A new colorimetric and fluorescent probe based on Rhodamine B hydrazone derivatives for cyanide and Cu2+ in aqueous media and its application in real life

2019 ◽  
Vol 219 ◽  
pp. 297-306 ◽  
Author(s):  
Chen Long ◽  
Jing-Han Hu ◽  
Qing-Qing Fu ◽  
Peng-Wei Ni
Keyword(s):  
Fluorescent Probe ◽  
Rhodamine B ◽  
Aqueous Media ◽  
Real Life

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

scholarly journals A Biocompatible Colorimetric Triphenylamine- Dicyanovinyl Conjugated Fluorescent Probe for Selective and Sensitive Detection of Cyanide Ion in Aqueous Media and Living Cells

Sensors ◽  
2017 ◽  
Vol 17 (2) ◽  
pp. 405 ◽  
Author(s):  
Zi-Hua Zheng ◽  
Zhi-Ke Li ◽  
Lin-Jiang Song ◽  
Qi-Wei Wang ◽  
Qing-Fei Huang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Aqueous Media ◽  
Living Cells ◽  
Sensitive Detection ◽  
Cyanide Ion

Colorimetric and Fluorescent Probe for Hypochlorite with High Selectivity

2013 ◽  
Vol 295-298 ◽  
pp. 475-478 ◽  
Author(s):  
Zhi Xiang Han ◽  
Ming Hui Du ◽  
Guo Xi Liang ◽  
Xiang Yang Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Rhodamine B ◽  
High Selectivity ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Turn On

Rhodamine B thiohydrazide (RBS) was firstly employed as turn-on fluorescent probe for hypochlorite in aqueous solution and living cells. It exhibits a stable response to hypochlorite from 1.0×10-6to 1.0×10-5M with a detection limit of 3.3×10-7M. The response of this probe to hypochlorite is fast and highly selective compared with other reactive oxygen species (such as.OH,1O2, H2O2) and other common anions (such as X-, ClO2-, ClO4-, NO3-, NO2-, OH-, Ac-, CO32-, SO42-).

Simultaneous Quantification of Hg2+and MeHg+in Aqueous Media with a Single Fluorescent Probe by Multiplexing in the Time Domain

2014 ◽  
Vol 86 (23) ◽  
pp. 11919-11924 ◽  
Author(s):  
Ziqian Zhang ◽  
Baoyan Zhang ◽  
Xuhong Qian ◽  
Zhong Li ◽  
Zhiping Xu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Time Domain ◽  
Aqueous Media ◽  
Simultaneous Quantification ◽  
The Time Domain

scholarly journals Visible Light Induced Enhanced Photocatalytic Degradation of Industrial Effluents (Rhodamine B) in Aqueous Media Using TiO2Nanoparticles

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
J. O. Carneiro ◽  
A. P. Samantilleke ◽  
P. Parpot ◽  
F. Fernandes ◽  
M. Pastor ◽  
...  
Keyword(s):  
Visible Light ◽  
Rhodamine B ◽  
Colloidal Stability ◽  
Titanium Dioxide Nanoparticles ◽  
Aqueous Media ◽  
Research Work ◽  
Industrial Effluents ◽  
Intermediate Compound ◽  
X Ray Diffraction ◽  
Textile Materials

In recent years, new textile materials have been developed through the use of nanotechnology-based tools. The development of textile surfaces with self-cleaning properties has a large combined potential to reduce the environmental impact related to pollution. In this research work, three types of textiles substrates (cotton, Entretela, and polylactic acid (PLA)) were functionalized with titanium dioxide nanoparticles (TiO2) using chemical and mechanical processes (padding). During the functionalization process, two different methods were used, both of which allowed a good fixation of nanoparticles of TiO2on textile substrates. The samples were examined for morphology and for photocatalytic properties under visible light irradiation. A study aimed at evaluating the effect of pH of the aqueous solution of TiO2nanoparticles was performed in order to promote interaction between TiO2and the dye solution rhodamine B (Rh-B). The TiO2nanoparticles were characterized by X-ray diffraction (XRD). The measurement of the zeta potential of the TiO2nanoparticle solution proved to be always positive and have low colloidal stability. Chromatography (HPLC and GC-MS) analyses confirm that oxalic acid is the intermediate compound formed during the photodegradation process.

A “turn-on” fluorescent and colorimetric chemodosimeter for selective detection of Au3+ ions in solution and in live cells via Au3+-induced hydrolysis of a rhodamine-derived Schiff base

2020 ◽  
Vol 44 (19) ◽  
pp. 7954-7961
Author(s):  
Sanchita Mondal ◽  
Saikat Kumar Manna ◽  
Sudipta Pathak ◽  
Aritri Ghosh ◽  
Pallab Datta ◽  
...  
Keyword(s):  
Schiff Base ◽  
Rhodamine B ◽  
Aqueous Media ◽  
Sensitive Detection ◽  
Live Cells ◽  
Selective Detection ◽  
Turn On ◽  
Hydrolysis Of

A chromogenic and “off–on” fluorogenic chemodosimeter (L) based on a naphthalene–rhodamine B derivative was designed, synthesized and characterized for the selective and sensitive detection of Au3+ ions in mixed acetonitrile aqueous media.

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