scholarly journals Multicenter Evaluation of the BD Max Enteric Bacterial Panel PCR Assay for Rapid Detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga Toxin 1 and 2 Genes

2015 ◽  
Vol 53 (5) ◽  
pp. 1639-1647 ◽  
Author(s):  
S. M. Harrington ◽  
B. W. Buchan ◽  
C. Doern ◽  
R. Fader ◽  
M. J. Ferraro ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Campylobacter Spp

Microbiological Quality of Retail Imported Unprepared Whole Lettuces: A PHLS Food Working Group Study

1999 ◽  
Vol 62 (4) ◽  
pp. 325-328 ◽  
Author(s):  
C. LITTLE ◽  
D. ROBERTS ◽  
E. YOUNGS ◽  
J. de LOUVOIS
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Microbiological Quality ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Natural Microflora ◽  
Production Practices ◽  
Campylobacter Spp

A study of imported unprepared whole lettuces sampled from supermarkets, greengrocers, shops, and market stalls found that all were of acceptable microbiological quality. Twenty-seven out of 151 (18%) imported lettuce samples had Enterobacteriaceae levels of 104 CFU/g or more. However, these bacteria that constitute part of the natural microflora of unprepared vegetables may also be derived from the soil and/or by poor handling. The pathogens, Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli O157:H7, Vibrio cholerae, Listeria monocytogenes, and also Escherichia coli, an indicator of fecal contamination, were not detected in any imported lettuces, indicating that hygiene, harvesting, and production practices were good. Imported lettuces with Enterobacteriaceae levels of 104 CFU/g or more varied with type of retail premises and the temperature at which the lettuces were displayed. Samples from greengrocers, shops, and market stalls were more likely to contain Enterobacteriaceae at levels in excess of 104 CFU/g than those from supermarkets.

scholarly journals Norovirus Is the Most Frequent Cause of Diarrhea in Hospitalized Patients in Monterrey, Mexico

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 672
Author(s):  
Néstor Casillas-Vega ◽  
Fernanda Flores-Rodríguez ◽  
Israel Sotelo-Coronado ◽  
Magda Elizabeth Vera-García ◽  
Aldo García-Heredia ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Microscopic Observation ◽  
Hospitalized Patients ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Clostridioides Difficile ◽  
Shigella Spp ◽  
Level Hospital ◽  
Clinical Problems ◽  
Campylobacter Spp

Little information is available regarding the pathogens that cause diarrhea in hospitalized patients who also have various clinical problems. The purpose of this study was to determine the presence of pathogens in fecal samples of hospitalized patients all suffering diarrhea in addition to other problems in Mexico. Diarrheic stools from 240 patients were obtained in a third-level hospital in Monterrey, Mexico. PCR was used for the detection of Salmonella spp., Shigella spp., Campylobacter spp., Yersinia spp., Aeromonas spp., Clostridioides difficile, and norovirus GI and GII. The presence of trophozoites, cysts of protozoa, eggs, and/or helminth larvae was determined by microscopic observation. Of the 240 patients analyzed, 40.4% presented at least one of the pathogens analyzed. Norovirus was the pathogen most frequently found (28.6%), followed by bacteria (11.7%), and parasites (8.3%). The majority of co-infections were parasites + norovirus, and bacteria + norovirus. Norovirus was detected mainly in children aged 0 to 10 years (9/15, 60%). Patients aged 0–20 years did not present co-infections. Entamoeba coli and Entamoeba histolytica were the most common parasites, (8/240), and Salmonella was the most prevalent bacteria (10/240). This information can help design specific strategies useful for hospitalized people with a compromised status.

Rapid Detection for Salmonella spp. by Ultrafast Real-time PCR Assay

2018 ◽  
Vol 33 (1) ◽  
pp. 50-57
Author(s):  
Seok Hwan Kim ◽  
Yu-Si Lee ◽  
In-Sun Joo ◽  
Hyo Sun Kwak ◽  
Gyung Tae Chung ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Salmonella Spp

Multiplex PCR method for the detection of human norovirus, Salmonella spp., Shigella spp., and shiga toxin producing Escherichia coli in blackberry, coriander, lettuce and strawberry

2021 ◽  
pp. 103926
Author(s):  
Omar Hernández Hernández ◽  
Ana L. Gutiérrez-Escolano ◽  
Cleo Cancio-Lonches ◽  
Montserrat H. Iturriaga ◽  
Juan Ramiro Pacheco-Aguilar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Shiga Toxin ◽  
Salmonella Spp ◽  
Human Norovirus ◽  
Shigella Spp ◽  
Pcr Method

scholarly journals Prevalence and Antimicrobial Resistance of Enteropathogenic Bacteria in Yellow-Legged Gulls (Larus michahellis) in Southern Italy

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 275
Author(s):  
Tamara Pasqualina Russo ◽  
Antonino Pace ◽  
Lorena Varriale ◽  
Luca Borrelli ◽  
Antonio Gargiulo ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Enteropathogenic Bacteria ◽  
Enteric Diseases ◽  
Large Populations ◽  
Larus Michahellis ◽  
Resistant Strains ◽  
Campylobacter Spp

Wild birds may host and spread pathogens, integrating the epidemiology of infectious diseases. Particularly, Larus spp. have been described as responsible for the spread of many enteric diseases, primarily because of their large populations at landfill sites. The aim of this study was to examine the role of yellow-legged gulls as a source of enteropathogenic bacteria such as Campylobacter spp., Salmonella spp., Shiga toxin-producing Escherichia coli and Yersinia spp., with particular attention to antibiotic-resistant strains. Enteropathogenic bacteria were isolated from 93/225 yellow-legged gulls examined from April to July, during a four-year period (2016–2019). Specifically, Campylobacter spp. was isolated from 60/225 samples (26.7%), and identified as C. coli (36/60) and as C. jejuni (24/60). Salmonella spp. was isolated from 3/225 samples (1.3%), and identified as Salmonella arizonae. Shiga toxin-producing E. coli were isolated from 30/225 samples (13.3%) samples, and serotyped as E. coli O128 (12/30) O26 (9/30), O157 (6/30) and O11 (3/30); Yersinia spp. was never detected. Isolated strains exhibited multidrug resistance, including vitally important antibiotics for human medicine (i.e., fluoroquinolones, tetracyclines). Our study emphasizes the importance of yellow-legged gulls as potential reservoirs of pathogenic and resistant strains and their involvement in the dissemination of these bacteria across different environments, with resulting public health concerns.

A Robust and Poisson Validated Quantitative 5′ Nuclease TaqMan® Real-Time PCR Assay Targeting fimA for the Rapid Detection of Salmonella spp. in Food

2012 ◽  
Vol 6 (4) ◽  
pp. 991-995 ◽  
Author(s):  
E. Mann ◽  
I. Hein ◽  
P. Mester ◽  
B. Stessl ◽  
P. Rossmanith ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Salmonella Spp

scholarly journals Contamination Status of Salmonella spp., Shigella spp. and Campylobacter spp. in Surface and Groundwater of the Kelani River Basin, Sri Lanka

Water ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2187
Author(s):  
M.G.Y.L. Mahagamage ◽  
M.V.S.C. Pathirage ◽  
Pathmalal M. Manage
Keyword(s):  
Surface Water ◽  
Sri Lanka ◽  
River Basin ◽  
Total Coliform ◽  
Salmonella Spp ◽  
E Coli ◽  
Sampling Locations ◽  
Shigella Spp ◽  
Kelani River ◽  
Campylobacter Spp

Waterborne diseases are a global problem that causes more than 2.2 million deaths annually. Therefore, the present study was focused on microbiological contamination of both ground and surface water by means of total coliform, Escherichia coli (E. coli), Salmonella spp., Shigella spp. and Campylobacter spp. Seventy two groundwater and 45 surface water sampling locations were selected to collect water from the head, transitional and meandering regions of the Kelani River Basin for a period of one year (both dry and wet seasons). The results of the study revealed that the entire Kelani River basin was contaminated with total coliform and E. coli bacteria and almost all the sampling locations exceed Sri Lanka Standards Institute (SLSI) guideline value given for drinking water (0 CFU/100 mL). Further, in groundwater, 17 locations were positive for Salmonella spp., whereas only 2 locations were positive for Campylobacter spp. In surface water, 26 and three sampling locations were positive for Salmonella spp. and Campylobacter spp., respectively. In this study, 23 different human pathogenic serovars were isolated and the Salmonella enterica serovar Kentucky was identified as the commonest type. Thus, the result of the study revealed that the consumption of raw water from the Kelani River Basin is unsafe and possible to cause gastrointestinal diseases.

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