Effects of laser needle-knife therapy on vertebroarterial morphology and protein expression of PI-3K, AKT and VEGF in the carotid artery in a rabbit model of cervical spondylotic arteriopathy

AbstractOne of the main challenges of tissue-engineered vascular prostheses is restenosis due to intimal hyperplasia. The aim of this study is to develop a material for scaffolds able to support cell growth while tolerating physiological conditions and maintaining the patency of carotid artery model. Tubular hyaluronic acid (HA)-functionalized collagen nanofibrous composite scaffolds were prepared by sequential electrospinning method. The tubular composite scaffold has well-controlled biophysical and biochemical signals, providing a good matrix for the adhesion and proliferation of vascular endothelial cells (ECs), but resisting to platelets adhesion when exposed to blood. Carotid artery replacement experiment from 6-week rabbits showed that the HA/collagen nanofibrous composite scaffold grafts with endothelialization on the luminal surface could maintain vascular patency. At retrieval, the composite scaffold maintained good structural integrity and had comparable mechanical strength as the native artery. This study indicating that electrospun scaffolds combined with cells may become an alternative to prosthetic grafts for vascular reconstruction. Graphical Abstract

Download Full-text

Simulated microgravity effects on the rat carotid and femoral arteries: role of contractile protein expression and mechanical properties of the vessel wall

Journal of Applied Physiology ◽

10.1152/japplphysiol.01020.2006 ◽

2007 ◽

Vol 102 (4) ◽

pp. 1595-1603 ◽

Cited By ~ 13

Author(s):

Sunup Hwang ◽

Stanislav A. Shelkovnikov ◽

Ralph E. Purdy

Keyword(s):

Carotid Artery ◽

Protein Expression ◽

Femoral Artery ◽

Vessel Wall ◽

Male Rats ◽

Force Analysis ◽

Western Blots ◽

Active Length ◽

Femoral Arteries ◽

Protein Levels

The goal of this study was to determine the effects of microgravity on myofilament protein expression and both passive and active length-force relationships in carotid and femoral arteries. Microgravity was simulated by 20-day hindlimb unweighting (HU) in Wistar male rats, and carotid and femoral artery segments were isolated from both HU and control (CTL) rats for Western blot and length-force analysis. Western blots revealed that HU significantly decreased myosin light chain-20 (MLC-20) protein levels in both carotid and femoral arteries and decreased myosin heavy chain (MHC) in femoral artery. α-Actin levels were not altered by HU treatment in either artery. Length-force analysis demonstrated that HU did not change either passive or active length-force relationships in the femoral artery. HU-treated arterial rings developed significantly less force to 100 mM K+ than CTL, but optimal lengths were identical. In the carotid artery, length-active force curves were identical for both CTL and HU; however the length-passive force curve for HU-treated rings exhibited a steeper slope than CTL, suggesting decreased compliance of the artery wall. In conclusion, our data suggest that the HU-induced decreases in both MLC-20 and MHC in femoral artery are responsible for the decreased contraction to 100 mM K+ in HU-treated femoral artery rings. In the carotid artery, the HU-induced decrease in vessel wall compliance may counter any decrease in contractility caused by the decreased MLC-20 levels.

Download Full-text

Abstract 117: Heparin Derived Oligosaccharide Inhibits Vascular Intimal Hyperplasia in Balloon Injured Carotid Artery and the Mechanism Involved

Circulation Research ◽

10.1161/res.117.suppl_1.117 ◽

2015 ◽

Vol 117 (suppl_1) ◽

Author(s):

He Shuying ◽

Wu Jie

Keyword(s):

Growth Factor ◽

Carotid Artery ◽

Common Carotid Artery ◽

Intimal Hyperplasia ◽

Serum Lipids ◽

Rabbit Model ◽

High Cholesterol Diet ◽

Type I ◽

Monocyte Chemoattractant Protein 1 ◽

Expression Levels

To determine the effects of heparin-derived oligosaccharides (HDOs) on vascular intimal hyperplasia (IH) in balloon-injured carotid artery and the mechanism involved. The animal model was established by rubbing the endothelia within the common carotid artery (CCA) of male rabbits along with a high-cholesterol diet. The arterial IH was testified by histopathological changes of the CCA. Serum lipids were detected using automated biochemical analysis; Expression of mRNAs corresponding to vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), vascular cell adhesion molecule-1 (VCAM-1), monocyte chemoattractant protein-1 (MCP-1), scavenger receptor class B type I (SR-BI) and ATP-binding cassette transporter A1 (ABCA-1) were analyzed using reverse transcription polymerase chain reaction assays. Expressions of VEGF, VCAM-1, MCP-1, SR-BI and ABCA-1 proteins were detected by western blotting. Enzyme-linked immunosorbent assays were used to quantify expression levels of VEGF and bFGF. The results implied that administration of HDO significantly inhibited common carotid artery histopathology and restenosis that was induced by balloon injury. Treatment with HDO also significantly decreased mRNA and protein expression levels of VEGF, bFGF, VCAM-1, MCP-1, and SR-BI in the arterial wall, however ABCA-1 expression levels were elevated. HDO treatment led to a reduction in various serum lipids (total cholesterol, triglycerides, high-density and low-density lipoproteins). We concluded that, in a rabbit model, HDO can ameliorate IH and the mechanisms might involved VEGF, bFGF, VCAM-1, MCP-1, SR-BI and ABCA-1.

Download Full-text

Longitudinal effects of common carotid artery stenosis on ocular hemodynamics assessed using laser speckle flowgraphy in a rabbit model

Scientific Reports ◽

10.1038/s41598-020-72556-9 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Aishah Ismail ◽

Hui Cheng Chen ◽

Ibrahima Faye ◽

Tong Boon Tang

Keyword(s):

Carotid Artery ◽

Common Carotid Artery ◽

Carotid Artery Stenosis ◽

Rabbit Model ◽

Laser Speckle ◽

Artery Stenosis ◽

Laser Speckle Flowgraphy ◽

Stroke Severity ◽

Post Surgery ◽

The Impact

Abstract Real-time impairment of ocular blood flow (OBF) under common carotid artery stenosis (CCAS) has not been ascertained. We aimed to longitudinally assess the impact of CCAS on OBF using a rabbit model. About 75% stenosis was created by tying the common carotid artery with a plastic mandrel using a nylon suture. The plastic mandrel was gently removed, leaving a ligature. Neurological and behavioral assessments were recorded as the clinical indicator of stroke severity. With laser speckle flowgraphy, the pulse waveform parameters namely mean blur rate (MBR), blowout score (BOS), blowout time (BOT), rising rate, S1-area, falling rate (FR), S2-area, flow acceleration index (FAI), acceleration time index, resistive index (RI) and the difference between the maximum and minimum values of MBR (AC) were assessed in overall, vessel, and tissue regions of the optic nerve head (ONH). Longitudinally, BOS significantly increased until day 19 post-surgery, whereas FAI, RI, and AC significantly decreased. Beyond day 19, BOS, BOT, FR, FAI, RI, and AC significantly decreased. We defined two stages representing impaired vessel conditions, namely the vessel resistance phase, where BOS increases and FAI, RI, and AC decrease, and the vessel elasticity phase where BOS, BOT, FR, FAI, RI and AC decrease. These stages provide information about atherosclerosis, assessable non-invasively through the eye.

Download Full-text

Therapeutic application of repeated extracorporeal shock wave accompanied by microbubbles and thrombolytic agent administration in the rabbit model of embolic common carotid artery occlusion

Atherosclerosis ◽

10.1016/j.atherosclerosis.2015.04.347 ◽

2015 ◽

Vol 241 (1) ◽

pp. e99

Author(s):

H. Mehrad ◽

L. Kazemi

Keyword(s):

Shock Wave ◽

Carotid Artery ◽

Common Carotid Artery ◽

Thrombolytic Agent ◽

Rabbit Model ◽

Artery Occlusion ◽

Carotid Artery Occlusion ◽

Therapeutic Application ◽

Extracorporeal Shock Wave ◽

Common Carotid Artery Occlusion

Download Full-text

Therapeutic Effects and Finite Element Analysis of a Combined Treatment Using Laser Needle-Knife with Supine Repositioning Massage on Patients with Cervical Spondylotic Vertebral Arteriopathy

International Journal of Pattern Recognition and Artificial Intelligence ◽

10.1142/s0218001417570087 ◽

2017 ◽

Vol 31 (11) ◽

pp. 1757008 ◽

Cited By ~ 6

Author(s):

Fang Liu ◽

Wei Wei ◽

Gaoyi Yang ◽

Cunxin Wang ◽

Xin Yang ◽

...

Keyword(s):

Finite Element Analysis ◽

Finite Element ◽

Combined Treatment ◽

Maximum Flow ◽

Left Vertebral Artery ◽

Therapeutic Effects ◽

Element Analysis ◽

Laser Needle ◽

Needle Knife ◽

The Right

Objective: To investigate the effectiveness of combined laser needle-knife and massage on cervical spondylotic vertebral arteriopathy patients. Summary of background data: With the recent rise of electronic businesses, the incidence of cervical spondylosis has also risen rapidly. Methods: Cervical spondylotic vertebral arteriopathy patients were treated using laser needle-knife with massage, and compared to patients who only received the massage. A 3D anatomical and hemodynamic model was developed. Results: The symptomatic and functional overall scores were reduced by 71.43% after the combined treatment. Results from the finite element analysis indicated that the maximum flow rate of the left vertebral artery was improved by 47.52% and the right was improved by 38.67%. Conclusion: A combined treatment of cervical spondylotic vertebral arteriopathy using laser needle-knife and massage is an effective approach with a therapeutic mechanism closely related to hemodynamics.

Download Full-text