HSF1 and GM-CSF expression, its association with cardiac health, and assessment of organ function during heat stress in crossbred Jersey cattle

Abstract Background Extreme panting under heat stress threatens dairy cattle milk production. Previous research has revealed that the gas exchange-mediated respiratory drive in critically ill dairy cattle with low O2 saturation induces panting. Vascular endothelial growth factor (VEGF) signaling may play important roles in immunosuppression and oxidative stress during severe respiratory stress responses in heat-stressed cattle. The objectives of this study were to transcriptomically analyze mRNA expression mediating heat-induced respiratory stress-associated panting, evaluate gas exchange, screen hub genes, and verify the expression of proteins encoded by differentially expressed genes in lymphocyte pathways. Results Jersey cattle were naturally heat-exposed. Physiological data were collected for response evaluation, and blood was collected for gas exchange and gene expression assays at 06:00, 10:00 and 14:00 continuously for 1 week. Lymphocytes were isolated from whole-blood samples for mRNA-seq and expression analysis of key pathway genes/proteins. The cattle respiration rates differed with time, averaging 51 bpm at 06:00, 76 bpm at 10:00, and 121 bpm at 14:00 (p < 0.05). Gas exchange analysis showed that both pH and pCO2 differed with time: they were 7.41 and 41 mmHg at 06:00, 7.45 and 37.5 mmHg at 10:00, and 7.49 and 33 mmHg at 14:00, respectively (p < 0.01). Sixteen heat-related differentially expressed genes (DEGs; 13 upregulated and 3 downregulated) were screened between 212 DEGs and 1370 heat stress-affected genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) hub gene functional analysis annotated eleven genes to signal transduction, six genes to the immune response, and five genes to the endocrine response, including both prostaglandin-endoperoxide synthase 2 (PTGS2) and VEGF. Gene Ontology (GO) functional enrichment analysis revealed that oxygen regulation was associated with the phosphorus metabolic process, response to oxygen levels, response to decreased oxygen levels, response to hypoxia and cytokine activity terms. The main signaling pathways were the VEGF, hypoxia inducible factor-1(HIF-1), cytokine-cytokine receptor interaction and TNF pathways. Four genes involved Integrin beta 3 (ITBG3), PTGS2, VEGF, and myosin light chain 9 (MYL9) among the 16 genes related to immunosuppression, oxidative stress, and endocrine dysfunction were identified as participants in the VEGF signaling pathway and oxygenation. Conclusion These findings help elucidate the underlying immune and oxygen regulation mechanisms associated with the VEGF signaling pathway in heat-stressed dairy cattle.

Download Full-text

TALL FESCUE TOXICITY: AN INVESTIGATION OF IDIOPATHIC BOVINE HYPERTHERMIA (IBH) IN THE NORTH AUCKLAND PENINSULA.

Proceedings of the New Zealand Grassland Association ◽

10.33584/jnzg.1986.47.1723 ◽

1986 ◽

pp. 183-186

Author(s):

M.P. Kearns

Keyword(s):

Heat Stress ◽

Dairy Cattle ◽

Tall Fescue ◽

Management Practices ◽

Farm Management ◽

Dairy Herds ◽

Jersey Cattle ◽

The North ◽

Almost All

A survey was made of 64 dairy herds, grazing pastures containing naturalised tall fescue in the North Auckland peninsula, to demonstrate an assooation of endophyte fungus in tall fescue with IBH. Of these, 40 farms had cattle with IBH and 24 had no IBH. Endophyte fungus was identtfied in tall fescue samples from all survey farms. Acremonium coenophiolum was demonstrated in samples from almost all farms and a high proportion of these also showed a Phiafophora-like endophyte. No correlation could be shown between the presence of endophyte or any farm management practices such as topdressing etc and IBH. Breed susceptibility was observed in Friesian breeds whilst Jersey cattle were less susceptible. IBH first appeared in November and December with a peak m January. Most affected herds had a hlstory of IBH for many years. Keywords: Dairy cattle, tall fescue toxicity, endophyte fungus, idiopathic bovine hyperthermia, IBH. heat-stress, hyperthermia.

Download Full-text

Involvement of the VEGF Signaling Pathway in Immunosuppression and Hypoxia Stress: Analysis of mRNA Expression in Lymphocytes Mediating Panting in Jersey Cattle under Heat Stress

10.21203/rs.3.rs-272052/v1 ◽

2021 ◽

Author(s):

jian wang ◽

Yang Xiang ◽

Shisong Jiang ◽

Hongchang Li ◽

Caviezel Flurin ◽

...

Keyword(s):

Oxidative Stress ◽

Heat Stress ◽

Gas Exchange ◽

Dairy Cattle ◽

Mrna Expression ◽

Signaling Pathway ◽

Oxygen Regulation ◽

Vegf Signaling ◽

Jersey Cattle ◽

Vegf Signaling Pathway

Abstract Background: Extreme panting under heat stress threatens dairy cattle milk production. Previous research has revealed that the gas exchange-mediated respiratory drive in critically ill dairy cattle with low O2 saturation induces panting. Vascular endothelial growth factor (VEGF) signaling may play important roles in immunosuppression and oxidative stress during severe respiratory stress responses in heat-stressed cattle. The objectives of this study were to transcriptomically analyze mRNA expression mediating heat-induced respiratory stress-associated panting, evaluate gas exchange, screen hub genes, and verify the expression of proteins encoded by differentially expressed genes in lymphocyte pathways. Results: Jersey cattle were naturally heat-exposed. Physiological data were collected for response evaluation, and blood was collected for gas exchange and gene expression assays at 06:00, 10:00 and 14:00 continuously for one week. Lymphocytes were isolated from whole-blood samples for mRNA-seq and expression analysis of key pathway genes/proteins. The cattle respiration rates differed with time, averaging 51 bpm at 06:00, 76 bpm at 10:00, and 121 bpm at 14:00 (p < 0.05). Gas exchange analysis showed that both pH and pCO2 differed with time: they were 7.41 and 41 mmHg at 06:00, 7.45 and 37.5 mmHg at 10:00, and 7.49 and 33 mmHg at 14:00, respectively (p < 0.01). Sixteen heat-related differentially expressed genes (DEGs; 13 upregulated and 3 downregulated) were screened between 212 DEGs and 1370 heat stress-affected genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) hub gene functional analysis annotated eleven genes to signal transduction, six genes to the immune response, and five genes to the endocrine response, including both prostaglandin-endoperoxide synthase 2 (PTGS2) and VEGF. Gene Ontology(GO) functional enrichment analysis revealed that oxygen regulation was associated with the phosphorus metabolic process, response to oxygen levels, response to decreased oxygen levels, response to hypoxia and cytokine activity terms. The main signaling pathways were the VEGF, hypoxia inducible factor-1(HIF-1), cytokine-cytokine receptor interaction and TNF pathways. Four genes involved Integrin beta 3 (ITBG3), PTGS2, VEGF, and myosin light chain 9 (MYL9) among the 16 genes related to immunosuppression, oxidative stress, and endocrine dysfunction were identified as participants in the VEGF signaling pathway and oxygenation. Conclusion: These findings help elucidate the underlying immune and oxygen regulation mechanisms associated with the VEGF signaling pathway in heat-stressed dairy cattle.

Download Full-text