scholarly journals Free radical scavenging activity, total phenolic content, total antioxidant status, and total oxidant status of endemic Thermopsis turcica

2013 ◽  
Vol 20 (3) ◽  
pp. 235-239 ◽  
Author(s):  
Laçine Aksoy ◽  
Erdi Kolay ◽  
Yasin Ağılönü ◽  
Zeyneb Aslan ◽  
Mustafa Kargıoğlu
Keyword(s):  
Antioxidant Status ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Antioxidant Status ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
Thermopsis Turcica ◽  
Oxidant Status

Total Phenolic Content and DPPH Free Radical Scavenging Activity of Young Turmeric Grown in Southern Thailand

2019 ◽  
Vol 886 ◽  
pp. 61-69
Author(s):  
Suriyan Sukati ◽  
Warachate Khobjai
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Methanolic Extracts ◽  
Southern Thailand

Turmeric (Curcuma longa L.), a member of Zingiberacaeae, is used for Thai traditional medicine, flavoring, preservative, and coloring agent. In Southern Thailand, turmeric is a famous ingredient for food recipes. Not only is the ripened rhizome used for cuisine, but also the young rhizomes. Previous studies have indicated that a variety of turmeric products from the ripened rhizome are valuable source of antioxidant compounds. However, there are ambiguous data regarding the level of antioxidant activity of the young rhizome. The aim of this study was to investigate the level of total phenolic content (TPC) and antioxidant activity of the young rhizomes by comparing to the ripened rhizome. TPC and antioxidant activity of aqueous and 80% methanolic extracts from the young turmeric grown in Southern Thailand were estimated using the Folin-Ciocalteu method and DPPH free radical scavenging activity (DPPH-RSA) assay, respectively. Results showed that the concentration of TPC determined in methanolic extract (19.80±0.25 mg GAE/ g extract) from the young rhizome was significantly higher than the corresponding aqueous extract (18.38±0.41 mg GAE/ g extract). The aqueous and methanolic extracts exhibited significant inhibition in DPPH-RSA with the half-maximal inhibitory concentration (IC50) value of 5.88±0.70 mg/mL and 3.00±0.31 mg/mL, respectively. However, in comparison with the ripened rhizome, TPC and DPPH-RSA of the young rhizome were lower. These results indicate that the young rhizome grown in Southern Thailand could be a source of antioxidant compounds, but the ripened rhizome provides better antioxidant properties than the young rhizome.

scholarly journals Artemisia dracunculus Essential Oil Chemical composition and antioxidant properties

2019 ◽  
Vol 70 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Ariadna Petronela Fildan ◽  
Ioan Pet ◽  
Daniela Stoin ◽  
Gabriel Bujanca ◽  
Alexandra Teodora Lukinich-Gruia ◽  
...  
Keyword(s):  
Free Radical ◽  
Chemical Composition ◽  
Essential Oil ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Artemisia Dracunculus

Free radical scavenging activity, total phenolic content and the chemical composition of the essential oil isolated by steam distillation from Artemisia dracunculus L. was investigated. The isolation yield was 0.24% (v/w) based on the fresh plant material (leaves). GC-MS investigation identified 21 components, accounting 99.93% of the total amount. The major components were sabinene (42.38%), isoelemicin (12.91%), methyl eugenol (9.09%), elemicin (7.95%) and beta-ocimene (6.46%). The free radical scavenging activity of the essential oil of Artemisia dracunculus L. was evaluated in vitro by the DPPH assay (IC50 = 0.730 � 0.213 mg/mL), BHA and alpha-tocopherol were used as a positive control. The total phenolic content of the tarragon essential oil was evaluated by the Folin-Ciocalteu method (GAE = 0.451 � 0.001 mg/g sample). In view of these data, we consider that tarragon essential oil could represent a new antioxidants source as a reliable option to reduce the usage of synthetic additives.

scholarly journals Isolasi dan Aktivitas Antioksidan Fraksi dari Ekstrak Tongkol Jagung

2017 ◽  
Vol 37 (2) ◽  
pp. 139
Author(s):  
Edi Suryanto ◽  
Lidya Irma Momuat
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Corn Cob ◽  
Total Antioxidant

The corn cob is one of the food waste-material having phytochemical component that of health benefit. The objectives of the research were to isolate phenolic fractions of the corn cob extract and to determine antioxidant and antiphotooxidant activities. The corn cob was extracted with reflux method using ethanol 80% for 2 hours. After that the extracts were filtered and the filtrates were combined and concentrated on rotary evaporator. This crude ethanolic extract was suspended in water and extracted with petroleum ether, ethyl acetate, n-butanol and water, respectively. The best fractions of partition fractionated with colomn chromatography using silica gel 60 and n-hexane: ethyl acetate (4:6). Corncob extract and fraction were evaluated for total phenolic content free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl radical assay) and total antioxidant capacity (ferric reducing ability of plasma assay). The results of total phenolic content showed that ethyl acetate fraction (163.57 μg/mL) has higher phenolic content than butanol fraction (83.30 μg/mL), ethanol extract (81.53 μg/mL), water fraction (23.71 μg/mL) and petroleum ether fraction (23.57 μg/mL). The results also showed that ethyl acetate fraction has highest free radical scavenging activity and total antioxidant capacity than butanol fraction, ethanol extract, water fraction and petroleum ether fraction. The free radical scavenging activity of fractions II and III exhibited strongest activity than fraction V, I, IV and VI. The results also showed that fraction II has highest total antioxidant capacity than I, III, IV, VI, and V. The results suggest that the ethyl acetate fraction and fraction II contains phenolic compounds which possess antioxidant activities. ABSTRAKTongkol jagung merupakan salah satu limbah tanaman pangan yang mempunyai kandungan fitokimia yang bermanfaat untuk kesehatan. Penelitian ini bertujuan untuk mengisolasi fraksi fenolik dari ekstrak tongkol jagung dan menentukan aktivitas antioksidan. Tongkol jagung diekstraksi dengan cara refluk menggunakan etanol 80% selama 2 jam. Setelah itu disaring dan filtratnya diuapkan dengan rotary evaporator. Ekstrak etanol disuspensikan dalam air dan diekstraksi berturut turut dengan petroleum eter, etil asetat, butanol, dan air. Fraksi pelarut terbaik difraksinasi dengan kromatografi kolom menggunakan silika gel 60 dan eluen n-heksana : etil asetat (4:6). Ekstrak tongkol jagung dan fraksi dievaluasi kandungan total fenolik, aktivitas penangkal radikal bebas (uji 1,1-difenil-2-pikrilhidrazil) dan kapasitas total antioksidan (uji ferric reducing ability of plasma). Hasil uji kandungan total fenolik menunjukkan bahwa fraksi etil asetat (163,57 μg/mL) memiliki kandungan total fenolik yang paling tinggi daripada fraksi butanol (83,30 μg/mL), ekstrak etanol (81,53 μg/mL), fraksi air (23,71 μg/mL) dan fraksi petroleum eter (23,57 μg/mL). Hasil ini juga menunjukkan bahwa fraksi etil asetat mempunyai aktivitas penangkal radikal dan kapasitas total antioksidan paling tinggi daripada fraksi butanol, ekstrak etanol, fraksi air, dan fraksi petroleum eter. Aktivitas penangkal radikal bebas fraksi II dan III memperlihatkan paling kuat daripada fraksi V, I, IV dan VI. Hasil penelitian ini menyarankan bahwa fraksi etil asetat dan fraksi II mengandung senyawa fenolik yang mempunyai aktivitas antioksidan kuat.

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