Assessing the functional role of color-related CaMYB gene under cold stress using virus-induced gene silencing in the fruit of pepper (Capsicum annuum L.)

2020 ◽  
Vol 272 ◽  
pp. 109504
Author(s):  
Rui-Xing Zhang ◽  
Guo-Xin Cheng ◽  
Gui-Ting Liu ◽  
Si-Yu Chen ◽  
Saeed ul Haq ◽  
...  
2013 ◽  
Vol 12 (3) ◽  
pp. 2492-2506 ◽  
Author(s):  
J.-E. Wang ◽  
D.-W. Li ◽  
Z.-H. Gong ◽  
Y.-L. Zhang

2021 ◽  
Vol 12 ◽  
Author(s):  
Dionis Borràs ◽  
Lorenzo Barchi ◽  
Karina Schulz ◽  
Andrea Moglia ◽  
Alberto Acquadro ◽  
...  

Capsicum annuum L. is one of the most cultivated Solanaceae species, and in the open field, water limitation leading to drought stress affects its fruit quality, fruit setting, fruit size and ultimately yield. We identified stage-specific and a common core set of differentially expressed genes, following RNA-seq transcriptome analyses of a breeding line subjected to acute drought stress followed by recovery (rewatering), at three stages of plant development. Among them, two NAC transcription factor (TF) genes, i.e., CaNAC072 and CaNAC104, were always upregulated after drought stress and downregulated after recovery. The two TF proteins were observed to be localized in the nucleus following their transient expression in Nicotiana benthamiana leaves. The expression of the two NACs was also induced by NaCl, polyethylene glycol (PEG) and abscisic acid (ABA) treatments, suggesting that CaNAC072 is an early, while CaNAC104 is a late abiotic stress-responsive gene. Virus-induced gene silencing (VIGS) of CaNAC104 did not affect the pepper plantlet’s tolerance to drought stress, while VIGS of CaNAC072 increased drought tolerance. Heterologous expression of CaNAC072 in Arabidopsis thaliana as well as in plants mutated for its homolog ANAC072 did not increase drought stress tolerance. This highlights a different role of the two NAC homologs in the two species. Here, we discuss the complex role of NACs as transcriptional switches in the response to drought stress in bell pepper.


2019 ◽  
Vol 249 ◽  
pp. 280-288 ◽  
Author(s):  
M.L. Fiasconaro ◽  
M.E. Lovato ◽  
M.C. Antolín ◽  
L.A. Clementi ◽  
N. Torres ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 837
Author(s):  
Alfonso Llanderal ◽  
Pedro García-Caparrós ◽  
María Teresa Lao ◽  
Maria Luz Segura

The aim of this study was to compile DRIS (Diagnosis and Recommendation Integrated System) norms and sufficiency ranges for pepper grown in greenhouses and the determination of the variation in DRIS norms and sufficiency ranges during the different phenological stages of the crop. The DRIS norms and sufficiency ranges were constituted by 367 leaf samples of Capsicum annuum L. which were collected in different phenological stages of the crop (flowering (FL), fruiting (FT), fruit development (FD), and harvesting (HV)). Two sufficiency ranges were developed; one including all leaf samples and the other considering the leaf samples by phenological stages ((FL) (44), (FT) (96), (FD) (100), and (HV) (127)). Furthermore, 10 DRIS norms and CV were calculated from N, P, K, Ca, and Mg. Two ratios of them were calculated in FL (N/P and N/K), two in FD (N/P and Ca/N) and one in HV (N/P) showing low CV, meaning, according to DRIS norms, the essential role of these nutrients in the crop nutrition. Moreover, the sufficiency ranges computed by all database were: N (34–56), P (1.9–4.3), K (30–51), Ca (12–33), and Mg (4.0–15) (mg g−1 DW), respectively. The data obtained in this experiment highlighted the importance of the sensitivity (low CV) of nutrient in the phenological stages such as happened with N.


2021 ◽  
Vol 7 (2) ◽  
pp. 262-271
Author(s):  
I Komang Damar Jaya ◽  
Bambang Budi Santoso ◽  
Jayaputra Jayaputra

The use of high doses of chemical fertilizers in the production of chili (Capsicum annuum L.) has an adverse impact on the environment, especially in dryland. This study aimed to examine the role of cow manure (PKS), goat manure (PKK), chicken manure (PKA), or a combination of two manures in reducing 25% of the requirement for chemical fertilizer on chili plants. One experiment was carried out in the dryland of Gumantar Village, North Lombok Regency, from May to October 2021. Phonska NPK (15-15-15) chemical fertilizer (PK) at a 1,200 kg/ha dose was used as a control. The treatments tested were 75% PK+PKS, 75% PK+PKK, 75% PK+PKA, 75% PK+PKS+PKK, 75% PK+PKS+PKA and 75% PK+PKK+PKA. The dose of manure used in each treatment was 20 tons/ha. All treatments were repeated three times and arranged in a randomized block design. The results showed that the treatment of manure, especially goat manure and chicken manure, increased the available phosphate (P) in the soil. The growth and yield of chili plants were not significantly different in all treatments, which means that manure could replace the role of 25% of the chemical fertilizer used. These results indicate that manure in the long term can reduce the need for chemical fertilizers to produce chili in dryland.  Key words: chili; dryland; manure; chemical fertilizer; nutrition


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