Molecular Docking Senyawa Ekstrak Etanol Daun Kenikir (Cosmos caudatus) Sebagai Inhibitor Il-6 Dalam Respon Inflamasi

Inflamasi merupakan reaksi alami tubuh untuk melindungi diri dari patogen menyebab penyakit. Interleukin-6 (IL-6) berperan sebagai sitokin pro-inflamasi dan antiinflamasi yang dikeluarkan oleh sel T dan makrofag untuk merangsang respon imun saat terjadi infeksi. Pada penelitian ini, dilakukan studi in silico ekstrak etanol daun kenikir (Cosmos caudatus) sebagai anti-inflamasi yang didasarkan pada afinitas terhadap Interleukin-6 (IL-6) dengan menggunakan metode molecular docking secara in silico. Penelitian ini meliputi simulasi penambatan molekuler kandungan senyawa ekstrak etanol daun kenikir terhadap reseptor IL-6 (PDB ID : 1ALU) dan prediksi profil farmakokinetik dan toksisitas menggunakan server Pre-ADMET. Hasil penelitian menunjukkan bahwa diperoleh empat senyawa potensial, yaitu C1 (Kaempferol), C2 (1-Caffeoylquinic acid, C3 (Procyanidin B1), dan C5 (Quercetin-3-O-pentoside).Keempat senyawa tersebut memiliki nilai ?G dan Ki yang lebih baik dibandingkan dengan ligan alami. Nilai ?G; Ki senyawa uji tersebut, yaitu berturut-turut (-5,29 Kkal/mol; 133,31 uM), (-6,02 Kkal/mol; 38,44 uM), (-5,38 Kkal/mol; 114,24 uM), dan (-5,46 Kkal/mol; 99,50 uM). Hasil PreADMET keempat senyawa tersebut memiliki daya adsorpsi yang baik sehingga dapat dipertimbangkan dalam pembuatan sediaan rute oral. Hasil ames test menunjukkan bahwa C1 dan C2 bersifat mutagenik, sedangkan C3 dan C4 non mutagenik.

Pertumbuhan dan Produksi Pucuk Kenikir pada Beberapa Komposisi Media Tanam dan Interval Pemanenan (The Growth and Shoot Production of Cosmos caudatus with Various Plant Media Composition and Harvesting Interval)

<p>Kenikir dapat dimanfaatkan sebagai sayuran dengan cara mengonsumsi pucuknya. Kenikir banyak dijumpai sebagai tumbuhan liar sehingga diperlukan upaya untuk meningkatkan produksi pucuk kenikir. Produksi pucuk kenikir dapat dioptimalkan dengan melakukan perbaikan teknik budidaya, salah satunya pengaturan komposisi media tanam dan interval panen pucuk. Tujuan dari penelitian ini adalah untuk menganalisis pengaruh komposisi media tanam dan interval pemanenan terhadap pertumbuhan dan produksi pucuk kenikir. Penelitian dilaksanakan pada bulan Februari sampai dengan Mei 2019 di Kebun Percobaan Fakultas Pertanian UGJ, Cirebon. Percobaan dilakukan dalam dua tahap, yaitu pengaruh komposisi media tanam terhadap pertumbuhan dan produksi pucuk kenikir pada panen pertama serta pengaruh interaksi antara komposisi media tanam dan interval pemanenan terhadap produksi pucuk pada panen kedua. Rancangan percobaan pada percobaan pertama menggunakan Rancangan Acak Kelompok (RAK) satu faktor, yaitu komposisi media tanam dengan sembilan ulangan dan rancangan percobaan pada percobaan kedua menggunakan RAK faktorial dua faktor. Faktor pertama adalah komposisi media tanam dan faktor kedua adalah interval pemanenan dan diulang sebanyak tiga kali. Komposisi media tanam terdiri atas tanah, tanah : pukan (2:1), dan tanah : pukan (1:1). Interval pemanenan yang dicobakan terdiri atas tiga taraf, yaitu 10, 15, dan 20 hari. Hasil penelitian menunjukkan bahwa media tanam tanah : pukan (1:1) meningkatkan pertumbuhan dan produksi pucuk kenikir baik pada saat panen pertama maupun panen kedua. Interval pemanenan 20 hari menurunkan produksi pucuk total disebabkan interval pemanenan yang terlalu lama menyebabkan banyaknya pucuk kenikir yang berbunga sehingga menjadi tidak layak untuk dipanen.</p><p><strong>Keywords</strong></p><p>Interval pemanenan; Kenikir (Cosmos caudatus); Media tanam; Pertumbuhan; Produksi pucuk</p><p><strong>Abstract</strong></p><p>Cosmos caudatus can be used as vegetable by its shoots. Cosmos are found as wild plant so that efforts are needed to increase shoot production. Shoot production can be optimized by media composition and harvesting interval. This research objective was to investigate planting media composition and harvesting interval effect on growth and shoot production of cosmos. The research was carried out for three months at Cirebon. The experiment was carried out in two stages: (1) the effect of media composition on growth and shoot at the first harvest and (2) the effect of interaction between media composition and shoot harvesting interval at the second harvest. The experimental design for first experiment used Randomized Block Design (RBD), namely the composition of media of soil, 2:1 soil-manure and 1:1 soil-manure with nine replications. Meanwhile, we used two factor factorial RBD for second experiment. The first factor was the media composition and the second factor was three harvest interval with three replications. The result showed that planting media of soil and manure (1:1) increased the growth and shoot both in the first and second harvesting. The harvesting interval of 20 days decrease the total shoot because many branches to flower so they cannot be harvested.</p>

Agrobiodiversity of using refugia plants towards several plant gardens at Tulung Rejo, East Java

A study and identificiation on agrobiodiversity of refugia plant as alternative solution by farmers towards their several plant gardens to repel pest was conducted at Tulung Rejo, East Java, Indonesia in March 2021. Refugia plants are biodiversity plants that grow around cultivated plants, which have the potential as a place of protection and a source of food for natural enemy insects (both predators and parasitoids). The principle of refugia pants is that these plants can attract and become a place of life and a source of life for insects, natural enemies of pests. the reseach was using a survey method with interview with farmers who have a land and using refugia plant as a natural pesticides to repel pests of their plants. besides, this research was also using a physical indentification of several refugia plants. Among several locations in Tulung Rejo, the results show that on average farmers use refugia plants of the type of kenikir flower Cosmos caudatus. This has evident because from 20 garden locations, 18 of them use Cosmos caudatus. The needs of farmers in Tulung Rejo, East Java to repel pests naturally.

PENGARUH UMUR PANEN PUCUK DAN KONSENTRASI POC URINE KELINCI TERHADAP PERTUMBUHAN DAN PRODUKSI PUCUK KENIKIR (Cosmos caudatus)

Kenikir is an indigenous plant that has the potential to be cultivated, but there are still many people who do not know the potential of kenikir. This research aims to study the effect of shoots harvest on the results of kenikir and to find the best concentration of liquid organic fertilizer rabbit urine for the growth of kenikir. This research was conducted in January - April 2020 in the experimentation field of Perjuangan University of Tasikmalaya at an altitude of 359 m asl. The design used was a two factor Randomized Complete Block Design (RCBD). The first factor is the age of harvest, consisting of 3 levels ie 7 WAP (Weeks after planting), 8 WAP and 9 WAP. The second factor is urine concentration consisting of 4 levels ie 0 ml.L-1, 10 ml.L-1, 20 ml.L-1 and 30 ml.L-1. The results showed that 10 ml of L-1 U2 treatment produced higher kenikir plants and thicker diameters. U2P3 treatment gave the best response to plant height variables. All treatment combinations gave the same response to the number of leaves harvested and the weight of fresh shoots

Enhancing recovery of bioactive compounds from Cosmos caudatus leaves via ultrasonic extraction

Cosmos caudatus (C. caudatus) is a medicinal plant that is high in bioactive compounds such as phenolics. In this study, an ultrasound extraction method was used to optimise the extraction of bioactive compounds from C. caudatus leaves. Response surface methodology (RSM) based on a Box-Behnken design (BBD) was applied to obtain the optimum extraction parameters which is solid–liquid ratio (10–30 g/mL), particle size (180–850 µm) and extraction time (20–30 min) for maximal quercitrin and total phenolic content (TPC) yields. Analysis of antimicrobial activity was performed against two human pathogenic microbes: Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) by the agar well diffusion method. The optimal ultrasonic extraction condition was as follow: solvent-liquid ratio of 1:28 (g/mL), particle size of 485 µm, and duration of 30 min, respectively. Remarkably, extraction using ultrasonic method had recovered more bioactive content and antioxidant activity than the Soxhlet method. The extract also exhibited good antimicrobial activities. Due to the above findings, the ultrasonic extraction was found to be suitable to improve recovery extraction of quercitrin and TPC from C. caudatus leaves. It also opens the possibility that the plant extract can be used for functional food and antimicrobial agents in various applications.

An Insight of Co-Encapsulation Nigella sativa and Cosmos caudatus Kunth Extracts as Anti-Inflammatory Agent Through In Silico Study

This study analyzes anti-inflammatory activity from extracts of Nigella sativa and Cosmos caudatus Kunth co-encapsulated through in silico molecular docking. The LC-MS results revealed that extracts of N. sativa mostly contained thymoquinone and alpha-hederin, whereas quercetin and kaempferol were the major compounds in C. caudatus K. Nevertheless, the bioactive compounds are usually susceptible to degradation by exposure to light, heat, oxygen, which may limit its biological activity. Therefore, encapsulation is one of the promising techniques to protect bioactive compounds. Ligands were encapsulated with chitosan and sodium tripolyphosphate as wall materials. Cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) as the target enzymes were docked with a combination of these active compounds (non-encapsulated and encapsulated), using the HEX 8.0 program, and visualized using the Discovery studio visualizer software v16.1.0.15350. Interestingly, docking results of the combination of encapsulated ligands showed no interactions to COX-1 but interacted with COX-2. Therefore, co-encapsulation of extracts combinations has been suggested to act as anti-inflammatory agents targeted specifically to the COX-2 enzyme. The total energy of the encapsulated of combination of extract compounds to COX-2 were -1425.88 (mol/cal) for thymoquinone + quercetin; -1435.87 (mol/cal) for thymoquinone + kaempferol; 1175.97 (mol/cal) for quercetin + alpha hederin; -957.74 (mol/cal) for kaempferol + alpha hederin; and -283.3 (mol/cal) for diclofenac sodium, as a control NSAID drug. These suggest that encapsulated active compounds in N. sativa and C. caudatus K. have potency as a drug candidate for the selective NSAIDs category, which can be subjected to further in vitro and in vivo studies.

Effect of encapsulated Cosmos caudatus leaf extract on the physiological conditions, immune competency, and antioxidative status of broilers at high stocking density

The present study aimed to investigate the effect of encapsulated Cosmos caudatus leaf extract on the physiological conditions, immune competency, and antioxidative status of broiler chickens raised at a high stocking density. After 15 days of rearing, 370 Lohmann broiler chicks were assigned to five treatment groups, including T0 (chicks were raised at a density of 10 birds/m2 and received no additive), T1 (chicks were raised at a density of 16 birds/m2 and received no additive), T2 (chicks were raised at a density of 16 birds/m2 and received 0.5 g/kg encapsulated C. caudatus leaf extract), T3 (chicks were raised at a density of 16 birds/m2 and received 1.0 g/kg additive), and T4 (chicks were raised at a density of 16 birds/m2 and received 1.5 g/kg additive). On days 28 and 42, blood samples from two chicks per pen were collected. On day 42, the chicks that had been blood-sampled were sacrificed, and blood samples and lymphoid organs (i.e., bursa of Fabricius, spleen, and thymus) were collected. The daily weight gain and feed efficiency of broilers (P < 0.01) in groups T2 and T3 were higher than those of broilers in groups T0, T1, and T4. Daily feed intake was greater (P < 0.01) in groups T0 and T1 than in groups T2 and T3. The erythrocyte content and hematocrit value of groups T1, T2, T3, and T4 were greater (P < 0.05) than those of group T0. The mean corpuscular hemoglobin concentration in group T4 was lower (P < 0.05) than that in groups T0, T1, and T2. Leukocyte and lymphocyte levels were higher in group T1 (P < 0.05) than in other groups. Serum albumin was higher in chicks reared at a high density (P < 0.05) than in chicks reared at a normal density. Lesion scores were higher in group T1 (P < 0.05) than in other groups. Chicks in groups T1 and T2 showed more severe pathological changes in their bursa of Fabricius compared with those in groups T0, T3, and T4. Serum superoxide dismutase was higher in groups T2, T3, and T4 (P < 0.05) than in groups T0 and T1. Chicks in group T4 had higher (P < 0.05) malondialdehyde levels than chicks in other groups. In conclusion, a high stocking density influences the metabolic rate and physiological conditions of broiler chicks, as reflected by alterations in the blood profiles of the animals. Stress due to a high stocking density could damage the bursa of Fabricius, but feeding with encapsulated C. caudatus leaf extract, especially at a rate of 1.5 g/kg, could alleviate the cortical and lymphocyte cell depletion of broilers. Regardless of the stocking density effect, dietary supplementation with encapsulated C. caudatus leaf extract at doses of 0.5 and 1.0 g/kg could improve the daily weight gain of broilers.