Organic mineral supplementation on differential protein profile of Osmanabadi bucks (Capra hircus)

2021 ◽  
Vol 21 (3) ◽  
pp. 100533
Author(s):  
Backialakshmi Sekar ◽  
Arunachalam Arangasamy ◽  
Sharanya Jeevendra Naidu ◽  
Ippala Janardhan Reddy ◽  
Raghavendra Bhatta
Keyword(s):  
Protein Profile ◽  
Capra Hircus ◽  
Differential Protein ◽  
Mineral Supplementation ◽  
Organic Mineral

Strategies of inorganic and organic trace mineral supplementation in gestating hyperprolific sow diets: effects on the offspring performance and fetal programming

2021 ◽  
Author(s):  
Sandra Villagómez-Estrada ◽  
José F Pérez ◽  
Sandra van Kuijk ◽  
Diego Melo-Durán ◽  
Asal Forouzandeh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mineral Content ◽  
Partial Replacement ◽  
Gut Health ◽  
Trace Mineral ◽  
Offspring Performance ◽  
Mineral Supplementation ◽  
Organic Mineral ◽  
Parity Number ◽  
Inorganic Mineral

Abstract The aim of the present study was to evaluate the effect of trace mineral nutrition on sow performance, mineral content, and intestinal gene expression of neonate piglets when inorganic mineral sources (ITM) were partially replaced by their organic mineral (OTM) counterparts. At 35 d post-mating, under commercial conditions, a total of 240 hyperprolific multiparous sows were allocated into three experimental diets: 1) ITM: with Zn, Cu, and Mn at 80, 15, and 60 mg/kg, respectively; 2) Replace: with a 30 % replacement of ITM by OTM, resulting in ITM + OTM supplementation of Zn (56 + 24 mg/kg), Cu (10.5 + 4.5 mg/kg), and Mn (42 + 18 mg/kg); and 3) Reduce and replace (R&R): reducing a 50 % of the ITM source of Zn (40 + 24 mg/kg), Cu (7.5 + 4.5 mg/kg), and Mn (30 + 18 mg/kg). At farrowing, 40 piglets were selected, based on birth weight (light: < 800 g, and average: > 1,200 g), for sampling. Since the present study aimed to reflect results under commercial conditions, it was difficult to get an equal parity number between the experimental diets. Overall, no differences between experimental diets on sow reproductive performance were observed. Light piglets had a lower mineral content (P < 0.05) and a downregulation of several genes (P < 0.10) involved in physiological functions compared to their average littermates. Neonate piglets born from Replace sows had an upregulation of genes involved in functions like: Immunity and Gut barrier, compared to those born from ITM sows (P < 0.10), particularly in light piglets. In conclusion, the partial replacement of ITM by their OTM counterparts represents an alternative to the totally inorganic supplementation with improvements on neonate piglet gene expression, particularly in the smallest piglets of the litter. The lower trace mineral storage together with the greater downregulation of gut health genes exposed the immaturity and vulnerability of small piglets.

scholarly journals 338EFFECT OF TRACE MINERAL NUTRITION ON FOLLICULAR RESPONSE, OVULATION, AND EMBRYO PRODUCTION IN SUPEROVULATED ANGUS HEIFERS

2004 ◽  
Vol 16 (2) ◽  
pp. 289 ◽  
Author(s):  
G.C. Lamb ◽  
D.R. Brown ◽  
R.C. Wasson ◽  
J.E. Larson ◽  
C.R. Dahlen ◽  
...  
Keyword(s):  
Transrectal Ultrasound ◽  
Latin Square ◽  
Dose Schedule ◽  
Trace Mineral ◽  
Embryo Production ◽  
Mineral Supplement ◽  
Mineral Supplementation ◽  
Des Moines ◽  
Organic Mineral ◽  
Angus Heifers

We determined whether trace mineral supplementation prior to embryo collection affected embryo production and quality. Twenty half-sibling, Angus heifers originating from a common herd were assigned to three treatment groups using a 3×3 latin square design and three rotations of the treatments: (1) heifers received no added mineral to their diet (Control;; n=53); (2) heifers received a commercially available organic mineral supplement (Organic;; Albion Cattle Breeder Pak, Des Moines, IA, USA;; n=52); and (3) heifers received an isomineral, all inorganic mineral supplement (Inorganic;; Inorganic Breeder Pak, Albion, Des Moines, IA, USA;; n=55). All heifers had ad libitum access to hay and were fed a supplement containing corn and soybean meal. Heifers received a 25-mg injection of PGF on Day −23 at which point individual feeding of the corn/soybean/mineral supplement was initiated and fed at recommended levels until the day of embryo collection. All heifers were monitored for signs of estrus, but regardless, all heifers received a 1-mg injection of estradiol cypionate (ECP;; Pharmacia, Kalamazoo, MI, USA) and a CIDR (Pharmacia) on Day −16. From Day −12 to Day −8 heifers received 29mg of follicle stimulating hormone (pFSH, batch 9109, Sioux Biochemical, Sioux Center, IA, USA) in a twice daily decreasing dose schedule. On Day −9 heifers received two 5-mg injections of PGF (AM and PM) and the CIDR was removed in the PM. All heifers were inseminated artificially at 36, 48, and 60h after CIDR removal. On Day 0, embryos were recovered using a nonsurgical procedure and were evaluated under a stereomicroscope. Heifers were given a 45-day adaption period of no mineral supplementation before initiating a new treatment as above. On Days −12, −7, and 0, ovaries were scanned via transrectal ultrasound to determine the presence and number of follicles and CL in each ovary. There were no treatment differences in the number of heifers with a CL on Day −12, the total number of follicles on Day −7, or the total numbers of CL and unovulated follicles on Day 0. Although the total number of recovered ova/embryos were similar among groups (4.2±0.6, 3.6±0.6, and 3.3±0.6 for Control, Inorganic, and Organic heifers, respectively), the number of unfertilized oocytes was greater (P<0.05) in Inorganic (2.3±0.4) than in Organic (0.8±0.4) heifers, whereas Control heifers were intermediate (1.3±0.4). In addition, Control heifers had a greater (P<0.10) number of degenerate embryos (0.9±0.2) than Organic (0.3±0.2) or Inorganic (0.3±0.2) heifers. Organic heifers produced a greater number (P<0.10) of transferable embryos (2.2±0.4) than Inorganic heifers (1.1±0.4), with Control heifers intermediate (2.0±0.4). We conclude that heifer and mineral rotation accounted for the greatest differences in embryo production and quality. However, feeding an Organic mineral tended to increase the production of transferable embryos in purebred Angus heifers.

Semen variables and sperm membrane protein profile of Saanen bucks (Capra hircus) in dry and rainy seasons of the northeastern Brazil (3°S)

2014 ◽  
Vol 59 (5) ◽  
pp. 561-573 ◽  
Author(s):  
M. F. van Tilburg ◽  
M. G. F. Salles ◽  
M. M. Silva ◽  
R. A. Moreira ◽  
F. B. Moreno ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Profile ◽  
Northeastern Brazil ◽  
Capra Hircus ◽  
Sperm Membrane ◽  
Dry And Rainy Seasons

scholarly journals Differential protein profile in sexed bovine semen: shotgun proteomics investigation

2014 ◽  
Vol 10 (6) ◽  
pp. 1264-1271 ◽  
Author(s):  
Michele De Canio ◽  
Alessio Soggiu ◽  
Cristian Piras ◽  
Luigi Bonizzi ◽  
Andrea Galli ◽  
...  
Keyword(s):  
Protein Profile ◽  
Shotgun Proteomics ◽  
Differential Protein ◽  
Bovine Semen

scholarly journals Differential Protein Abundance in Dark-Cutting and Normal-pH Beef

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
F. Kiyimba ◽  
S. Hartson ◽  
J. Rogers ◽  
G. Mafi ◽  
D. VanOverbeke ◽  
...  
Keyword(s):  
Protein Expression ◽  
Pathway Analysis ◽  
Solid Phase ◽  
Muscle Protein ◽  
Expression Profiles ◽  
Protein Profile ◽  
Differentially Expressed ◽  
Cutting Condition ◽  
Differentially Expressed Proteins ◽  
Differential Protein

ObjectivesDark-cutting beef is a meat quality defect in which meat does not display the marketable bright-red color. Although previous studies have indicated that the ultimate pH of dark-cutting beef is greater than normal, the mechanistic basis for the occurrence is not clear. Various mitochondrial and glycolytic enzymes/proteins are involved in muscle metabolism and lowering of pH. However, limited knowledge is currently available on the muscle protein profile differences between dark-cutting and normal-pH beef. The objective of the current study was to identify proteins related to the development of the dark-cutting condition by comparing the protein expression differences between dark-cutting and normal-pH beef.Materials and MethodsDark-cutting and normal-pH beef samples were collected from six (n = 6) different animals after slaughter. Tissue samples (0.5 g) were digested in 5 mL of lysis buffer. Tissue lysates were homogenized, boiled, sonicated using a bioruptor and centrifuged at 10,000 g for 10 min. Samples were digested with trypsin/Lys-C overnight at 37°C, after which additional 2 µg/mL of protease was added and digestion was continued for another 8h. The resulting trypsinolytic peptides were acidified to 1% trifluoroacetic acid and purified by solid phase extraction with C18 affinity media. Protein expression profiles of both dark-cutting and normal-pH beef samples were determined using LC-MS/MS mass spectrometry-based proteomics. Collected raw data instrument files were searched against a bovine proteome database of 23,968 bovine proteome sequences using MaxQuant (V.1.5.3.8). Differential protein expression analysis was done in Perseus (V.1.5.1.3). Ingenuity pathway analysis (IPA) was utilized to determine the significant pathways of the differentially expressed proteins in dark-cutting and normal-pH beef. Gene ontology enrichment pathway analysis was performed to determine the main functions of the differentially expressed proteins in dark-cutting and normal-pH beef identified in our samples.ResultsMass spectrometry analysis identified 1148 proteins, and 97 of these proteins were differentially expressed between normal-pH and dark-cutting beef (P < 0.05). Fold change of 1.5 was observed for 29 proteins. Dark-cutting beef had 19 abundant proteins, while normal-pH beef had 10 abundant proteins. The majority of the upregulated proteins in dark-cutting beef were involved in mitochondrial functioning and metabolism, while the majority of the downregulated proteins were important in glycogen degradation, calcium signaling, α-adrenergic signaling, n-NOS-signaling and the proteasome pathways.ConclusionThe results identify new protein biomarkers associated with dark-cutting and suggest new mechanistic explanations for the dark-cutting phenotype.

scholarly journals Peripheral blood proteomic profiling of idiopathic pulmonary fibrosis biomarkers in the multicentre IPF-PRO Registry

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Jamie L. Todd ◽  
◽  
Megan L. Neely ◽  
Robert Overton ◽  
Katey Durham ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Protein Expression ◽  
Lung Disease ◽  
Disease Severity ◽  
Protein Profile ◽  
Receiver Operating Curve ◽  
Intercellular Adhesion Molecule ◽  
Differential Protein Expression ◽  
Differential Protein

Abstract Background Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease for which diagnosis and management remain challenging. Defining the circulating proteome in IPF may identify targets for biomarker development. We sought to quantify the circulating proteome in IPF, determine differential protein expression between subjects with IPF and controls, and examine relationships between protein expression and markers of disease severity. Methods This study involved 300 patients with IPF from the IPF-PRO Registry and 100 participants without known lung disease. Plasma collected at enrolment was analysed using aptamer-based proteomics (1305 proteins). Linear regression was used to determine differential protein expression between participants with IPF and controls and associations between protein expression and disease severity measures (percent predicted values for forced vital capacity [FVC] and diffusion capacity of the lung for carbon monoxide [DLco]; composite physiologic index [CPI]). Multivariable models were fit to select proteins that best distinguished IPF from controls. Results Five hundred fifty one proteins had significantly different levels between IPF and controls, of which 47 showed a |log2(fold-change)| > 0.585 (i.e. > 1.5-fold difference). Among the proteins with the greatest difference in levels in patients with IPF versus controls were the glycoproteins thrombospondin 1 and von Willebrand factor and immune-related proteins C-C motif chemokine ligand 17 and bactericidal permeability-increasing protein. Multivariable classification modelling identified nine proteins that, when considered together, distinguished IPF versus control status with high accuracy (area under receiver operating curve = 0.99). Among participants with IPF, 14 proteins were significantly associated with FVC % predicted, 23 with DLco % predicted, 14 with CPI. Four proteins (roundabout homolog-2, spondin-1, polymeric immunoglobulin receptor, intercellular adhesion molecule 5) demonstrated the expected relationship across all three disease severity measures. When considered in pathways analyses, proteins associated with the presence or severity of IPF were enriched in pathways involved in platelet and haemostatic responses, vascular or platelet derived growth factor signalling, immune activation, and extracellular matrix organisation. Conclusions Patients with IPF have a distinct circulating proteome and can be distinguished using a nine-protein profile. Several proteins strongly associate with disease severity. The proteins identified may represent biomarker candidates and implicate pathways for further investigation. Trial registration ClinicalTrials.gov (NCT01915511).

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