scholarly journals An Investigation into the Biological Effects of Indirect Potable Reuse Water Using Zebrafish Embryos

2021 ◽  
pp. 147981
Author(s):  
E. Lawton ◽  
P. Antczak ◽  
S. Walker ◽  
E. Germain-Cripps ◽  
F. Falciani ◽  
...  
Keyword(s):  
Biological Effects ◽  
Zebrafish Embryos ◽  
Potable Reuse ◽  
Reuse Water ◽  
Indirect Potable Reuse

Treatment of Trace Organic Compounds by Membrane Processes: At the Lake Arrowhead Water Reuse Pilot Plant

1999 ◽  
Vol 40 (4-5) ◽  
pp. 293-301 ◽  
Author(s):  
Bruno B. Levine ◽  
Kapal Madireddi ◽  
Valentina Lazarova ◽  
Michael K. Stenstrom ◽  
Mel Suffet
Keyword(s):  
Pilot Plant ◽  
Water Reuse ◽  
Ultra Violet ◽  
Treatment Efficiency ◽  
Small Molecular Weight ◽  
Potable Reuse ◽  
Low Concentrations ◽  
Neutral Compounds ◽  
Indirect Potable Reuse ◽  
Trace Organic

Organic and trace organic performance data for ultrafiltration (UF), nanofiltration (NF) and reverse osmosis (RO) at the Lake Arrowhead water reclamation pilot plant are analyzed to determine the treatment efficiency of these processes in an indirect potable reuse design. Four organic parameters were studied: dissolved organic carbon (DOC), ultra-violet absorbance at 254 nm (UV-254), SUVA and base neutral analysis (BNA). UF and NF removed the larger compounds from the influent, but had no significant impact on the base neutral fraction with the exception of sterols. The RO process removed DOC and UV-absorbance compounds from the effluent to their respective detection limits. Base neutral compounds were significantly removed by RO, leaving at extremely low concentrations small molecular weight compounds, indicating indirect potable reuse is technically feasible.

scholarly journals An Australian perspective on DPR: technologies, sustainability and community acceptance

2015 ◽  
Vol 6 (3) ◽  
pp. 355-361 ◽  
Author(s):  
Ian B. Law
Keyword(s):  
Water Supply ◽  
Recent Work ◽  
Western Australia ◽  
Community Education ◽  
Water Recycling ◽  
Potable Reuse ◽  
Community Acceptance ◽  
Indirect Potable Reuse ◽  
Direct Potable Reuse

Australia has had guidelines in place for water recycling (for all uses other than potable reuse) since 2006. These guidelines were extended in May 2008 to cover potable reuse and have since been applied to two potable reuse schemes – one in Brisbane (Queensland) in 2011 and the second in Perth (Western Australia) in 2013. These guidelines cover both indirect potable reuse and direct potable reuse (DPR) and outline the steps that must be followed in the planning and validation of such schemes. This paper summarizes: (i) recent work carried out in Australia on treatment trains and technologies suitable for DPR; (ii) sustainability considerations of DPR and how it compares with other water supply options; and (iii) developments in community education and engagement in the potable reuse space.

The fate of microplastics in natural and engineered aquatic systems: A case study of unplanned indirect potable reuse

2021 ◽  
pp. 100302
Author(s):  
Swadhina Priyadarshini Lenka ◽  
Tahereh Jasemizad ◽  
Iraj Rezapour Balaneji ◽  
Brandon Huang ◽  
Benjamin Campbell ◽  
...  
Keyword(s):  
Aquatic Systems ◽  
Potable Reuse ◽  
Indirect Potable Reuse

scholarly journals Lignans from Bursera fagaroides Affect In Vivo Cell Behavior by Disturbing the Tubulin Cytoskeleton in Zebrafish Embryos

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 8 ◽  
Author(s):  
Mayra Antúnez-Mojica ◽  
Andrés Rojas-Sepúlveda ◽  
Mario Mendieta-Serrano ◽  
Leticia Gonzalez-Maya ◽  
Silvia Marquina ◽  
...  
Keyword(s):  
Cell Migration ◽  
Biological Effects ◽  
In Vitro Models ◽  
In Vivo Model ◽  
Zebrafish Embryos ◽  
Microtubule Cytoskeleton ◽  
Zebrafish Model ◽  
Bioassay Guided Fractionation

By using a zebrafish embryo model to guide the chromatographic fractionation of antimitotic secondary metabolites, seven podophyllotoxin-type lignans were isolated from a hydroalcoholic extract obtained from the steam bark of Bursera fagaroides. The compounds were identified as podophyllotoxin (1), β-peltatin-A-methylether (2), 5′-desmethoxy-β-peltatin-A-methylether (3), desmethoxy-yatein (4), desoxypodophyllotoxin (5), burseranin (6), and acetyl podophyllotoxin (7). The biological effects on mitosis, cell migration, and microtubule cytoskeleton remodeling of lignans 1–7 were further evaluated in zebrafish embryos by whole-mount immunolocalization of the mitotic marker phospho-histone H3 and by a tubulin antibody. We found that lignans 1, 2, 4, and 7 induced mitotic arrest, delayed cell migration, and disrupted the microtubule cytoskeleton in zebrafish embryos. Furthermore, microtubule cytoskeleton destabilization was observed also in PC3 cells, except for 7. Therefore, these results demonstrate that the cytotoxic activity of 1, 2, and 4 is mediated by their microtubule-destabilizing activity. In general, the in vivo and in vitro models here used displayed equivalent mitotic effects, which allows us to conclude that the zebrafish model can be a fast and cheap in vivo model that can be used to identify antimitotic natural products through bioassay-guided fractionation.

Optimizing RO Design Criteria for Indirect Potable Reuse

2011 ◽  
Vol 3 (4) ◽  
pp. 40-45 ◽  
Author(s):  
Greg Wetterau ◽  
Paul Liu ◽  
Bruce Chalmers ◽  
Tom Richardson ◽  
Heather Boyle VanMeter
Keyword(s):  
Design Criteria ◽  
Potable Reuse ◽  
Indirect Potable Reuse

scholarly journals Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice

2020 ◽  
Vol 11 ◽  
Author(s):  
Antonella Smeriglio ◽  
Marcella Denaro ◽  
Valeria D’Angelo ◽  
Maria Paola Germanò ◽  
Domenico Trombetta
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Molecular Mechanisms ◽  
Biological Effects ◽  
Ascorbic Acid Content ◽  
Zebrafish Embryos ◽  
Anti Inflammatory ◽  
Dose Dependent

Citrus juices are a rich source of bioactive compounds with various and well-known health benefits. The aim of this study was to investigate the polyphenols and ascorbic acid content as well as to investigate the antioxidant, anti-inflammatory and anti-angiogenic properties of the juice of an ancient Mediterranean species, Citrus lumia Risso (CLJ). The antioxidant and anti-inflammatory activities were evaluated by several in vitro cell-free and cell-based assays, whereas two different in vivo models, the chick chorioallantoic membrane (CAM) and the zebrafish embryos, were used to characterize the anti-angiogenic properties. Twenty-eight polyphenols were identified by RP-LC-DAD-ESI-MS analysis (flavonoids 68.82% and phenolic acids 31.18%) with 1-caffeoyl-5-feruloylquinic acid and kaempferol 3′-rhamnoside, which represent the most abundant compounds (25.70 and 23.12%, respectively). HPLC-DAD analysis showed a high ascorbic acid content (352 mg/kg of CLJ), which contributes with polyphenols to the marked and dose-dependent antioxidant and anti-inflammatory properties observed. CLJ showed strong and dose-dependent anti-angiogenic activity as highlighted by the inhibition of blood vessel formation on CAMs and the decrease of endogenous alkaline phosphatase on zebrafish embryos. Moreover, within the concentration range tested, no dead or malformed embryos were recorded. Certainly, further studies are needed to investigate the molecular mechanisms underlying these promising biological effects, but considering the evidence of the present study, the use of CLJ as a ready-to drink safe prevention strategy for inflammatory-based diseases correlated to angiogenesis could be justified.

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