scholarly journals Multiple PCR assay based on the cigR gene for detection of Salmonella spp. and Salmonella Pullorum/Gallinarum identification

2020 ◽  
Vol 99 (11) ◽  
pp. 5991-5998
Author(s):  
Ying-ying Zhou ◽  
Xi-long Kang ◽  
Chuang Meng ◽  
Dan Xiong ◽  
Ying Xu ◽  
...  
Keyword(s):  
Pcr Assay ◽  
Salmonella Spp ◽  
Salmonella Pullorum ◽  
Multiple Pcr

scholarly journals Occurrence, antimicrobial susceptibility patterns and genotypic relatedness of Salmonella spp. isolates from captive wildlife, their caretakers, feed and water in India

2018 ◽  
Vol 146 (12) ◽  
pp. 1543-1549
Author(s):  
Arockiasamy Arun Prince Milton ◽  
Rajesh Kumar Agarwal ◽  
Govindarajan Bhuvana Priya ◽  
Cheruplackal Karunakaran Athira ◽  
Mani Saminathan ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Animal Species ◽  
Multidrug Resistant ◽  
Wild Animal ◽  
Diffusion Method ◽  
Pcr Assay ◽  
Direct Pcr ◽  
Salmonella Spp ◽  
Culture Methods ◽  
Faecal Samples

AbstractOccurrence of Salmonella spp. in captive wild animal species in India is largely unknown. The purpose of this study was to determine the occurrence of different Salmonella serotypes, antimicrobial resistance patterns and genotypic relatedness of recovered isolates. A total of 370 samples including faecal (n = 314), feed and water (n = 26) and caretakers stool swabs (n = 30) were collected from 40 different wild animal species in captivity, their caretakers, feed and water in four zoological gardens and wildlife enclosures in India. Salmonellae were isolated using conventional culture methods and tested for antimicrobial susceptibility with the Kirby–Bauer disc diffusion method. Salmonella isolates were serotyped and genotyping was performed using enterobacterial repetitive intergenic consensus (ERIC) PCR and 16S rRNA sequencing. Animal faecal samples were also subjected to direct PCR assay. Salmonella was detected in 10 of 314 (3.1%) faecal samples by isolation and 18 of 314 (5.7%) samples by direct PCR assay; one of 26 (3.8%) feed and water samples and five of 30 (16.7%) caretakers stool swabs by isolation. Salmonella was more commonly isolated in faecal samples from golden pheasants (25%; 2/8) and leopard (10%; 2/20). Salmonella enterica serotypes of known public health significance including S. Typhimurium (37.5%; 6/14), S. Kentucky (28.5%; 4/14) and S. Enteritidis (14.3%; 2/14) were identified. While the majority of the Salmonella isolates were pan-susceptible to the commonly used antibiotics. Seven (43.7%; 7/16) of the isolates were resistant to at least one antibiotic and one isolate each among them exhibited penta and tetra multidrug-resistant types. Three S. Kentucky serotype were identified in a same golden pheasants cage, two from the birds and one from the feed. This serotype was also isolated from its caretaker. Similarly, one isolate each of S. Typhimurium were recovered from ostrich and its caretaker. These isolates were found to be clonally related suggesting that wildlife may serve as reservoir for infections to humans and vice versa. These results emphasise the transmission of Salmonella among hosts via environmental contamination of feces to workers, visitors and other wildlife.

Application of an optimized 18-h method involving one step culturing and single primer-based PCR assay for detection of Salmonella spp. in foods

Food Control ◽  
2010 ◽  
Vol 21 (5) ◽  
pp. 593-598 ◽  
Author(s):  
Sudsai Trevanich ◽  
Sujeeporn Tiyapongpattana ◽  
Takahisa Miyamoto
Keyword(s):  
Pcr Assay ◽  
Salmonella Spp ◽  
One Step ◽  
Single Primer

Rapid Detection for Salmonella spp. by Ultrafast Real-time PCR Assay

2018 ◽  
Vol 33 (1) ◽  
pp. 50-57
Author(s):  
Seok Hwan Kim ◽  
Yu-Si Lee ◽  
In-Sun Joo ◽  
Hyo Sun Kwak ◽  
Gyung Tae Chung ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Salmonella Spp

Development of a quantitative real-time PCR assay for viable Salmonella spp. without enrichment

Food Control ◽  
2015 ◽  
Vol 57 ◽  
pp. 185-189 ◽  
Author(s):  
Lili Xiao ◽  
Zhaohuan Zhang ◽  
Xiaohong Sun ◽  
Yingjie Pan ◽  
Yong Zhao
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Salmonella Spp

A New Real-Time PCR Assay for the Specific Detection of Salmonella spp. Targeting the bipA Gene

2008 ◽  
Vol 1 (4) ◽  
pp. 236-242 ◽  
Author(s):  
Laia Calvó ◽  
Asunción Martínez-Planells ◽  
Joana Pardos-Bosch ◽  
L. Jesús Garcia-Gil
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Pcr Assay ◽  
Salmonella Spp

Screening of Salmonella enterica Serovars, Typhi, Typhimurium, and Enteritidis in Raw Milk and Dairy Products in South-Khorasan, Iran: Conventional versus Molecular Method

2020 ◽  
Vol 16 (5) ◽  
pp. 763-767
Author(s):  
Tayebeh Zeinali ◽  
Kobra Naseri ◽  
Nasrin Zandi ◽  
Matin Khosravi
Keyword(s):  
Salmonella Enterica ◽  
Dairy Products ◽  
Culture Method ◽  
Raw Milk ◽  
Positive Sample ◽  
Contamination Rate ◽  
Pcr Assay ◽  
Salmonella Spp ◽  
South Khorasan ◽  
Milk And Dairy Products

Background and Objective: Food-borne Salmonellosis has been reported as the second most common bacterial infection. Salmonella enterica subsp. enterica serotype enteritidis (S. Enteritidis) and S. enterica subsp. enterica serotype typhimurium (S. Typhimurium) are the most common serotypes worldwide as salmonellosis agents. Salmonella yyphi is the causative agent of typhoid fever worldwide. The purpose of the present study was to determine the contamination rate of raw milk and dairy products to Salmonella typhi, S. typhimurium and S. Enteritidis in South-Khorasan, Iran. It is very important in food safety risk assessment and human health. Methods: A total of 260 raw milk and 181 dairy products were obtained from South-Khorasan, Iran. Dairy samples were pre-enriched in buffered peptone water and enriched in Rappaport Vassiliadis (RV). Raw milk was enriched in RV. Plating of the enriched samples was carried out on Xylose Lysine Desoxycholate (XLD) agar and Brilliant Green agar (BGA). All of the enriched samples were also tested by M-PCR for detection of S. typhi, S. typhimurium and S. Enteritidis. Results: Among the 441 tested samples only 4 samples were contaminated with Salmonella spp. in culture method. PCR assay, didn’t find any positive sample regarding Salmonella spp. In chi-square test, the difference of two methods of isolation was significant (P< 0.05). Conclusions: In conclusion, the results of the present study showed a good hygienic state of raw milk and dairy products. Enrichment based PCR assay is more economical than time-consuming culture method for Salmonella detection.

scholarly journals A comparative survey between non-systemic Salmonella spp. (paratyphoid group) and systemic Salmonella Pullorum and S. Gallinarum with a focus on virulence genes

2017 ◽  
Vol 37 (10) ◽  
pp. 1064-1068 ◽  
Author(s):  
Claudete S. Astolfi-Ferreira ◽  
Marcelo R.S. Pequini ◽  
Luis F.N. Nuñez ◽  
Silvana H. Santander Parra ◽  
Ruy Chacon ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Virulence Gene ◽  
Gene Profile ◽  
Salmonella Spp ◽  
Salmonella Pullorum ◽  
Comparative Survey ◽  
Virulence Gene Profile

ABSTRACT: A comparative survey between non-systemic (paratyphoid Salmonellae) and systemic (S. Pullorum and S. Gallinarum) Salmonella strains was performed to produce a virulence gene profile for differentiation among the groups. The following virulence genes were evaluated: invA, spvC, sefC, pefA, fimY, sopB, sopE1, stn and avrA. There are substantial differences among paratyphoid Salmonellae, S. Pullorum, and S. Gallinarum regarding the genes sefC, spvC, sopE1 and avrA. A higher frequency of sefC, spvC, sopE1 and avrA genes were detected in S. Gallinarum and S. Pullorum when compared with strains from the paratyphoid group of Salmonella. These results may be useful for differentiating among different groups and serotypes.

scholarly journals Validation of a Modified Version of Actero™ Salmonella Enrichment Media for Rapid Detection of Salmonella spp. in Environmental and Food Samples

2020 ◽  
Vol 103 (5) ◽  
pp. 1326-1337
Author(s):  
Afia Boumail ◽  
Alex Eyraud ◽  
Mounia Akassou ◽  
Mélanie Geffroy ◽  
Jean-Félix Sicard ◽  
...  
Keyword(s):  
Food Samples ◽  
Culture Broth ◽  
Probability Of Detection ◽  
Alternative Methods ◽  
Pet Food ◽  
Pcr Assay ◽  
Salmonella Spp ◽  
Environmental Surfaces ◽  
Chicken Carcass ◽  
Detection Assay

Abstract Background Actero™ Salmonella Enrichment Media1 (Actero™ Salmonella) is a culture broth developed to recover Salmonella spp. from foods and environmental surfaces. Performance of Actero™ Salmonella broth has already been assessed and validated (AOAC Performance Tested MethodSM 041403) for the detection of Salmonella spp. in various foods, feeds and environmental samples. Objective This study aimed to validate the performance of a modified version of Actero™ Salmonella broth by incorporating one of the two liquid supplements into the powdered formula. Methods Inclusivity, exclusivity, stability, and lot-to-lot studies were carried out. Raw ground beef, chicken carcass rinse, dry pet food and stainless steel samples were enriched for 14–20 h at 35–39°C and analyzed using real-time PCR assay as well as by direct plating. Results The Probability of Detection assay confirmed the equivalent performance of the alternative methods as compared to the reference methods. All Salmonella strains, except Salmonella II : 57: z29:-, were able to grow in Actero™ Salmonella broth. One-half of the non-target strains did not grow in Actero™ Salmonella broth, whereas the atypical for Salmonella growth was observed for other non-target microorganisms subsequently plated onto selective and differential agars. Lot-to-lot consistency was demonstrated for three consecutively manufactured lots of the broth. The liquid broth was proven to be stable at 4°C for up to 9 weeks of storage. Conclusions and Highlights The incorporation of one of the two specific supplements into a powdered formula of Actero™ Salmonella broth made it more convenient to use without compromising the performance and accuracy.

Efficiency of Electrolyzed Oxidizing Water for Inactivation of Salmonella spp. and Inoculated Shell Eggs

2009 ◽  
Vol 5 (3) ◽  
Author(s):  
Qijun Ma ◽  
Baoming Li ◽  
Chaoyuan Wang ◽  
Yingying Ji ◽  
Shuhua Wang ◽  
...  
Keyword(s):  
Bactericidal Activity ◽  
Salmonella Enteritidis ◽  
Ph Value ◽  
Treatment Results ◽  
Salmonella Spp ◽  
Shell Eggs ◽  
Pure Cultures ◽  
Salmonella Pullorum ◽  
Alkaline Electrolyzed Water ◽  
Bactericidal Efficiency

The risk of a salmonellosis outbreak from consuming contaminated eggs is a societal and governmental concern. The bactericidal efficiency of electrolyzed oxidizing (EO) water for inactivation of salmonella and artificially inoculated shell eggs were evaluated in this study. The effects of available chlorine concentration (ACC, 0.1-2.0 mg/l) and pH value (2.3, 3.5, 4.6, 5.7 and 6.5) on the bactericidal activity of EO water were investigated by using pure cultures (7.0-8.0 log10 CFU/ml) of Salmonella pullorum and Salmonella enteritidis strains. EO water was effective for inactivation of both pathogens. The bactericidal activity increased with increasing the ACC of EO water. The bacteria were completely inactivated when the ACC of EO water was higher than 1.0 mg/l. At a sufficient ACC (greater than 3.0 mg/l), the bactericidal activity of EO water was independent of its pH value. A 100% inactivation (reduction of 6.7 log10 CFU/g) of both pathogens on the surface of shell eggs was achieved at an ACC of 20 mg/l except for the treatment of EO water spraying. Shell eggs soaked in alkaline electrolyzed water followed by soaked in EO water was more effective to reduce the viable counts of both pathogens compared to EO water used alone. No survival of both pathogens was detected in EO water after washing treatment. Results indicated that EO water is a promising and powerful disinfectant agent for shell eggs washing processing.

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