miR-21 differentially regulates IL-1β and IL-10 expression in human decidual cells infected with streptococcus B

Intrauterine transmission of the Chikungunya virus (CHIKV) during early pregnancy has rarely been reported, although vertical transmission has been observed in newborns. Here, we report four cases of spontaneous abortion in women who became infected with CHIKV between the 11th and 17th weeks of pregnancy. Laboratorial confirmation of the infection was conducted by RT-PCR on a urine sample for one case, and the other three were by detection of IgM anti-CHIKV antibodies. Hematoxylin and eosin (H&E) staining and an electron microscopy assay allowed us to find histopathological, such as inflammatory infiltrate in the decidua and chorionic villi, as well as areas of calcification, edema and the deposition of fibrinoid material, and ultrastructural changes, such as mitochondria with fewer cristae and ruptured membranes, endoplasmic reticulum with dilated cisterns, dispersed chromatin in the nuclei and the presence of an apoptotic body in case 1. In addition, by immunohistochemistry (IHC), we found a positivity for the anti-CHIKV antibody in cells of the endometrial glands, decidual cells, syncytiotrophoblasts, cytotrophoblasts, Hofbauer cells and decidual macrophages. Electron microscopy also helped in identifying virus-like particles in the aborted material with a diameter of 40–50 nm, which was consistent with the size of CHIKV particles in the literature. Our findings in this study suggest early maternal fetal transmission, adding more evidence on the role of CHIKV in fetal death.

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Triterpenoid acids from Schisandra propinqua with cytotoxic effect on rat luteal cells and human decidual cells in vitro

Fitoterapia ◽

10.1016/s0367-326x(01)00269-6 ◽

2001 ◽

Vol 72 (4) ◽

pp. 435-437 ◽

Cited By ~ 12

Author(s):

Ye-Gao Chen ◽

Guo-Wei Qin ◽

Lin Cao ◽

Ying Leng ◽

Yu-Yuan Xie

Keyword(s):

Cytotoxic Effect ◽

Luteal Cells ◽

Decidual Cells

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Progesterone-Dependent Induction of Phospholipase C-Related Catalytically Inactive Protein 1 (PRIP-1) in Decidualizing Human Endometrial Stromal Cells

Endocrinology ◽

10.1210/en.2015-1914 ◽

2016 ◽

Vol 157 (7) ◽

pp. 2883-2893 ◽

Cited By ~ 16

Author(s):

Joanne Muter ◽

Paul J. Brighton ◽

Emma S. Lucas ◽

Lauren Lacey ◽

Anatoly Shmygol ◽

...

Keyword(s):

Phospholipase C ◽

Stromal Cells ◽

Nuclear Translocation ◽

Scaffold Protein ◽

Trophoblast Invasion ◽

Differentiation Markers ◽

Endometrial Stromal Cells ◽

Decidual Cells ◽

Phosphoinositide 3 Kinase ◽

Inactive Protein

Decidualization denotes the transformation of endometrial stromal cells into specialized decidual cells. In pregnancy, decidual cells form a protective matrix around the implanting embryo, enabling coordinated trophoblast invasion and formation of a functional placenta. Continuous progesterone (P4) signaling renders decidual cells resistant to various environmental stressors, whereas withdrawal inevitably triggers tissue breakdown and menstruation or miscarriage. Here, we show that PLCL1, coding phospholipase C (PLC)-related catalytically inactive protein 1 (PRIP-1), is highly induced in response to P4 signaling in decidualizing human endometrial stromal cells (HESCs). Knockdown experiments in undifferentiated HESCs revealed that PRIP-1 maintains basal phosphoinositide 3-kinase/Protein kinase B activity, which in turn prevents illicit nuclear translocation of the transcription factor forkhead box protein O1 and induction of the apoptotic activator BIM. By contrast, loss of this scaffold protein did not compromise survival of decidual cells. PRIP-1 knockdown did also not interfere with the responsiveness of HESCs to deciduogenic cues, although the overall expression of differentiation markers, such as PRL, IGFBP1, and WNT4, was blunted. Finally, we show that PRIP-1 in decidual cells uncouples PLC activation from intracellular Ca2+ release by attenuating inositol 1,4,5-trisphosphate signaling. In summary, PRIP-1 is a multifaceted P4-inducible scaffold protein that gates the activity of major signal transduction pathways in the endometrium. It prevents apoptosis of proliferating stromal cells and contributes to the relative autonomy of decidual cells by silencing PLC signaling downstream of Gq protein-coupled receptors.

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Oxytocin Inhibits T-Type Calcium Current of Human Decidual Stromal Cells

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.2005-0480 ◽

2005 ◽

Vol 90 (7) ◽

pp. 4191-4197 ◽

Cited By ~ 2

Author(s):

Bo Liu ◽

Stephen J. Hill ◽

Raheela N. Khan

Keyword(s):

Stromal Cells ◽

Calcium Current ◽

Elective Cesarean Section ◽

Dependent Manner ◽

Uterine Contractility ◽

Patch Clamp Technique ◽

Concentration Dependent Manner ◽

Inactivation Curve ◽

Decidual Cells ◽

Elective Cesarean

Abstract Context: Little is known about the crosstalk between the decidua and myometrium in relation to human labor. The hormone oxytocin (OT) is considered to be a key mediator of uterine contractility during parturition, exerting some of its effects through calcium channels. Objective: The objective was to characterize the effect of OT on the T-type calcium channel in human decidual stromal cells before and after the onset of labor. Design: The nystatin-perforated patch-clamp technique was used to record inward T-type calcium current (ICa(T)) from acutely dispersed decidual stromal cells obtained from women at either elective cesarean section [CS (nonlabor)] or after normal spontaneous vaginal delivery [SVD (labor)]. Setting: These studies took place at the University of Nottingham Medical School. Results: I Ca(T) of both SVD and CS cells were blocked by nickel (IC50 of 5.6 μm) and cobalt chloride (1 mm) but unaffected by nifedipine (10 μm). OT (1 nm to 3.5 μm) inhibited ICa(T) of SVD cells in a concentration-dependent manner, with a maximal inhibition of 79.0% compared with 26.2% in decidual cells of the CS group. OT-evoked reduction of ICa(T) was prevented by preincubation with the OT antagonist L371,257 in the SVD but not CS group. OT, in a concentration-dependent manner, displaced the steady-state inactivation curve for ICa(T) to the left in the SVD group with no significant effect on curves of the CS group. Conclusion: Inhibition of ICa(T) by OT in decidual cells obtained during labor may signify important functional remodeling of uterine signaling during this period.

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Lipocortin-I Inhibits the Synthesis and Release of Prolactin from Human Decidual Cells: Evidence for Autocrine/Paracrine Regulation by Lipocortin-I*

Endocrinology ◽

10.1210/endo-128-2-1123 ◽

1991 ◽

Vol 128 (2) ◽

pp. 1123-1128 ◽

Cited By ~ 19

Author(s):

CATHERINE PIHOKER ◽

ROBERT J. FEENEY ◽

JUI-LAN SU ◽

STUART HANDWERGER

Keyword(s):

Paracrine Regulation ◽

Decidual Cells

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Increased Phosphorylation of Myosin Light Chain Prevents in Vitro Decidualization

Endocrinology ◽

10.1210/en.2006-1673 ◽

2007 ◽

Vol 148 (7) ◽

pp. 3176-3184 ◽

Cited By ~ 21

Author(s):

Ivanna Ihnatovych ◽

WenYang Hu ◽

Jody L. Martin ◽

Asgerally T. Fazleabas ◽

Primal de Lanerolle ◽

...

Keyword(s):

Light Chain ◽

Myosin Light Chain ◽

Smooth Muscle Actin ◽

Myosin Ii ◽

Active Form ◽

Adenoviral Infection ◽

Decidual Cells ◽

Cytoskeletal Dynamics ◽

Mlc20 Phosphorylation

Differentiation of stromal cells into decidual cells, which is critical to successful pregnancy, represents a complex transformation requiring changes in cytoskeletal architecture. We demonstrate that in vitro differentiation of human uterine fibroblasts into decidual cells includes down-regulation of α-smooth muscle actin and β-tubulin, phosphorylation of focal adhesion kinase, and redistribution of vinculin. This is accompanied by varied adhesion to fibronectin and a modified ability to migrate. Cytoskeletal organization is determined primarily by actin-myosin II interactions governed by the phosphorylation of myosin light chain (MLC20). Decidualization induced by cAMP [with estradiol-17β (E) and medroxyprogesterone acetate (P)] results in a 40% decrease in MLC20 phosphorylation and a 55% decline in the long (214 kDa) form of myosin light-chain kinase (MLCK). Destabilization of the cytoskeleton by inhibitors of MLCK (ML-7) or myosin II ATPase (blebbistatin) accelerates decidualization induced by cAMP (with E and P) but inhibits decidualization induced by IL-1β (with E and P). Adenoviral infection of human uterine fibroblast cells with a constitutively active form of MLCK followed by decidualization stimuli leads to a 30% increase in MLC20 phosphorylation and prevents decidualization. These data provide evidence that the regulation of cytoskeletal dynamics by MLC20 phosphorylation is critical for decidualization.

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Differential expression of the ED sequence-containing form of cellular fibronectin in embryonic and adult human tissues

Journal of Cell Science ◽

10.1242/jcs.88.4.419 ◽

1987 ◽

Vol 88 (4) ◽

pp. 419-430

Author(s):

T. Vartio ◽

L. Laitinen ◽

O. Narvanen ◽

M. Cutolo ◽

L.E. Thornell ◽

...

Keyword(s):

Fusion Protein ◽

Antigenic Determinant ◽

Solid Phase ◽

Basement Membranes ◽

Malignant Tumours ◽

Embryonic Tissues ◽

Adult Tissues ◽

Decidual Cells ◽

Foetal Kidney ◽

Cellular Fibronectin

Monoclonal mouse hybridoma antibodies were obtained for secreted cellular fibronectin (cFn) from A8387 fibrosarcoma cells. One of them, 52-DH1 (DH), reacted exclusively with cFns but not with plasma Fns (pFns) in immunoblotting and solid-phase EIA. The DH antibody also recognized thermolysin cFn fragments and beta-galactosidase-Fn fusion protein which contained the ED sequence specific to at least some forms of cFns. On the other hand, the DH antibody failed to recognize a fusion protein that was otherwise identical but lacked the ED sequence. Thus, the antigenic determinant for the DH antibody was located to the ED sequence. The DH antibody was then used to study the expression of ED sequence containing cFn (EcFn). For comparisons, another monoclonal antibody, 52BF12 (BF), recognizing equally well both pFns and cFns, was used. Immunoblotting of pFn fragments indicated that this antibody had the antigenic determinant at or close to the cell-binding site of Fn. EcFn was revealed by the DH antibody in embryonic and adult fibroblasts and in a variety of other cultured normal and malignant human cells. In embryonic tissues EcFn was abundant in developing basement membranes, as shown in foetal kidney and muscle, while in adult tissues it was confined only to endothelia of larger blood vessels. Furthermore, in embryonic tissues the capillaries showed bright EcFn-positivity not found any more in adult tissues. Human plasma contained a small quantity of EcFn, which may hence have an endothelial origin. EcFn was also prominent in the stroma of malignant tumours as well as in reactive benign conditions, such as granulation tissue and decidual cells. The results suggest that EcFn is a form of the protein which may have a particular role in developing and reactive tissues in embryos and adults.

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