Oral HPV prevalence assessment by Linear Array vs. SPF10 PCR-DEIA-LiPA25 system in the HPV Infection in Men (HIM) study

A high-risk population, formed by 79 consecutive male attendees of our sexually transmissible infections clinic, and who did not have any overt signs of human papillomavirus (HPV) infection, were examined. Oral HPV prevalence (37%) was much higher than previously reported. Periurethral HPV-DNA was found in 49% of the patients and anal HPV-DNA was found in 43% of the patients. Considering the high prevalence of oral HPV obtained in the current study, it is important to further investigate the burden of oral HPV, not only in men who have sex with men, but also in heterosexual men and in women.

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HPV Infection in a Cohort of HIV-Positive Men and Women: Prevalence of Oncogenic Genotypes and Predictors of Mucosal Damage at Genital and Oral Sites

Journal of Sexually Transmitted Diseases ◽

10.1155/2013/915169 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8

Author(s):

Giulia Marchetti ◽

Laura Comi ◽

Teresa Bini ◽

Marco Rovati ◽

Francesca Bai ◽

...

Keyword(s):

Mucosal Damage ◽

Hpv Infection ◽

Cross Sectional Study ◽

Hiv Positive ◽

Low Grade ◽

Abnormal Cytology ◽

Cross Sectional ◽

Squamous Cells ◽

Hpv Prevalence ◽

Oral Hpv

The aim of this study was to assess the prevalence of HPV infection and determinants of abnormal cytology in HIV-positive patients. In a cross-sectional study, patients of both sexes, asymptomatic for HPV, underwent anorectal (men)/cervical (women) and oral swabs. Cytology and HPV-PCR detection/genotyping (high- and low-risk genotypes, HR-LR/HPV) were performed. A total of 20% of the 277 enrolled patients showed oral HPV, with no atypical cytology; in men, anal HPV prevalence was 81% with 64% HR genotypes. In women, cervical HPV prevalence was 58% with 37% HR-HPV. The most frequent genotypes were HPV-16 and HPV-18; 37% of men and 20% of women harbored multiple genotypes. Also, 47% of men showed anal squamous intraepithelial lesions (SILs); 6% had high- and 35% low-grade SILs (HSILs/LSILs); 5% had atypical squamous cells of undetermined significance (ASC-US). HR-HPV was independently associated with anal-SIL in men (P=0.039). Moreover, 37% of women showed cervical SIL: 14 ASC-US, 15 LSILs, 4 HSILs, and 1 in situ cancer. The presence of both LR and HR-HPV in women was independently associated with SIL (P=0.003 and P=0.0001). HR-HPV and atypical cytology were frequently identified in our cohort. HPV screening should be mandatory in HIV-infected subjects, and vaccine programs for HPV-negative patients should be implemented.

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Differences in Factors Associated With High- and Low-Risk Oral Human Papillomavirus Genotypes in Men

The Journal of Infectious Diseases ◽

10.1093/infdis/jiaa693 ◽

2020 ◽

Author(s):

Deepti Bettampadi ◽

Brittney Dickey ◽

Martha Abrahamsen ◽

Bradley Sirak ◽

Maria Luiza Baggio ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Sexual Behaviors ◽

Hpv Infection ◽

Low Risk ◽

Factors Associated ◽

Hpv Prevalence ◽

Oral Hpv ◽

Multivariable Models ◽

High Risk Hpv

Abstract Background Human papillomavirus (HPV) causes oral warts and oropharyngeal cancer (OPC). Human papillomavirus-attributable OPC incidence among men is significantly increasing worldwide, yet few studies have reported oral HPV across multiple countries or examined factors associated with low- and high-risk HPV separately. Methods Oral gargles from 3095 men in the multinational HPV Infection in Men (HIM) Study were HPV genotyped. Multivariable models assessed factors independently associated with high-risk and low-risk HPV prevalence. Results The prevalence of high-risk and low-risk HPV was 6.0% and 2.8%, respectively. Greater number of sexual partners was only associated with high-risk HPV (1.88; 95% confidence interval [CI], 1.22–2.90) prevalence. In multivariable models, residing in Mexico (1.66; 95% CI, 1.15–2.40) and smoking (1.66; 95% CI, 1.13–2.44) were significantly associated with high-risk HPV, and history of consistent gum bleeding (2.16; 95% CI, 1.35–3.45) was significantly associated with low-risk HPV. Gender of the sexual partner did not alter the results for either high- or low-risk HPV endpoints. Conclusions Different factors were independently associated with high- and low-risk oral HPV. Oral sexual behaviors were associated with high-risk HPV, and oral health was associated with low-risk HPV. High-risk HPV prevalence differed by country of residence, highlighting the need for additional studies in multiple countries.

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Oral HPV prevalence in women positive for cervical HPV infection and their sexual partners: a German screening study

European Archives of Oto-Rhino-Laryngology ◽

10.1007/s00405-016-3953-1 ◽

2016 ◽

Vol 273 (7) ◽

pp. 1933-1942 ◽

Cited By ~ 7

Author(s):

Ralf B. Uken ◽

Oliver Brummer ◽

Carolin von Schubert-Bayer ◽

Thomas Brodegger ◽

Ingo U. Teudt

Keyword(s):

Hpv Infection ◽

Sexual Partners ◽

Hpv Prevalence ◽

Screening Study ◽

Oral Hpv

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Predictors of Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in HIV-Infected and Uninfected Men Who Have Sex with Men of the OHMAR Study

Journal of Clinical Medicine ◽

10.3390/jcm10132804 ◽

2021 ◽

Vol 10 (13) ◽

pp. 2804

Author(s):

Massimo Giuliani ◽

Tarik Gheit ◽

Francesca Rollo ◽

Massimo Tommasino ◽

Alessandra Latini ◽

...

Keyword(s):

Linear Array ◽

Hpv Infection ◽

Oral Infection ◽

Human Papillomaviruses ◽

Oral Sex ◽

Sex Partners ◽

Oral Rinse ◽

Luminex Technology ◽

Oral Hpv ◽

Sex With Men

Mucosal Human Papillomaviruses (HPVs) play a role in the development of a subset of head and neck cancers. Cutaneous HPVs are abundantly present in the oral cavity. The determinants of these infections have not been extensively investigated. We assessed the correlates of oral infection by alpha and beta and/or gamma HPVs in HIV-infected and uninfected men who have sex with men (MSM). Oral rinse-and-gargles were collected with a mouthwash. Alpha and beta/gamma HPVs were detected using the Linear Array HPV genotyping test and a multiplex PCR combined with Luminex technology, respectively. Multiple logistic regression was performed to identify independent predictors of oral HPV infection. Overall, 193 HIV-uninfected and 117 HIV-infected MSM were enrolled. Among HIV-infected MSM, the only determinant of alpha HPV infection was the number of lifetime oral sex partners (AOR: 8.26, 95% CI: 2.26–30.16). The strongest determinant of beta/gamma HPV infection was represented by practicing condomless receptive oral sex (AOR: 10.76, 95% CI: 1.56–74.17). Age was independently associated with alpha HPV infection in HIV-uninfected MSM. Beta/gamma HPV infection was not associated with sexual behavior in these subjects. In conclusion, predictors of oral infection differ between HIV-infected and uninfected MSM, as well as between alpha and beta/gamma HPVs.

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1100National prevalence of oral HPV infection in vaccinated and unvaccinated young adults in Brazil

International Journal of Epidemiology ◽

10.1093/ije/dyab168.707 ◽

2021 ◽

Vol 50 (Supplement_1) ◽

Author(s):

Natália Luiza Kops ◽

Juliana Comerlato ◽

Isabel Bandeira ◽

Marina Bessel ◽

Ana Goretti Kalume Maranhão ◽

...

Keyword(s):

Hpv Vaccination ◽

Hpv Infection ◽

Quadrivalent Vaccine ◽

Sampling Weights ◽

Cross Sectional ◽

Hpv Prevalence ◽

Significant Difference ◽

Oral Hpv ◽

Hpv Types ◽

Putative Precursor

Abstract Background Prophylactic HPV vaccination has been recommended for the prevention of cancers caused by HPV infection. Nevertheless, may be reduce the oral HPV prevalence, the putative precursor to oral squamous cell carcinoma. This study aimed to report the prevalence of oral HPV among vaccinated and unvaccinated women and men aged 16 to 25 years who use the public health system. Methods POP-Brazil study is a cross-sectional, multicentric survey. Participants were recruited from 119 public primary care units distributed throughout all 27 capitals of Brazil. Trained health professionals applied a face-to-face interview. Oral sample was collected through mouthwash and gargle cycles. HPV genotyping was performed in a central lab using the Roche PCR-based Linear Array genotyping test. Sampling weights by sex and age were applied to the data. Results Oral HPV samples were collected from 5,684 participants; 613 (8.93%) vaccinated against HPV, in which 86.57% were women. Among women, the overall HPV prevalence was significantly lower in those vaccinated [0.43% (95% CI, 0.03-0.83)] than unvaccinated [1.65% (95% CI, 0.97– 2.33] (p < 0.01). Among men, no significant difference was found. All vaccinated individuals were negative to the HPV types present in the quadrivalent vaccine (6, 11, 16, and 18). Conclusion Vaccinated individuals had a lower prevalence of overall oral HPV besides the null infection by 6, 11, 16, and 18 HPV types showing another benefit of this cancer prevention measure. Due to the low prevalence of oral HPV, type specific analysis demand higher number of positive participants.

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Prevalence and determinants of oral and cervicogenital HPV infection: baseline analysis of the MHOC cohort study

10.1101/2022.01.15.22269344 ◽

2022 ◽

Author(s):

Andrew Brouwer ◽

Lora P Campredon ◽

Heather M Walline ◽

Brittany M Marinelli ◽

Christine M Goudsmit ◽

...

Keyword(s):

Older Adult ◽

Sexual Behaviors ◽

Hpv Infection ◽

Infection Prevalence ◽

Full Cohort ◽

College Age ◽

Hpv Prevalence ◽

Adult Participants ◽

Ann Arbor ◽

Oral Hpv

We determined baseline oral and cervicogenital human papillomavirus (HPV) prevalence and determinants of infection in the Michigan HPV and Oropharyngeal Cancer (MHOC) study. We enrolled 394 college-age and older-adult participants of both sexes in Ann Arbor, Michigan and the surrounding area. All participants provided an oral sample at baseline, and 130 females provided a cervicogenital sample. Samples were tested for 18 HPV genotypes using polymerase chain reaction (PCR) MassArray. Participants filled out sociodemographic and behavioral questionnaires. Prevalence ratios for HPV oral or cervicogenital prevalence by predictor variables were estimated in univariable log-binomial models. Analysis was conducted 2018-20. In the full cohort, baseline oral HPV prevalence was 10.0% for any detected genotype (among the 338 valid oral tests at baseline) and 6.5% for high-risk types, and cervicogenital prevalence was 20.0% and 10.8%, respectively (among the 130 first valid cervicogenital tests). Oral HPV prevalence did not vary by sex, with 10.5% of women and 9.0% of men having an infection. We found a high prevalence of oral and cervicogenital HPV infection among those reporting no recent sexual partners compared to those with a single recent sexual partner, but prevalence increased with the number of recent partners for most sexual behaviors. We observed an ecological fallacy masking the direction of impact of vaccination on HPV prevalence in the full cohort compared to the college-aged and older-adult populations considered separately. Substance use was not significantly associated with oral or cervicogenital HPV infection. Many studies report substantially higher oral HPV infection prevalence in men than in women. That difference may not be uniform across populations in the US.

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Oral Human Papillomavirus (HPV) Infection in HPV-Positive Patients With Oropharyngeal Cancer and Their Partners

Journal of Clinical Oncology ◽

10.1200/jco.2014.55.1341 ◽

2014 ◽

Vol 32 (23) ◽

pp. 2408-2415 ◽

Cited By ~ 94

Author(s):

Gypsyamber D'Souza ◽

Neil D. Gross ◽

Sara I. Pai ◽

Robert Haddad ◽

Karen S. Anderson ◽

...

Keyword(s):

Human Papillomavirus ◽

General Population ◽

Oropharyngeal Cancer ◽

Quantitative Polymerase Chain Reaction ◽

Invasive Cervical Cancer ◽

Hpv Infection ◽

Hpv Dna ◽

Oral Rinse ◽

Hpv Prevalence ◽

Oral Hpv

Purpose To better understand oral human papillomavirus (HPV) infection and cancer risk among long-term sexual partners of patients with HPV-positive oropharyngeal cancer (HPV-OPC). Patients and Methods An oral rinse sample, risk factor survey, cancer history, and oral examination (partners only) were collected from patients with HPV-OPC and their partners. Oral rinse samples were evaluated for 36 types of HPV DNA using PGMY 09/11 primers and line-blot hybridization and HPV16 copy number using quantitative polymerase chain reaction. Oral HPV prevalence was compared with infection among those age 45 to 65 years using National Health and Nutrition Examination Survey (NHANES) 2009-2010. Results A total of 164 patients with HPV-OPC and 93 of their partners were enrolled. Patients were primarily men (90%), were never-smokers (51%), and had performed oral sex (97%), with a median age of 56 years; they had a high prevalence of oncogenic oral HPV DNA (61%) and oral HPV16 DNA (54%) at enrollment. Female partners had comparable oncogenic oral HPV prevalence compared with members of the general population of the same age (1.2% v 1.3%). Among the six male partners, no oncogenic oral HPV infections were detected. No precancers or cancers were identified during partner oral cancer screening examinations. However, a history of cervical disease was reported by nine partners (10.3%) and two female patients (11.8%), and three patients (2.0%) reported a previous partner who developed invasive cervical cancer. Conclusion Oral HPV16 DNA is commonly detected among patients with HPV-OPC at diagnosis, but not among their partners. Partners of patients with HPV-OPC do not seem to have elevated oral HPV infection compared with the general population.

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