Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits

2009 ◽  
Vol 122 (3) ◽  
pp. 409-416 ◽  
Author(s):  
M.K. Biswas ◽  
M. Dutt ◽  
U.K. Roy ◽  
R. Islam ◽  
M. Hossain
Keyword(s):  
Somaclonal Variation ◽  
Horticultural Traits

scholarly journals Somaclonal variation on in vitro callus culture potato cultivars

2004 ◽  
Vol 22 (2) ◽  
pp. 300-304 ◽  
Author(s):  
Patricia N. Bordallo ◽  
Derly H. Silva ◽  
José Maria ◽  
Cosme D. Cruz ◽  
Elizabeth P. Fontes
Keyword(s):  
Somaclonal Variation ◽  
Culture Media ◽  
Callus Formation ◽  
Potato Cultivars ◽  
Synthetic Seed ◽  
Arbitrary Sequence ◽  
Stem Explants ◽  
Factors Affecting ◽  
High Level

Synthetic seeds can be an alternative for those species in which botanical seeds are not viable. One of the major problems of in vitro plant cultivation is the high level of somaclonal variation. The most common factors affecting somaclonal variation are genotype, explant source, in vitro period and cultivation conditions in which the culture is established. In this work, calli were induced using leaf and stem explants of the commercial potato cultivars Achat, Baraka, Baronesa, Bintje, and Contenda in MS culture media supplemented with 1.65 mM of picloram and 11.5 mM of 2,4-D. Seventy and 90 days after induction, DNA samples of 40 calli were compared concerning the effects of the two explant (leaf and stem) and two growth regulator sources on five potatoes cultivars. A total of 20 arbitrary sequence primers were evaluated. The RAPD pattern generated by these primers suggested a high percentage of polymorphic fragments among the five genotypes, indicating a high level of genetic variation among cultivars. Cultivar Baronesa showed the highest number of polymorphic fragments for all treatments. The cultivar Contenda showed the smallest somaclonal variation, for most of the treatments, except for the treatment which consisted of stem explants, picloram (1.65 mM) application, and a 70-day period of callus formation. 'Contenda' is, therefore, the most suitable cultivar for synthetic seed production.

scholarly journals A Somaclonal Variant of Rose-Scented Geranium (Pelargonium spp.) with Moderately High Content of Isomenthone in its Essential Oil

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700
Author(s):  
Swaroop S Kulkarni ◽  
Nagawara S Ravindra ◽  
Kalavagunta V N S Srinivas ◽  
Raghavendra N Kulkarni
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Somaclonal Variation ◽  
Genetic Improvement ◽  
In Vitro Mutagenesis ◽  
Conventional Breeding ◽  
Parental Clone ◽  
First Report ◽  
Somaclonal Variant

Rose-scented geranium ( Pelargonium spp.), which is highly valued for its essential oil, is exclusively propagated vegetatively. Hence no genetic improvement work is possible through conventional breeding. Somaclonal variation was generated with and without in vitro mutagenesis using N-nitroso- N-methyl urea (NMU) in an Indian cultivar ‘Bourbon’, and a clone ‘Narmada’. A somaclonal variant (N75) with a moderately high content of isomenthone in its essential oil was isolated from somaclones generated after treatment of internodal explants of clone, ‘Narmada’ with 0.25 mM NMU for 1 h. The contents of isomenthone in its essential oil were 26% and 35%, respectively, in SC2/VM2 and SC3/VM3 generations (second and third vegetative generations, respectively, after in vitro mutagen treatment) as compared with 0.7% and 0.3%, respectively, in the parental clone, ‘Narmada’. The contents of alcohols and their esters (linalool, citronellol, geraniol, citronellyl formate and geranyl formate) in the essential oil of N75 in SC2/VM2 and SC3/VM3 generations were 49% and 35%, respectively, as compared with 69% and 63%, respectively, in the parental clone, ‘Narmada’. This is the first report on a chemovariant of rose-scented geranium with a moderately high content of isomenthone. All earlier reported isomenthone-rich variants of rose-scented geranium had quite high contents of isomenthone (64-71%) in their essential oils. The probable modes of origin of this somaclonal variant, its parental clone ‘Narmada’ (with very low content of isomenthone) and four earlier reported isomenthone-rich variants of Indian cultivars of geranium are discussed.

Micropropagation and Somaclonal Variation of Tulipa suaveolens (Liliaceae) in vitro

2019 ◽  
Vol 50 (4) ◽  
pp. 209-215 ◽  
Author(s):  
T. A. Kritskaya ◽  
A. S. Kashin ◽  
M. Yu. Kasatkin
Keyword(s):  
Somaclonal Variation

Somaclonal variation for genetic improvement in grasspea (Lathyrus sativus L.)

2015 ◽  
Author(s):  
Swapan K. Tripathy ◽  
A. Panda ◽  
Pramod K. Nayak ◽  
Sasmita Dash ◽  
D. Lenka ◽  
...  
Keyword(s):  
Somaclonal Variation ◽  
Chromosomal Abnormalities ◽  
Agronomic Traits ◽  
Flower Colour ◽  
Lathyrus Sativus ◽  
Broad Leaf ◽  
Developmental Variations ◽  
High Seed Yield

Somaclonal variation was revealed among regenerants in four genotypes (Nirmal, P24, Nayagarh local and Dhenkanal local) of grasspea (<italic>Lathyrus sativus</italic> L.) following long term in vitro culture of internode explants. Most of the somaclones which survived till maturity had revealed chromosomal abnormalities and variation in one or more morphological and/or agronomic traits. Some of the variant phenotypes especially those relating to developmental variations were not inherited to R2 generation owing to elimination of chromosomal aberrations that preclude normal morphogenesis. Somaclones with variation in flower colour, seed colour, leaflet length and breadth, foliage and pod pigmentation may be used as genetic markers in breeding <italic>Lathyrus sativus</italic>. Besides, variants with broad leaf, dwarf height, long pod, large seed, short duration and synchronous maturity are agronomically desirable. A large seeded somaclone NGOG 5 having high seed yield and low neurotoxin content (ODAP) recovered in this pursuit, can be a desirable candidate for future breeding programme.

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