Temperature influences the content and biosynthesis gene expression of saxitoxins (STXs) in the toxigenic dinoflagellate Alexandrium pacificum

2021 ◽  
pp. 149801
Author(s):  
Hui Wang ◽  
Hansol Kim ◽  
Hyunjun Park ◽  
Jang-Seu Ki
Keyword(s):  
Gene Expression ◽  
Biosynthesis Gene

scholarly journals Cell Wall Composition and Candidate Biosynthesis Gene Expression During Rice Development

2016 ◽  
Vol 57 (10) ◽  
pp. 2058-2075 ◽  
Author(s):  
Fan Lin ◽  
Chithra Manisseri ◽  
Alexandra Fagerström ◽  
Matthew L. Peck ◽  
Miguel E. Vega-Sánchez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Cell Wall Composition ◽  
Biosynthesis Gene

scholarly journals Light-Induced Carotenogenesis in Streptomyces coelicolor A3(2): Identification of an Extracytoplasmic Function Sigma Factor That Directs Photodependent Transcription of the Carotenoid Biosynthesis Gene Cluster

2005 ◽  
Vol 187 (5) ◽  
pp. 1825-1832 ◽  
Author(s):  
Hideaki Takano ◽  
Saemi Obitsu ◽  
Teruhiko Beppu ◽  
Kenji Ueda
Keyword(s):  
Gene Expression ◽  
Gene Cluster ◽  
Sigma Factor ◽  
Streptomyces Coelicolor ◽  
Regulatory Region ◽  
Carotenoid Biosynthesis ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene ◽  
Extracytoplasmic Function Sigma Factor

ABSTRACT Carotenoids are produced by a variety of organisms, but the mechanisms that regulate gene expression leading to carotenoid biosynthesis have been characterized for only a few organisms. In this study, we found that Streptomyces coelicolor A3(2), a gram-positive filamentous bacterium, produces carotenoids under blue light induction. The carotenoid fraction isolated from the cell extract contained multiple compounds, including isorenieratene and β-carotene. The carotenoid biosynthesis gene cluster of S. coelicolor consists of two convergent operons, crtEIBV and crtYTU, as previously shown for Streptomyces griseus. The crtEIBV null mutant completely lost its ability to produce carotenoids. The crt gene cluster is flanked by a regulatory region that consists of two divergent operons, litRQ and litSAB. The lit (light-induced transcription) genes encode a MerR-type transcriptional regulator (LitR), a possible oxidoreductase (LitQ), an extracytoplasmic function sigma factor (σLitS), a putative lipoprotein (LitA), and a putative anti-sigma factor (LitB). S1 protection assay revealed that the promoters preceding crtE (PcrtE), crtY (PcrtY), litR (PlitR), and litS (PlitS) are activated upon illumination. A litS mutant lost both the ability to produce carotenoids and the activities of PcrtE, PcrtY, and PlitS, which suggested that σLitS directs light-induced transcription from these promoters. An RNA polymerase holocomplex containing purified σLitS recombinant protein generated specific PcrtE and PcrtY transcripts in an in vitro runoff transcriptional assay. A litR mutant that had an insertion of the kanamycin resistance gene was defective both in the ability to produce carotenoids and in all of the light-dependent promoter activities. Overexpression of litS resulted in constitutive carotenoid production in both the wild type and the litR mutant. These results indicate that σLitS acts as a light-induced sigma factor that directs transcription of the crt biosynthesis gene cluster, whose activity is controlled by an unknown LitR function. This is the first report to describe light-inducible gene expression in Streptomyces.

scholarly journals Tight control of mycotoxin biosynthesis gene expression in Aspergillus flavus by temperature as revealed by RNA-Seq

2011 ◽  
Vol 322 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Jiujiang Yu ◽  
Natalie D. Fedorova ◽  
Beverly G. Montalbano ◽  
Deepak Bhatnagar ◽  
Thomas E. Cleveland ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aspergillus Flavus ◽  
Rna Seq ◽  
Tight Control ◽  
Biosynthesis Gene ◽  
Mycotoxin Biosynthesis

scholarly journals Juvenile Hormone Biosynthesis Gene Expression in the corpora allata of Honey Bee (Apis mellifera L.) Female Castes

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86923 ◽  
Author(s):  
Ana Durvalina Bomtorin ◽  
Aline Mackert ◽  
Gustavo Conrado Couto Rosa ◽  
Livia Maria Moda ◽  
Juliana Ramos Martins ◽  
...  
Keyword(s):  
Gene Expression ◽  
Apis Mellifera ◽  
Juvenile Hormone ◽  
Honey Bee ◽  
Corpora Allata ◽  
Juvenile Hormone Biosynthesis ◽  
Biosynthesis Gene ◽  
Hormone Biosynthesis
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