Mixotrophic denitrification for enhancing nitrogen removal of municipal tailwater: Contribution of heterotrophic/sulfur autotrophic denitrification and bacterial community

Author(s):  
Yingying Li ◽  
Ling Liu ◽  
Hongjie Wang
Chemosphere ◽  
2018 ◽  
Vol 193 ◽  
pp. 591-601 ◽  
Author(s):  
María J. García-Ruiz ◽  
Paula Maza-Márquez ◽  
Jesús González-López ◽  
Francisco Osorio

2019 ◽  
Vol 80 (2) ◽  
pp. 223-231
Author(s):  
Yixuan Xie ◽  
Ajun Wan ◽  
Xingmin Wang ◽  
Hengjie Dong ◽  
Yunpeng Wu

Abstract In this study, we use an anaerobic-aerobic integrated denitrification (Fe/C-ZACID) device with an iron-carbon-activated carbon and zeolite composite filter to remove nitrogen from simulated low carbon-nitrogen ratio (C/N) sewage. The impacts of dissolved oxygen (DO) level, hydraulic retention time (HRT), C/N and nitrate recirculation ratio on denitrification performance were studied. The results show that when HRT was 6 h, DO was 3 ± 0.1 mg/L, influent C/N was 3, and nitrate recirculation ratio was 100%, and removal rates of 95% for ammonia and 85% for total nitrogen (TN) were achieved. A beaker comparison test demonstrated that this synergistic denitrification system included heterotrophic denitrification, physicochemical denitrification, iron autotrophic denitrification and hydrogen autotrophic denitrification, etc. The Fe/C-ZACID device has a high-efficiency nitrogen removal effect for low C/N ratio sewage and strong shock resistance, which provides technical support and a theoretical basis for advanced denitrification of rural domestic sewage.


2012 ◽  
Vol 178-181 ◽  
pp. 549-552
Author(s):  
De Wei Mu

Bacterial community structure of advanced HA-A/A-MCO sludge reduction process is analyzed by DGGE fingerprinting technology. Research results indicates that microbial flora showed the distinction of highly diversity in anaerobic tank, anoxic tank and MCO tank of the HA-A/A-MCO process, and each tank has its own diversified and stable dominant microorganisms, as so the co-activation of such preponderant bacterial community promotes the system to exert favourable and consistent function of phosphorous and nitrogen removal and sludge reduction.


2006 ◽  
Vol 72 (2) ◽  
pp. 1364-1372 ◽  
Author(s):  
Ingrid Brettar ◽  
Matthias Labrenz ◽  
Sébastien Flavier ◽  
Julia Bötel ◽  
Harri Kuosa ◽  
...  

ABSTRACT Identification and functional analysis of key members of bacterial communities in marine and estuarine environments are major challenges for obtaining a mechanistic understanding of biogeochemical processes. In the Baltic Sea basins, as in many other marine environments with anoxic bodies of water, the oxic-anoxic interface is considered a layer of high bacterial turnover of sulfur, nitrogen, and carbon compounds that has a great impact on matter balances in the whole ecosystem. We focused on autotrophic denitrification by oxidation of reduced sulfur compounds as a biogeochemically important process mediating concomitant turnover of sulfur, nitrogen, and carbon. We used a newly developed approach consisting of molecular analyses in stimulation experiments and in situ abundance. The molecular approach was based on single-strand conformational polymorphism (SSCP) analysis of the bacterial community RNA, which allowed identification of potential denitrifiers based on the sequences of enhanced SSCP bands and monitoring of the overall bacterial community during the experiments. Sequences of the SSCP bands of interest were used to design highly specific primers that enabled (i) generation of almost complete 16S rRNA gene sequences using experimental and environmental DNA as templates and (ii) quantification of the bacteria of interest by real-time PCR. By using this approach we identified the bacteria responsible for autotrophic denitrification as a single taxon, an epsilonproteobacterium related to the autotrophic denitrifier Thiomicrospira denitrificans. This finding was confirmed by material balances in the experiments that were consistent with those obtained with continuous cultures of T. denitrificans. The presence and activity of a bacterium that is phylogenetically and physiologically closely related to T. denitrificans could be relevant for the carbon budget of the central Baltic Sea because T. denitrificans exhibits only one-half the efficiency for carbon dioxide fixation per mol of sulfide oxidized and mol of nitrate reduced of Thiobacillus denitrificans hypothesized previously for this function.


Sign in / Sign up

Export Citation Format

Share Document