scholarly journals Storm event impacts on in-stream nitrate concentration and discharge dynamics: A comparison of high resolution in-situ measured data with model simulations

2021 ◽  
Vol 755 ◽  
pp. 143406
Author(s):  
Bano Mehdi ◽  
Christoph Schürz ◽  
Benedikt Grath ◽  
Karsten Schulz
Keyword(s):  
High Resolution ◽  
Nitrate Concentration ◽  
Measured Data ◽  
Storm Event ◽  
Model Simulations ◽  
Stream Nitrate

scholarly journals Spatio-Temporal Variations of Atmospheric NH3 over East Asia by Comparison of Chemical Transport Model Results, Satellite Retrievals and Surface Observations

Atmosphere ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 900 ◽  
Author(s):  
Zhe Wang ◽  
Itsushi Uno ◽  
Kazuo Osada ◽  
Syuichi Itahashi ◽  
Keiya Yumimoto ◽  
...  
Keyword(s):  
High Resolution ◽  
East Asia ◽  
Temporal Variations ◽  
Central China ◽  
Model Simulations ◽  
Satellite Retrievals ◽  
In Situ Observations ◽  
Spatio Temporal ◽  
Surface Observations

Atmospheric ammonia (NH3) plays an important role in the formation of secondary inorganic aerosols, the neutralization of acid rain, and the deposition to ecosystems, but has not been well understood yet, especially over East Asia. Based on the GEOS-Chem model results, the IASI satellite retrievals, the in-site surface observations of a nationwide filter pack (FP) network over Japan and the long-term high resolution online NH3 measurements at Fukuoka of western Japan, the spatio-temporal distributions of atmospheric NH3 over East Asia was analyzed comprehensively. A significant seasonal variation with a summer peak was found in all datasets. Comparison between the satellite retrievals and model simulations indicated that the IASI NH3 vertical column density (VCD) showed good consistency with GEOS-Chem results over North and central China, but had large differences over South China due to the effect of clouds. Over the Japan area, GEOS-Chem simulated NH3 concentrations successfully reproduced the spatio-temporal variations compared with in-situ observations, while IASI NH3 VCD retrievals were below or near the detection limit and difficult to obtain a reasonable correlation for with model results. The comprehensive analysis indicated that there were still some differences among different datasets, and more in-situ observations, improved satellite retrievals, and high-resolution model simulations with more accurate emissions are necessary for better understanding the atmospheric NH3 over East Asia.

The interplay between hydrological flushing and biogeochemical cycling in a 3rd order stream

2021 ◽  
Author(s):  
Magdalena Bieroza ◽  
Ann Louise Heathwaite
Keyword(s):  
Water Quality ◽  
High Resolution ◽  
Critical Role ◽  
Biogeochemical Cycling ◽  
Quality Data ◽  
Storm Event ◽  
Order Stream ◽  
Water Quality Data ◽  
The Impact

<p>High-resolution water quality data obtained with <em>in situ</em> sensors and analysers coupled to flow discharge records can reveal critical information on hydrochemical and biogeochemical functioning of aquatic ecosystems. In this study we explore a rich high-resolution hydrochemical dataset to synthesise the impact of hydrological flushing and biogeochemical cycling on water quality in a 3<sup>rd</sup> order groundwater-fed stream draining an agricultural catchment dominated by grassland.Our results show that despite large storm to storm diversity in hydrochemical responses, storm event magnitude and timing have a critical role in controlling the type of mobilisation, flushing and cycling behaviour. These results can be used to evaluate pollution risks in streams and their effects on freshwater quality.</p>

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

High-Resolution Resistance Mapping in SEM

2018 ◽  
Author(s):  
Grigore Moldovan ◽  
Wolfgang Joachimi ◽  
Guillaume Boetsch ◽  
Jörg Jatzkowski ◽  
Frank Altman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Reference Structure ◽  
Total Resistance ◽  
Embedded Electronics ◽  
Improved Performance ◽  
Mapping Techniques ◽  
Scanning Electron

Abstract This work presents advanced resistance mapping techniques based on Scanning Electron Microscopy (SEM) with nanoprobing systems and the related embedded electronics. Focus is placed on recent advances to reduce noise and increase speed, such as integration of dedicated in situ electronics into the nanoprobing platform, as well as an important transition from current-sensitive to voltagesensitive amplification. We show that it is now possible to record resistance maps with a resistance sensitivity in the 10W range, even when the total resistance of the mapped structures is in the range of 100W. A reference structure is used to illustrate the improved performance, and a lowresistance failure case is presented as an example of analysis made possible by these developments.

scholarly journals Development of the Statistical Model for Monitoring Salinization in the Mekong Delta of Vietnam Using Remote Sensing Data and In-Situ Measurements

Proceedings ◽  
2018 ◽  
Vol 2 (10) ◽  
pp. 565
Author(s):  
Nguyen Nguyen Vu ◽  
Le Van Trung ◽  
Tran Thi Van
Keyword(s):  
Statistical Model ◽  
Mekong Delta ◽  
Remote Sensing Data ◽  
Principal Component ◽  
Measured Data ◽  
In Situ Measurements ◽  
Landsat 8 ◽  
Spectral Bands ◽  
Component Band

This article presents the methodology for developing a statistical model for monitoring salinity intrusion in the Mekong Delta based on the integration of satellite imagery and in-situ measurements. We used Landsat-8 Operational Land Imager and Thermal Infrared Sensor (Landsat- 8 OLI and TIRS) satellite data to establish the relationship between the planetary reflectance and the ground measured data in the dry season during 2014. The three spectral bands (blue, green, red) and the principal component band were used to obtain the most suitable models. The selected model showed a good correlation with the exponential function of the principal component band and the ground measured data (R2 > 0.8). Simulation of the salinity distribution along the river shows the intrusion of a 4 g/L salt boundary from the estuary to the inner field of more than 50 km. The developed model will be an active contribution, providing managers with adaptation and response solutions suitable for intrusion in the estuary as well as the inner field of the Mekong Delta.

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