Expression profiles for 14-3-3 zeta and CCL20 in pancreatic cancer and chronic pancreatitis

21150 Background: The aim of the study was to analyze the gene expression profile of pancreatic cancer by multivariate methods of class prediction. Methods: The snap-frozen or RNA-later preserved samples of 18 pancreatic adenocarcinomas, 9 chronic pancreatitis cases and 6 specimens collected from microscopically unchanged pancreas (N/CP) were analyzed by HG-U133 Plus 2.0 oligonucleotide microarrays (Affymetrix). The obtained dataset was pre-processed using GC-RMA method, gene selection was carried out both by class comparison methods (Welch test with Benjamini-Hochberg correction, False Discovery Rate FDR<5%) and by our own algorithms of class prediction, based on Support Vector Machines technique (Recurrent Feature Replacement and Bootstrap-Based Feature Ranking). Real- time quantitative PCR (Q-PCR) was carried out on Applied Biosystems 7900 HT machine, with Universal Probe Library (Roche) fluorescent probes and normalization by three reference genes index (geNorm, Vandesompele et al.). Results: We compared gene expression profiles between pancreatic cancer samples and N/CP specimens. 23850 probesets significantly differentiated between these three classes (FDR<5%). No ideal discrimination between cancer and N/CP samples was possible by any of single markers. We selected the optimal multi- gene classifier by Support Vector Machines, using Bootstrap-Based Feature Ranking method. The smallest classifier resulting in 100% accuracy consisted of three genes, 45 genes were included in more than half of the diagnostic genesets obtained during bootstrapping process. 14 genes were selected for Q-PCR validation, again none of them ideally discriminated between cancer and normal specimens, with the area under the receiver-operating-characteristic curve ranging from 0.82–0.93. Three-gene combinations allowed for proper classification of all samples. Conclusions: The multi-gene classifier, derived both by microarray technique and Q-PCR analysis, is properly discriminating between pancreatic cancer and chronic pancreatitis/normal pancreas. At least three genes must be included in the classifier to obtain satisfying accuracy. MO and MJ equally contributed to the study. No significant financial relationships to disclose.

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EUS-GUIDED TISSUE ACQUISITION IN CHRONIC PANCREATITIS: DIFFERENTIAL DIAGNOSIS BETWEEN PANCREATIC CANCER AND PSEUDOTUMORAL MASSES USING EUS-FINE NEEDLE ASPIRATION OR CORE BIOPSY

10.1055/s-0040-1704279 ◽

2020 ◽

Author(s):

R Grassia ◽

N Imperatore ◽

P Capone ◽

F Cereatti ◽

E Forti ◽

...

Keyword(s):

Pancreatic Cancer ◽

Differential Diagnosis ◽

Chronic Pancreatitis ◽

Fine Needle Aspiration ◽

Core Biopsy ◽

Needle Aspiration ◽

Fine Needle ◽

Tissue Acquisition

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Faculty Opinions recommendation of Surgery for chronic pancreatitis decreases the risk for pancreatic cancer: a multicenter retrospective analysis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717960350.793463331 ◽

2012 ◽

Author(s):

Zhao-Shen Li ◽

Zhuan Liao

Keyword(s):

Pancreatic Cancer ◽

Chronic Pancreatitis ◽

Retrospective Analysis

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RESULTS OF SURGICAL TREATMENT OF PATIENTS WITH CHRONIC PANCREATITIS AND THE RISK OF CANCER AFTER SURGICAL TREATMENT

Problems in oncology ◽

10.37469/0507-3758-2018-64-2-228-233 ◽

2018 ◽

Vol 64 (2) ◽

pp. 228-233

Author(s):

Vladimir Lubyanskiy ◽

Vasiliy Seroshtanov ◽

Ye. Semenova

Keyword(s):

Pancreatic Cancer ◽

Chronic Pancreatitis ◽

Surgical Treatment ◽

Fibrous Tissue ◽

Pancreatic Head ◽

Head Region ◽

Intestinal Anastomoses ◽

Resection Of The Pancreas ◽

Risk Of Cancer

The aim: To analyze results of surgical treatment of patients with chronic pancreatitis (CP) and to assess the causes of pancreatic cancer after surgical treatment. Materials and methods: 137 patients had duodenum-preserving resections of the pancreas. Results: In the histological examination of the pancreas it was established that the growth of fibrous tissue was registered in patients with CP., which in 19 (13.8%) almost completely replaced the acinar tissue. In the long term after the operation from 6 months to 2 years in 8 patients (5.8%) pancreatic cancer was detected. Possible causes of tumor origin were analyzed, the value of preservation of ductal hypertension, which affects the state of the duct’s epithelium, was established. The most commonly used for treatment of chronic pancreatitis the Frey surgery removed pancreatic hypertension but in two patients during the operation an insufficient volume of the pancreatic head was reconstructed. In the case of the abandonment of a large array of fibrous tissue, local hypertension was retained in the region of the ductal structures of the head, which led to the transformation of the duct epithelium. An essential factor in the problem of the preservation of pancreatic hypertension were the stenosis of pancreatic intestinal anastomoses, they arose in the long term in 4 operated patients. With stenosis of anastomosis after duodenum-preserving resection both the hypertension factor and the regeneration factor could be realized, which under certain circumstances might be significant. Conclusion: After resection of the pancreas for CP cancer was diagnosed in 5.8% of patients. The main method of preventing the risk of cancer was performing the Frey surgery for CP eliminating pancreatic hypertension in the head region of the pancreas. Diagnosis of stenosis in the late period after resection of the pancreas was an important element in the prevention of recurrence of cancer since a timely reconstructive operation could improve the drainage of duct structures.

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Differences in the Expression of Glutathione S-Transferases in Normal Pancreas, Chronic Pancreatitis, Secondary Chronic Pancreatitis, and Pancreatic Cancer

Pancreas ◽

10.1097/00006676-200204000-00013 ◽

2002 ◽

Vol 24 (3) ◽

pp. 291-297 ◽

Cited By ~ 12

Author(s):

Alexis B. Ulrich ◽

Bruno M. Schmied ◽

Jens Standop ◽

Matthias B. Schneider ◽

Terence A. Lawson ◽

...

Keyword(s):

Pancreatic Cancer ◽

Chronic Pancreatitis ◽

Normal Pancreas ◽

Glutathione S Transferases

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A Circulating Exosome RNA Signature Is a Potential Diagnostic Marker for Pancreatic Cancer, a Systematic Study

Cancers ◽

10.3390/cancers13112565 ◽

2021 ◽

Vol 13 (11) ◽

pp. 2565

Author(s):

Yixing Wu ◽

Hongmei Zeng ◽

Qing Yu ◽

Huatian Huang ◽

Beatrice Fervers ◽

...

Keyword(s):

Pancreatic Cancer ◽

Chronic Pancreatitis ◽

Early Detection ◽

Validation Dataset ◽

Healthy Controls ◽

Rna Seq ◽

Healthy Individuals ◽

Kras Mutations ◽

Significant Difference ◽

Rna Signature

Several exosome proteins, miRNAs and KRAS mutations have been investigated in the hope of carrying out the early detection of pancreatic cancer with high sensitivity and specificity, but they have proven to be insufficient. Exosome RNAs, however, have not been extensively evaluated in the diagnosis of pancreatic cancer. The purpose of this study was to investigate the potential of circulating exosome RNAs in pancreatic cancer detection. By retrieving RNA-seq data from publicly accessed databases, differential expression and random-effects meta-analyses were performed. The results showed that pancreatic cancer had a distinct circulating exosome RNA signature in healthy individuals, and that the top 10 candidate exosome RNAs could distinguish patients from healthy individuals with an area under the curve (AUC) of 1.0. Three (HIST2H2AA3, LUZP6 and HLA-DRA) of the 10 genes in exosomes had similar differential patterns to those in tumor tissues based on RNA-seq data. In the validation dataset, the levels of these three genes in exosomes displayed good performance in distinguishing cancer from both chronic pancreatitis (AUC = 0.815) and healthy controls (AUC = 0.8558), whereas a slight difference existed between chronic pancreatitis and healthy controls (AUC = 0.586). Of the three genes, the level of HIST2H2AA3 was positively associated with KRAS status. However, there was no significant difference in the levels of the three genes across the disease stages (stages I–IV). These findings indicate that circulating exosome RNAs have a potential early detection value in pancreatic cancer, and that a distinct exosome RNA signature exists in distinguishing pancreatic cancer from healthy individuals.

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