated for PCV2 at or near the time that they are typically infected with PRRSV, the efficacy of the PCV2 vaccine will be compromised. The impact of PRRSV on PCV2 vaccination is unclear and has not been investigated under controlled conditions. The objective of the present study was to determine whether the presence of PRRSV viremia has an effect on the efficacy of commercial PCV2 vaccinations. Three-week-old PCV2-negative conventional pigs with passively derived anti-PCV2 antibodies were either vaccinated with one of three commercial PCV2 vaccines or left nonvaccinated. A portion of the pigs were infected with PRRSV 1 week prior to PCV2 vaccination. To determine vaccine efficacy, a PCV2 challenge was conducted at 8 weeks of age. PCV2 vaccination, regardless of PRRSV infection status at the time of vaccination, was similarly effective in inducing an anti-PCV2 IgG response in the presence of maternally derived immunity and in protecting the pigs from PCV2 challenge, as determined by a reduction in the level of PCV2 viremia and a reduction in the prevalence and amount of PCV2 antigen in lymphoid tissues in vaccinated pigs compared to nonvaccinated pigs. The results indicate that acute PRRSV infection at the time of PCV2 vaccination has no adverse effect on PCV2 vaccine efficacy.

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Vaccination of Sows against Porcine Circovirus Type 2 (PCV2) in a Subclinically Infected Herd Does Not Impact Reproductive Performance

Agriculture ◽

10.3390/agriculture10120639 ◽

2020 ◽

Vol 10 (12) ◽

pp. 639

Author(s):

Piotr Cybulski ◽

Aleksandra Woźniak ◽

Katarzyna Podgórska ◽

Tomasz Stadejek

Keyword(s):

Reproductive Performance ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Porcine Circovirus ◽

Infected Herd ◽

Swine Production ◽

Control Programs ◽

Pcv2 Vaccination ◽

The Impact

Porcine circovirus type 2 (PCV2) vaccination in piglets has become one of the crucial and indisputable procedures in modern swine production. The role of PCV2 vaccination in breeding animals is less explored. In the present study, the evaluation of the impact of sow vaccination on reproductive parameters was performed in a high health status, PCV2 subclinically infected herd of 3200 sows. The comparison of the number of liveborn, stillborn, and weak born piglets between groups of sows vaccinated on 1 or 28 days after weaning, or nonvaccinated, did not show any statistically significant differences. Although in the tested farm the vaccination of sows appeared to have no effect on reproductive performance, the results should not be generalized. Careful and individualized analysis of diagnostic and production data is crucial in economizing infectious disease control programs in sow herds and pig populations.

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